M-gene based fluorescent RT-PCR (Reverse Transcription Polymerase Chain Reaction) detection method of Nipah virus

A RT-PCR, Nipah virus technology, applied in the field of inspection and quarantine, can solve the problems of no effective treatment and prevention, Nipah disease threat, etc., to achieve the effects of simple operation, enhanced specificity, and high specificity
CN102559935AInactive Publication Date: 2012-07-11ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2012-07-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses an M-gene based fluorescent RT-PCR (Reverse Transcription Polymerase Chain Reaction) detection method of Nipah virus, and a kit thereof. The method adopts a one-step fluorescent RT-PCR method to detect the Nipah virus in porcine samples, is simple, convenient and fast to operate, and has higher specificity and sensitivity. According to the method, false viruses without infectivity are adopted by the positive control sample, the property is stable, false viruses do not need to be cultured, and the laboratory biosafety is good.
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Description

technical field

[0001] The invention relates to a Nipah virus fluorescent RT-PCR detection method, in particular to a Nipah virus TaqMan-MGB probe fluorescent RT-PCR detection method, which belongs to the technical field of inspection and quarantine. Background technique

[0002] Nipah disease first broke out in Malaysia in 1998, causing morbidity and mortality in pigs and humans. Pathogen ecology and epidemiological surveys found that fruit-eating bats of the family Pteropus are the natural host of the pathogen of this disease, Nipah Virus (NiV), and play a decisive role in the process of spreading the disease to animals and humans. NiV is not pathogenic to bats, has certain pathogenicity to pigs, but is highly pathogenic to humans, and the fatality rate of humans after infection with NiV reaches 40% to 70%. Therefore, NiV is listed as the most dangerous biosafety level 4 pathogenic microorganism.

[0003] Together with Hendra virus, NiV belongs to the Henipavirus genus o...

Claims

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