Method for extracting and separating tectoridin from iris tectorum

Abstract

The invention discloses a method for extracting and separating tectoridin from iris tectorum. The method comprises the following steps of: (1) taking iris tectorum, drying, crushing, and sieving through a 60-100 mesh sieve; (2) weighting the sieved iris tectorum, adding into a 15-20mL / g two-phase solvent system with a volume ratio of normal butanol to water being (1:2)-(2:1), stirring and extracting, wherein the extracting time is 1.5-5h, the stirring speed is 280-800rpm and the extracting temperature is at 25-70 DEG C; and (3) after layering and stabilizing a filtrate obtained after filtering in a liquid-separating funnel, separating a normal butanol phase, reducing pressure and concentrating to 40-50mL, overnight aging till forming a large number of yellowish white educt, filtering, washing the filter residue with less absolute ethyl alcohol, and drying so as to obtain a white tectoridin crude product, wherein the content of the tectoridin in the crude product is not less than 74%; and twice re-crystallizing by using an organic solvent, thereby obtaining a tectoridin product. The method has the advantages of simple steps, low cost, and high purity of obtained product, and the content of tectoridin after re-crystallizing is above 93%.

Description

technical field

[0001] The invention relates to a method for extracting plant effective components, in particular to a method for extracting and separating Shegan glycosides from Sichuan Shegan. Background technique

[0002] Chuanshegan is the dried rhizome of Iris tectorum Maxim., which is a plant of the genus Iris in the family Iridaceae. It is mainly produced in Guangzhou, Guangxi, Sichuan, Guizhou and other places in my country. It is mainly used to treat sore throat, phlegm, cough and asthma, etc. It was officially included in the 2005 edition of Pharmacopoeia of the People's Republic of China.

[0003] Studies have proved that the main active ingredients in Sichuan Shegan are irisin (also known as Shegan glycoside), wild irisin, irisin, wild irisaxanthin, and second wild irisaxanthin and other isoflavones, which have anti-inflammatory and antiviral properties. , detoxification and other pharmacological activities. Existing experimental studies have shown that irisin a...