Degradable composite material containing chitin or chitosan

A technology of composite materials and chitin, which is applied in the field of degradable composite materials, can solve the problems that the self-degradation ability of chitin and chitosan has not been established, and achieve storage stability, long-term storage stability, The effect of burden reduction

Inactive Publication Date: 2012-07-11
THERMOSTABLE ENZYME LAB +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, no technology capable of imparting such self-degrading ability to chitin and chitosan has been established

Method used

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  • Degradable composite material containing chitin or chitosan

Examples

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preparation example Construction

[0033] The chitin molded article can be produced by a known production method, or can be obtained as a commercial product.

[0034] The degrading enzyme used in the degradable composite material of the present invention is not particularly limited herein, as long as it can hydrolyze the polysaccharide and is pharmaceutically acceptable. Specific examples of such degrading enzymes include chitinase, chitosanase, and the like. For example, if the polysaccharide is chitin, chitinase is used, and if the polysaccharide is chitosan, chitosanase is used. The degrading enzyme used for the degradable composite material of the present invention may be one enzyme used alone, or two or more enzymes used in combination.

[0035] The effective temperature of the degrading enzyme used in the present invention is not particularly limited herein, as long as it exhibits activity at the temperature and environment at which the degradable composite material will be used, preferably the degrading...

Embodiment 1

[0052] 1. Preparation of Chitinase

[0053] Use LB medium (containing 10 g / L polypeptone, 5 g / L yeast extract, and 10 g / L sodium chloride) to shake culture at 37 °C on Escherichia coli carrying the plasmid, which has been introduced into the plasmid encoding DNA of the chitinase of Pyrococcus furiosus (amino acid sequence represented by SEQ ID NO: 2 in Japanese Patent Application Laid-Open No. 2004-344160), and in the logarithmic growth phase (optical density at 600 nm of 0.2 to 0.4 ) was added isopropyl β-thiogalactopyranoside (IPTG) so that the final concentration was 0.2 mM. Culturing was continued unchanged overnight, and then E. coli cells were collected by centrifugation (6,000 xg, 7 min).

[0054] E. coli cells recovered from 1 liter of culture were suspended in 20 mL of buffer A (25 mM Tris-HCl [pH 7.5], 1 mM EDTA, 25 mM NaCl) and lysed by sonication. After lysis, extracts were obtained by high-speed centrifugation (13,000 xg, 15 min). The extract was heated at 85...

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Abstract

Disclosed is a degradable composite material which has autolytic ability and contains at least one polysaccharide selected from among chitins and chitosans. By having a molded article, which contains at least one polysaccharide selected from among chitins and chitosans, support a degrading enzyme that is capable of hydrolyzing the polysaccharide, it becomes possible to gradually degrade the polysaccharide in an environment where moisture is present, such as a living organism.

Description

technical field [0001] The present invention relates to a degradable composite material containing at least one polysaccharide selected from chitin and chitosan. More specifically, the present invention relates to a degradable composite material which contains at least one polysaccharide selected from chitin and chitosan and is slowly degraded in an environment where moisture exists, such as a living body. Background technique [0002] Since chitin and chitosan do not adversely affect the ecosystem, they are used in various medical materials (see Patent Document 1). For example, sheets prepared using chitin and chitosan as raw materials are used as wound dressing materials, and fibers prepared using chitin and chitosan as raw materials are used as medical repair materials. It is known that when chitin and chitosan are degraded, they become soluble sugars that are absorbed in the body, and their degradation products are known to be highly biocompatible. However, there are s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/00A61L15/64
CPCA61L26/0023A61L15/28A61L27/227A61L15/38A61L27/58A61L27/20A61L26/009A61L15/64A61L26/0047A61P17/02C08L5/08
Inventor 奥崇野口武
Owner THERMOSTABLE ENZYME LAB
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