Application of bauhinia championii general flavone to preparation of medicament for treating rheumatoid arthritis
A rheumatoid, total flavonoid technology, applied in the directions of drug combination, antipyretic, anti-inflammatory agent, etc., to achieve the effects of clear efficacy, controllable quality and stable preparation method
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[0018] The extraction of the total flavonoids of the vine of embodiment 1
[0019] 1.1 Reflux extraction method Weigh 20g of asparagus coarse powder, add 200ml of 70% ethanol to soak for 30min, then heat and reflux on a water bath to extract twice. Each time for 1h, filter while hot, and wash the residue with the corresponding solvent for 2-3 times , the combined filtrate was recovered until there was no alcohol smell, dissolved in water to contain 0.5g crude drug per 1mL, centrifuged (4000r / min) for 15min, filtered, and set aside. Take 1.0mL and put it in a 25mL measuring bottle, add 6.0mL of water; add 1.0mL of 5% (mass fraction) sodium nitrite solution, mix well, and let stand for 6min; add 10% (mass fraction) aluminum nitrate 1.0mL, shake Mix well and let stand for 6 minutes; then add 10.0 mL of 4% (mass fraction) NaOH solution, add water to the mark, shake well, let stand for 15 minutes; measure the absorbance A at a wavelength of 500 nm according to spectrophotometry (Ap...
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