Method for preparing spring dextrin by aid of alpha amylase for hydrolyzing amylose starch

An amylose and alpha amylase technology, which is applied in the directions of medical preparations, applications, and pharmaceutical formulations of non-active ingredients to achieve mild conditions, fewer side reactions, and narrow distribution of product components.

Inactive Publication Date: 2013-10-30
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, amylose is insoluble in water, so that amylose has certain limitations in the use of embedding

Method used

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  • Method for preparing spring dextrin by aid of alpha amylase for hydrolyzing amylose starch
  • Method for preparing spring dextrin by aid of alpha amylase for hydrolyzing amylose starch

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of spring dextrin

[0018] Dissolve 5 parts of 1g amylose into 10mL NaOH solution (90℃, 0.5mol / L), add 1L distilled water, adjust the temperature to 60℃, and adjust pH 6.0 with 2mol / L HCl. Then add 10U enzyme to each amylose solution, keep it at 60℃ for 1h, 2h, 3h, 4h, 5h, and finally add 5mol / L HCl solution to adjust the pH to below 3. The powder product of spring dextrin is obtained by spray drying.

Embodiment 2

[0019] Example 2 Color development of spring dextrin and iodine solution

[0020] Dissolve the spring dextrin prepared in Example 1 in distilled water, add iodine solution to develop color, and perform full-wavelength scanning, using the formula (1 / λ max =1.558×10 -3 +102.5×10 -4 (1 / )) can calculate the average degree of polymerization of spring dextrin. Spring dextrin hydrolyzed for 1h, 2h, 3h, 4h, 5h They are 36.6, 25.9, 14.7, 12.5 and 11.7 respectively.

Embodiment 3

[0021] Example 3 Detection of the molecular weight distribution of spring dextrin by high performance anion chromatography

[0022] The powdered spring dextrin in Example 1 was detected by high performance anion chromatography. The detection conditions were Dionex ICS-500 chromatography system. The column model used was Dionex CarboPAC PA200 (250×4 mm ID); the detection mobile phase was 100mM sodium hydroxide Solution (eluent A), 100mM sodium hydroxide solution containing 600mM sodium acetate (eluent B); the elution gradient is 20% at 0 minutes, eluent B, and at 60 minutes, eluent B is 100%; The flow rate is 1 mL / min, and the sample load is 25 μL. The high-efficiency anion chromatogram of the product spring dextrin after different hydrolysis time is as follows figure 2 Shown.

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Abstract

The invention provides a method for preparing spring dextrin by the aid of alpha amylase for hydrolyzing amylose starch, which belongs to the field of food and drug microcapsule embedding materials. The method includes the steps: firstly, dissolving the amylose starch in aqueous alkali, adding a certain amount of water and then adjusting pH (potential of hydrogen) to the most proper hydrolysis range of the alpha amylase; secondly, adding the alpha amylase and degenerating and inactivating enzyme by means of acidification after performing enzymolysis for a certain time; and finally, obtaining the spring dextrin with different average molecular weights by means of drying. The method has the advantages of simple process, mild conditions, narrow product component distribution, less side reaction and no need of desalination. By the aid of the method, the spring dextrin with different average polymerization sizes can be prepared conveniently.

Description

Technical field [0001] A method for preparing spring dextrin by hydrolyzing amylose with alpha amylase. The invention relates to the field of food and medicine microcapsule embedding materials. Background technique [0002] In recent years, various decomposition products of starch have been used in various food systems as a food additive. Various types of dextrins prepared by hydrolysis of various starches by acids or enzymes are used to protect nutritional fortification factors and prepare special functional sports drinks. Of course, there are many applications in the field of medicine as a carrier for targeted drug delivery. [0003] Spring dextrin is a series of dextrins with different lengths obtained by hydrolyzing amylose by alpha amylase or hydrolyzing starch by debranching enzyme. Because its spatial structure is similar to the shape of a spring, and its spiral structure has a certain degree of reversibility and flexibility, it is called spring dextrin. Due to its short c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14A23P1/04A61K47/36A61K9/50A23P10/30
Inventor 徐学明徐进赵文秀杨哪金征宇
Owner JIANGNAN UNIV
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