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Method for preparing hydroxyapatite/poly glycolide-co-lactide (PLGA)/chitosan three-dimensional porous stent

A hydroxyapatite, three-dimensional porous technology, applied in the field of preparation of biomedical materials, can solve the problems of increasing the size of scaffold materials, and achieve the effects of industrialization, easy availability, and high porosity

Inactive Publication Date: 2012-07-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the range of bone defects in these currently studied animal models is basically small, and larger clinical bone defects require an increase in the size of the scaffold material

Method used

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  • Method for preparing hydroxyapatite/poly glycolide-co-lactide (PLGA)/chitosan three-dimensional porous stent
  • Method for preparing hydroxyapatite/poly glycolide-co-lactide (PLGA)/chitosan three-dimensional porous stent
  • Method for preparing hydroxyapatite/poly glycolide-co-lactide (PLGA)/chitosan three-dimensional porous stent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Dissolve 0.8g PLGA in 10mL dichloromethane to obtain 10mL PLGA solution; mix 0.5g hydroxyapatite powder with 10mL PLGA solution evenly to obtain 10mL hydroxyapatite / PLGA mixed solution; dissolve 0.1g chitosan in In 10mL 3% dilute acetic acid aqueous solution, obtain 10mL chitosan solution; Add chitosan solution in 100mL 2% polyvinyl alcohol aqueous solution, obtain 110mL chitosan / polyvinyl alcohol solution; Hydroxyapatite / The PLGA mixture was added to the chitosan / polyvinyl alcohol aqueous solution, stirred continuously at room temperature at 150 r / min for 20 h, washed with deionized water and freeze-dried for 24 h to obtain hydroxyapatite / PLGA / chitosan microspheres. Fill the microspheres into a cylindrical mold, set the temperature at 130°C and keep it warm for 6 hours, cool to room temperature, and demould to prepare a three-dimensional porous bone scaffold for tissue engineering of hydroxyapatite / PLGA / chitosan.

Embodiment 2

[0030] Dissolve 2.0 g of PLGA in 10 mL of dichloromethane to obtain 10 mL of PLGA solution; mix 2.0 g of hydroxyapatite powder with 10 mL of PLGA solution to obtain 10 mL of hydroxyapatite / PLGA mixture; dissolve 1.0 g of chitosan in In 10mL 1% dilute acetic acid aqueous solution, obtain 10mL chitosan solution; Add chitosan solution in 100mL 10% polyvinyl alcohol aqueous solution, obtain 110mL chitosan / polyvinyl alcohol solution; Hydroxyapatite / The PLGA mixture was added to the chitosan / polyvinyl alcohol aqueous solution, stirred continuously at 600 r / min at room temperature for 10 h, washed with deionized water and freeze-dried for 72 h to obtain hydroxyapatite / PLGA / chitosan microspheres. Fill the microspheres into a cylindrical mold, set the temperature at 105°C and keep it warm for 10 hours, cool to room temperature, and demould to prepare a three-dimensional porous bone scaffold for tissue engineering of hydroxyapatite / PLGA / chitosan.

Embodiment 3

[0032]Dissolve 0.5g PLGA in 10mL dichloromethane to obtain 10mL PLGA solution; mix 0.6g hydroxyapatite powder with 10mL PLGA solution evenly to obtain 10mL hydroxyapatite / PLGA mixed solution; dissolve 0.3g chitosan in In 10mL 2% dilute acetic acid aqueous solution, obtain 10mL chitosan solution; Add chitosan solution in 100mL 5% polyvinyl alcohol aqueous solution, obtain 110mL chitosan / polyvinyl alcohol solution; Hydroxyapatite / The PLGA mixture was added to the chitosan / polyvinyl alcohol aqueous solution, stirred continuously at room temperature at 270r / min for 5h, washed with deionized water and freeze-dried for 48h to obtain hydroxyapatite / PLGA / chitosan microspheres. Fill the microspheres into a cylindrical mold, set the temperature at 90° C. for 7 hours, cool to room temperature, and demould to prepare a three-dimensional porous scaffold for tissue engineering of hydroxyapatite / PLGA / chitosan.

[0033] Table 1 shows the pore parameters of the hydroxyapatite / PLGA / chitosan th...

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Abstract

The invention discloses a method for preparing a hydroxyapatite / poly glycolide-co-lactide (PLGA) / chitosan three-dimensional porous stent. The method comprises the following steps of: mixing a dichloromethane solution of PLGA and hydroxyapatite uniformly to obtain a hydroxyapatite / PLGA mixed solution; dissolving chitosan in an acetic acid aqueous solution, and adding the mixture into a polyvinyl alcohol aqueous solution to obtain a chitosan / polyvinyl alcohol solution; adding the hydroxyapatite / PLGA mixed solution into the chitosan / polyvinyl alcohol solution, stirring at room temperature, washing by using water, cooling and drying to obtain composite microspheres; and filling the microspheres into a die, keeping temperature for sizing, cooling to room temperature, and demolding to obtain the hydroxyapatite / PLGA / chitosan three-dimensional porous stent. The three-dimensional porous stent has high biocompatibility and degradability and a three-dimensional connected porous structure, and is uniform in pore structure and high in strength and porosity; the surface of the three-dimensional porous stent has certain roughness, so that the adhesive multiplication of cells is facilitated; and the three-dimensional porous stent can be used for repairing and reconstructing defective bone tissues.

Description

technical field [0001] The invention belongs to the technical field of preparation of biomedical materials, and relates to a preparation method of a three-dimensional porous scaffold for bone tissue engineering repair, in particular to a preparation method of a hydroxyapatite / PLGA / chitosan three-dimensional porous scaffold. Background technique [0002] Bone tissue engineering refers to the cultivation and expansion of isolated autologous high-concentration osteoblasts, bone marrow stromal stem cells or chondrocytes in a natural or synthetic biocompatibility that can be gradually degraded by the human body. Absorbed cell scaffolds or extracellular matrix technology. The role of tissue engineering scaffolds in tissue engineering is mainly as a carrier of cells and biological factors and providing support for new tissues. It not only physically connects and supports cells and tissues, but also regulates various functional activities of cells. This biomaterial scaffold can pr...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/20A61L27/18A61L27/12
Inventor 魏坤许为康
Owner SOUTH CHINA UNIV OF TECH