Method for refining capsanthin by enzyme catalysis, esterification and molecular distillation

A technology of capsanthin and molecular distillation, which is applied in the field of natural organic chemistry, can solve the problems of by-products that cannot be directly used, difficult degassing, high distillation temperature, etc., and achieves the effects of low production cost, simple process and high product content

Active Publication Date: 2012-08-01
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process: (1) After degumming with low-carbon alcohol, it does not need to carry out solvent recovery, but directly participates in the esterification reaction; (2

Method used

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  • Method for refining capsanthin by enzyme catalysis, esterification and molecular distillation
  • Method for refining capsanthin by enzyme catalysis, esterification and molecular distillation

Examples

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Example Embodiment

[0031] Example 1

[0032] The present invention is an enzyme-catalyzed esterification molecular distillation method for refining capsanthin (such as figure 1 Shown), including the following steps:

[0033] Step 1: Degumming

[0034] (1) Put 10 kg of capsicum red oleoresin and 10 kg of degumming agent (absolute ethanol) into a jacketed reactor, and stir at 60°C at a speed of 100 revolutions per minute.

[0035] (2) After stirring evenly, pump the mixed solution into the freezing tank, control the temperature of the freezing tank to 5°C, and let it settle down.

[0036] (3) After 5 hours of sedimentation, the vacuum degree of the freezing liquid is 3×10 4 Under Pa conditions, the upper part of the freezing tank is sucked into the vacuum filter to obtain the filtrate, and the filtrate is separated by the tubular centrifuge to obtain the degummed pigment solution.

[0037] Step 2: Esterification

[0038] (1) Add the degummed pigment solution to the enzyme reactor, and add 50 g of lipase (Nov...

Example Embodiment

[0043] Example 2

[0044] In this embodiment, an enzyme-catalyzed esterification molecular distillation method for refining capsanthin, except for the following differences, other steps and process conditions are the same as in embodiment 1:

[0045] (1) Add capsicum red oleoresin and methanol with masses of 10Kg and 50Kg, respectively, and stir evenly at a speed of 500 revolutions / min at 20°C.

[0046] (2) The temperature of the freezing tank is 3°C, and it is allowed to settle for 10 hours.

[0047] (3) The esterification reaction temperature is 45° C., the reaction is 5 hours, and the stirring speed is 150 rpm.

[0048] (4) Dehydration under reduced pressure for 2 hours.

[0049] (5) Decompression recovery solvent temperature is 40℃, vacuum degree is 6×10 4 Pa.

[0050] (6) The mass ratio of degumming agent, lipase and water is 100:3:3.

[0051] (7) Lipase is Lipozyme RM IM.

[0052] (8) The first-stage degassing temperature is 100℃, and the vacuum degree is 8×10 3 Pa, secondary distilla...

Example Embodiment

[0055] Example 3

[0056] In this embodiment, an enzyme-catalyzed esterification molecular distillation method for refining capsanthin, except for the following differences, other steps and process conditions are the same as in embodiment 1:

[0057] (1) Add capsicum red oleoresin and the mass fraction of 95% ethanol aqueous solution with masses of 10Kg and 100Kg, respectively, and stir uniformly at a speed of 800 rpm at 35°C.

[0058] (2) The temperature of the freezing tank is 0°C, and it is allowed to stand and settle for 20 hours.

[0059] (3) The esterification reaction temperature is 30°C, the reaction is for 10 hours, and the stirring speed is 300 rpm.

[0060] (4) Dehydration under reduced pressure for 3 hours.

[0061] (5) Decompression recovery solvent temperature is 50℃, vacuum degree is 4×10 4 Pa.

[0062] (6) The mass ratio of degumming agent, lipase and water is 100:10:5.

[0063] (7) Lipase is Lipozyme RM IM.

[0064] (8) The first-stage degassing temperature is 50℃, and the ...

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Abstract

The invention discloses a method for refining capsanthin by enzyme catalysis, esterification and molecular distillation. The method comprises the following steps of: mixing chili red oil resin and a degumming agent in a mass ratio of 1:1-1:10, stirring uniformly at the temperature of between 20 and 60 DEG C and at the speed of 100 to 800 revolutions per minute, and standing or intermittently stirring and settling at the temperature of between 0 and 5 DEG C; after settling for 5 to 20 hours, filtering under a vacuum condition to obtain filtrate, and separating the filtrate to obtain a degummedpigment solution; adding lipase and water into the degummed pigment solution, mixing uniformly, and reacting at the temperature of between 30 and 60 DEG C under stirring at the speed of 50 to 300 revolutions per minute; after reacting for 2 to 10 hours, dehydrating for 1 to 3 hours; filtering out the lipase, and reclaiming a solvent from the pigment solution under reduced pressure to obtain a fatty acid ester solution containing the capsanthin; and performing two-stage molecular distillation on the fatty acid ester solution to obtain the capsanthin with high color value. The method has the advantages of simple process, high product content, few insoluble acetone substances and low production cost, and is suitable for industrialized production.

Description

technical field [0001] The invention relates to a production process of high color-value capsanthin, in particular to a method for producing high-color-value capsanthin by utilizing enzyme-catalyzed esterification and molecular distillation. The invention belongs to the field of natural organic chemistry. Background technique [0002] Capsanthin is a high-quality natural carotenoid food coloring. Its main components are capsanthin, capsanthin and capsanthin. It not only has bright color, high color value, strong tinting strength, but also has high safety , has no toxic side effects on the human body, and can prolong the shelf life of simulated food, and is widely used in industries closely related to human health such as food, medicine and cosmetics. [0003] Domestically produced capsanthin is basically extracted with 6# solvent, n-hexane, ethanol and other solvents. The capsanthin produced after concentration and recovery of solvent is a kind of oleoresin, which contains ...

Claims

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Application Information

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IPC IPC(8): C09B61/00C09B67/10C09B67/54C12P7/62
Inventor 吕扬效金伟铮
Owner SOUTH CHINA UNIV OF TECH
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