Cell co-culture method for improving proliferation activity of somatic cells

A technology of proliferative activity and somatic cells, applied in the field of cell co-culture to improve the proliferative activity of somatic cells, can solve the problems of tumor formation and inability to remove, and achieve the effects of simple steps, stable results and reliable principles

Inactive Publication Date: 2012-07-11
ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has the following defects: 1. The undifferentiated embryonic stem cells present in the transplanted cells will form tumors in the transplanted body
2. Stem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1:

[0029] The purpose of this example is to obtain a sufficient amount of highly active corneal epithelial cells in a short time. [Note: The suicide genes used in this example include Thymidine kinase (TK) gene, Cytosine deaminase (CD) ) Gene, E.coli-gpt, cytochrome P450 (Cytochrome P450 / cytochrome P450reductase) gene, nitroreductase (Nitroreductase) gene, carboxypeptidase (Carboxypeptidase G2) gene, e.coli-DeoD gene (expressing purine nucleoside) Phosphorylase), diphtheria toxin gene (DTA gene) and FAS gene and other suicide genes. Stem cells are one or more of embryonic stem cells or adult stem cells. Transfection methods include viruses and One or more non-viral transfection methods, the time to activate the suicide gene is one or more times, and the drugs that activate the suicide gene are acyclovir (ACV) and ganciclovir (ganciclovir, GCv) , 5-Fluorocytosine, 6-mercaptoxanthine, cyclophosphamide, 6-methylpurine-2-deoxyribonucleoside, ara-M, BVDU, CPA, IFO, C...

Example Embodiment

[0035] Example 2:

[0036] The purpose of this example is to obtain a sufficient amount of highly active human skin epidermal cells in a short time. [Note: The suicide genes used in this example include Thymidine kinase (TK) gene and Cytosine deaminase (Cytosine deaminase, CD) gene, E.coli-gpt, Cytochrome P450 (Cytochrome P450 / cytochrome P450reductase) gene, Nitroreductase gene, Carboxypeptidase G2 gene, e.coli-DeoD gene (expressing purine nucleus) Glycoside phosphorylase), diphtheria toxin gene (DTA gene) and FAS gene and other suicide genes. Stem cells are one or more of embryonic stem cells or adult stem cells. Transfection methods include viruses And one or more of non-viral transfection methods, the time to activate the suicide gene is one or more times, and the drugs that activate the suicide gene are acyclovir (ACV), ganciclovir (ganciclovir, GCv) ), 5-fluorocytosine, 6-mercaptoxanthine, cyclophosphamide, 6-methylpurine-2-deoxyribonucleoside, ara-M, BVDU, CPA, IFO, CB1954...

Example Embodiment

[0044] Example 3:

[0045] The purpose of this example is to obtain a sufficient amount of highly active cat corneal endothelial cells in a short time. [Note: The suicide genes used in this example include Thymidine kinase (TK) gene, Cytosine deaminase (CD) gene, E. coli-gpt, Cytochrome P450 (Cytochrome P450 / cytochrome P450 reductase) ) Gene, Nitroreductase gene, Carboxypeptidase G2 gene, e.coli-DeoD gene (express purine nucleoside phosphorylase), diphtheria toxin gene (DTA gene), FAS gene, etc. One or more kinds of suicide genes, the stem cells are one or more of embryonic stem cells or adult stem cells, the transfection method includes one or more of viral and non-viral transfection methods, and the suicide gene is activated The time is one or more times. The drugs that initiate the suicide gene are acyclovir (ACV), ganciclovir (GCv), 5-fluorocytosine, 6-mercaptoxanthine, cyclophosphamide, 6 -Methylpurine-2-deoxyribonucleoside, ara-M, BVDU, CPA, IFO, CB1954, MeP-dR, F-araA, C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a cell co-culture method for improving the proliferation activity of somatic cells. The cell co-culture method comprises the following steps of: transfecting suicide genes into stem cells; co-culturing the somatic cells and the stem cells transfected with the suicide gene; and after the somatic cells are proliferated, starting the suicide genes in the stem cells to remove the stem cells. The stem cells carrying the suicide genes and the somatic cells are co-cultured, the proliferation activity of the somatic cells is improved at the presence of the stem cells, a small number of somatic cells can quickly grow in short time, and sufficient somatic cells are obtained; and when the number of the somatic cells is large enough, the suicide genes carried in the stem cells are started to induce the apoptosis of the stem cells, and the pure somatic cells with high proliferation activity are obtained, and do not have tumorigenicity after transplantation, so that the method is great breakthrough for cell engineering and tissue engineering, and is a new way for next-generation clinical application. The method has the advantages of reliable principle, simple steps and stable results, and is easily applied to experimental study and clinical treatment in the field of bioengineering.

Description

Technical field: [0001] The invention belongs to the field of cell engineering and tissue engineering, and in particular relates to a cell co-cultivation method for improving the proliferative activity of somatic cells. Background technique: [0002] In the field of cell engineering and tissue engineering, a certain number of seed cells-somatic cells are required, and somatic cells are required to have certain proliferative activity, both of which are crucial to the restoration and reconstruction of the structure and function of tissues and organs in the field of bioengineering. important influence. However, due to the limited ability of somatic cells to proliferate in vitro, a small amount of somatic cells cannot obtain a sufficient amount of somatic cells through proliferation, and after somatic cells are cultured in vitro for a long time, with the increase in the number of divisions, the proliferation ability gradually decreases, and the proliferation activity of the cell...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/07C12N5/10
Inventor 王智崇周强武征
Owner ZHONGSHAN OPHTHALMIC CENT SUN YAT SEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products