Production method for medical grade valine

Abstract

The invention discloses a production method for high purity medical grade valine. Industrial valine raw material is prepared to be water solution higher than 5 percent under the temperature of 50 to 65 DEG C, flocculating agent is added, modified activated carbon is added after filtering, ultrafiltration is performed by using an ultra filtration membrane with molecular weight cutoff larger than 500, then vacuum concentration and cooling crystallization are performed to obtain the crystal product of medical grade valine, mother solution is added into a gel chromatography column for chromatographic separation, pure water with the temperature of 40 to 50 DEG C is taken as eluate, valine solution flowing out of the column is collected, after concentrated crystallization, the crystal product of medical grade valine is obtained, and finally, the removing rate of bacterial endotoxin and protein in the product can reach 99 percent, lactamic acid, aminocarproic acid and isoleucine can be effectively removed, the purity of the product can reach 99 percent, medical grade standards are met, the comprehensive yield of products can reach more than 96 percent, and large-scale production can be achieved.

Description

technical field [0001] The invention relates to a method for separating and purifying amino acids, in particular to a method for producing high-purity pharmaceutical-grade L-valine. Background technique [0002] L-valine is a branched amino acid, which is one of the essential amino acids for the human body. It has a variety of physiological functions and is widely used in the fields of medicine, food and seasoning, animal feed and cosmetics. In the field of medicine, valine is often used in the manufacture of compound amino acid infusion and amino acid oral liquid to treat blood-brain barrier, hepatic coma, chronic liver cirrhosis and renal failure, congenital metabolic defects, sepsis and diabetes, etc., and can also be used to speed up surgical trauma. Medicine for healing. [0003] The main production method of valine is the fermentation method, using glucose as a nutrient, and producing valine after corresponding enzyme fermentation. During the fermentation process, be...

AI Technical Summary

Problems solved by technology

[0007] The extraction of food-grade and pharmaceutical-grade valine generally adopts the ion exchange method, but this method has the following problems: First, the bacteria are removed by filtration. Because the bacteria are relatively small, the removal of the bacteria is usually not complete; The second is to use a large amount of activated carbon when removing pigment and bacterial endotoxin impurities, and requires multiple treatments. The process is relatively complicated and the pollution is relatively large. At the same time, activated carbon also adsorbs more valine, which reduces the yield.

The third is that there are many impurities in valine, and the content is high. The fourth is that the resin is seriously polluted, which is easy to cause damage to the resin structure and lose the regeneration ability, which increases the operating cost. Regeneration times, causing problems such as sewage discharge

[0008] CN101721979A discloses a special macroporous adsorption resin for valine separation. Valine, alanine and leucine can be separated by using the resin, and the product purity can reach more than 99%, but the production of the resin is still in the experimental stage. Cannot be used in industrial production on a large scale

[0009] In addition, the Chinese patent application publication CN101798273A uses heating to 220±10°C to sublimate and remove alanine and isoleucine, but valine is easily decomposed and destroyed at high temperature

Images