Cell co-culture method for improving proliferation activity of somatic cells
A technology of proliferative activity and somatic cells, applied in the field of cell co-culture to improve the proliferative activity of somatic cells, can solve the problems of tumor formation and inability to remove, and achieve the effects of simple steps, stable results and reliable principles
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[0028] Example 1:
[0029] The purpose of this example is to obtain a sufficient amount of highly active corneal epithelial cells in a short time. [Note: The suicide genes used in this example include Thymidine kinase (TK) gene, Cytosine deaminase (CD) ) Gene, E.coli-gpt, cytochrome P450 (Cytochrome P450 / cytochrome P450reductase) gene, nitroreductase (Nitroreductase) gene, carboxypeptidase (Carboxypeptidase G2) gene, e.coli-DeoD gene (expressing purine nucleoside) Phosphorylase), diphtheria toxin gene (DTA gene) and FAS gene and other suicide genes. Stem cells are one or more of embryonic stem cells or adult stem cells. Transfection methods include viruses and One or more non-viral transfection methods, the time to activate the suicide gene is one or more times, and the drugs that activate the suicide gene are acyclovir (ACV) and ganciclovir (ganciclovir, GCv) , 5-Fluorocytosine, 6-mercaptoxanthine, cyclophosphamide, 6-methylpurine-2-deoxyribonucleoside, ara-M, BVDU, CPA, IFO, C...
Example Embodiment
[0035] Example 2:
[0036] The purpose of this example is to obtain a sufficient amount of highly active human skin epidermal cells in a short time. [Note: The suicide genes used in this example include Thymidine kinase (TK) gene and Cytosine deaminase (Cytosine deaminase, CD) gene, E.coli-gpt, Cytochrome P450 (Cytochrome P450 / cytochrome P450reductase) gene, Nitroreductase gene, Carboxypeptidase G2 gene, e.coli-DeoD gene (expressing purine nucleus) Glycoside phosphorylase), diphtheria toxin gene (DTA gene) and FAS gene and other suicide genes. Stem cells are one or more of embryonic stem cells or adult stem cells. Transfection methods include viruses And one or more of non-viral transfection methods, the time to activate the suicide gene is one or more times, and the drugs that activate the suicide gene are acyclovir (ACV), ganciclovir (ganciclovir, GCv) ), 5-fluorocytosine, 6-mercaptoxanthine, cyclophosphamide, 6-methylpurine-2-deoxyribonucleoside, ara-M, BVDU, CPA, IFO, CB1954...
Example Embodiment
[0044] Example 3:
[0045] The purpose of this example is to obtain a sufficient amount of highly active cat corneal endothelial cells in a short time. [Note: The suicide genes used in this example include Thymidine kinase (TK) gene, Cytosine deaminase (CD) gene, E. coli-gpt, Cytochrome P450 (Cytochrome P450 / cytochrome P450 reductase) ) Gene, Nitroreductase gene, Carboxypeptidase G2 gene, e.coli-DeoD gene (express purine nucleoside phosphorylase), diphtheria toxin gene (DTA gene), FAS gene, etc. One or more kinds of suicide genes, the stem cells are one or more of embryonic stem cells or adult stem cells, the transfection method includes one or more of viral and non-viral transfection methods, and the suicide gene is activated The time is one or more times. The drugs that initiate the suicide gene are acyclovir (ACV), ganciclovir (GCv), 5-fluorocytosine, 6-mercaptoxanthine, cyclophosphamide, 6 -Methylpurine-2-deoxyribonucleoside, ara-M, BVDU, CPA, IFO, CB1954, MeP-dR, F-araA, C...
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