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Method for preparing phosphomycin calcium/hexagonal mesoporous silicon/poly glycolide-co-lactide (PLGA) bone tissue stent

A technology of hexagonal mesoporous silicon and calcium phosphate is applied in the field of preparation of biomedical materials to achieve the effects of being beneficial to cell adhesion and proliferation, low cost of raw materials and high strength

Active Publication Date: 2014-02-26
广州智园生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the drug loading performance of hexagonal mesoporous silicon is excellent, there are still defects of burst release

Method used

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  • Method for preparing phosphomycin calcium/hexagonal mesoporous silicon/poly glycolide-co-lactide (PLGA) bone tissue stent
  • Method for preparing phosphomycin calcium/hexagonal mesoporous silicon/poly glycolide-co-lactide (PLGA) bone tissue stent
  • Method for preparing phosphomycin calcium/hexagonal mesoporous silicon/poly glycolide-co-lactide (PLGA) bone tissue stent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Dissolve 0.8g of PLGA in 10mL of dichloromethane to obtain a PLGA solution; mix 0.3g of calcium phosphate salt particles with 0.3g of hexagonal mesoporous silicon to obtain a calcium phosphate / hexagonal mesoporous silicon blend; mix 0.6g of phosphoric acid Calcium salt / hexagonal mesoporous silicon blend was mixed with 10mL PLGA solution evenly to obtain 10mL calcium phosphate / hexagonal mesoporous silicon / PLGA mixed solution; Stir continuously at 600r / min for 17h, wash with deionized water and freeze-dry for 24h to obtain calcium phosphate / hexagonal mesoporous silicon / PLGA microspheres; fill the microspheres into a cylindrical mold, set the temperature at 95°C for 4h, and cool After reaching room temperature, demould, the calcium phosphate salt / hexagonal mesoporous silicon / PLGA bone tissue scaffold is prepared.

Embodiment 2

[0030] Dissolve 0.5g of PLGA in 10mL of dichloromethane to obtain a PLGA solution; mix 0.2g of calcium phosphate salt particles with 0.4g of hexagonal mesoporous silicon to obtain a calcium phosphate / hexagonal mesoporous silicon blend; mix 0.6g of phosphoric acid The calcium salt / hexagonal mesoporous silicon blend was mixed evenly with 10mL PLGA solution to obtain 10mL calcium phosphate / hexagonal mesoporous silicon / PLGA mixed solution; Stir continuously at 280r / min for 5h, wash with deionized water and freeze-dry for 48h to obtain calcium phosphate / hexagonal mesoporous silicon / PLGA microspheres; fill the microspheres into a cylindrical mold, set the temperature at 80°C for 8h, and cool to room temperature, demolded, and prepared calcium phosphate / hexagonal mesoporous silicon / PLGA

Embodiment 3

[0032] Dissolve 1.0g of PLGA in 10mL of dichloromethane to obtain a PLGA solution; mix 0.3g of calcium phosphate salt particles with 0.1g of hexagonal mesoporous silicon to obtain a calcium phosphate / hexagonal mesoporous silicon blend; mix 0.4g of phosphoric acid Mix the calcium salt / hexagonal mesoporous silicon blend with 10mL PLGA solution evenly to obtain 10mL calcium phosphate / hexagonal mesoporous silicon / PLGA mixed solution; add the mixed solution to 500mL 7% polyvinyl alcohol aqueous solution, room temperature 150r / min and continuously stirred for 20 hours, washed with deionized water and freeze-dried for 72 hours to obtain calcium phosphate / hexagonal mesoporous silicon / PLGA microspheres; fill the microspheres into a cylindrical mold, set the temperature at 130°C for 6 hours, and cool to At room temperature, the mold was removed to prepare the calcium phosphate / hexagonal mesoporous silicon / PLGA bone tissue scaffold.

[0033]Table 1 shows the pore parameters of the calci...

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Abstract

The invention discloses a method for preparing a phosphomycin calcium / hexagonal mesoporous silicon / poly glycolide-co-lactide (PLGA) bone tissue stent. The method comprises the following steps of: mixing a dichloromethane solution of PLGA and phosphomycin calcium / hexagonal mesoporous silicon comixture uniformly to obtain a phosphomycin calcium / hexagonal mesoporous silicon / PLGA mixed solution; adding the mixed solution into a polyvinyl alcohol aqueous solution, stirring at room temperature, washing by using water, cooling and drying to obtain composite microspheres; filling the microspheres into a cylindrical die, keeping temperature for sizing, cooling to room temperature, and demolding to obtain the phosphomycin calcium / hexagonal mesoporous silicon / PLGA bone tissue stent. According to the preparation method, a technology is simple and feasible; the prepared bone tissue stent has high biocompatibility and degradability and a three-dimensional connected porous structure, and is uniform in pore structure, high in strength and porosity and large in space; the surface of the stent has certain roughness, so that the adhesive multiplication of cells is facilitated; and the bone tissue stent can be used for repairing and reconstructing defective bone tissues.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and relates to a preparation method of a bone tissue support for bone tissue engineering repair, in particular to a preparation method of calcium phosphate / hexagonal mesoporous silicon / PLGA bone tissue support. Background technique [0002] Bone tissue engineering refers to the cultivation and expansion of isolated autologous high-concentration osteoblasts, bone marrow stromal stem cells or chondrocytes in a natural or synthetic biocompatibility that can be gradually degraded by the human body. Absorbed cell scaffolds or extracellular matrix technology. The role of tissue engineering scaffolds in tissue engineering is mainly as a carrier of cells and biological factors and providing support for new tissues. It not only physically connects and supports cells and tissues, but also regulates various functional activities of cells. This biomaterial scaffold can provide ce...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/12A61L27/02A61L27/56
Inventor 魏坤许为康
Owner 广州智园生物科技有限公司
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