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Production method of isomaltooligosacharide and animal feed additive

A technology of malt syrup and cassava, applied in animal feed, animal feed, application, etc., to reduce production costs, improve sticking phenomenon, and increase the final yield of products

Active Publication Date: 2013-05-29
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has a good effect, isomaltooligosaccharide is still not widely used in the feed industry. The main reason is that the price of isomaltooligosaccharide sold in the market is relatively high. Adding it to feed will lead to increased feed cost and increased breeding. cost

Method used

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  • Production method of isomaltooligosacharide and animal feed additive
  • Production method of isomaltooligosacharide and animal feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: The relationship between the particle size of starch milk in cassava homogenate and the yield of filtrate, filter cake and isomaltooligosaccharide after saccharification

[0041] Immediately after harvesting, fresh cassava is peeled and cut into pieces of about 1cm 2 The cassava grains were pulverized for 1 min with a high-speed universal pulverizer. The crushed cassava homogenate has a Baume degree of 28, and it is placed in an oven at 105°C for 4 hours. After drying until the weight does not change, its moisture content is measured to be 65%, so the cassava homogenate contains 35% dry matter. The dried powder was hydrolyzed with 6N hydrochloric acid for 2 hours, and the DE value was determined by the method of Fihring's reagent to be 95.5%, that is, the starch content in the fresh cassava was 33.42% based on glucose.

[0042] Four kinds of cassava homogenate samples passed through different mesh sieves of 10, 40, 60, and 100 mesh were prepared respective...

Embodiment 2

[0049] (1) Cut the peeled 1cm 2 Fresh cassava grains are mechanically crushed twice to pass through a 100-mesh sieve to obtain cassava homogenate;

[0050] (2) Gradually add 50 g of compound enzyme preparation into 1000 g of cassava homogenate with a pH of 5.3, react at 55°C for 0.5 h, and keep stirring during the process. After the reaction, adjust the pH of the system to 6.0 and add 0.10 mL of high-temperature α-amylase (40000 U / mL), stir and liquefy in a boiling state for 2 hours, after the reaction is completed, lower it to normal temperature, add hydrochloric acid to adjust the pH to 3.5 to inactivate the enzyme, and obtain a liquefied solution ;

[0051] (3) Adjust the pH value of the liquefaction solution to 5.8, add 0.1g Amano fungal amylase (15000U / g) at 60°C, add 0.1mL transglucosidase (300000U / mL) after reacting for 8 hours, and continue at 60°C After reacting for 45 hours, the saccharification solution was obtained after the enzyme was inactivated, which was ordi...

Embodiment 3

[0061] (1) Divide 1cm 2 Fresh cassava grains are mechanically crushed twice to pass through a 100-mesh sieve to obtain cassava homogenate.

[0062] (2) Gradually add 0.01 g of compound enzyme preparation into 1000 g of cassava homogenate with a pH of 5.5, react at 60° C. for 10 h, and keep stirring during the process. After the reaction, adjust the pH of the system to 6.0 and add 10mL of high-temperature α-amylase (40000U / mL), stir and liquefy in boiling state for 0.5h, after the reaction is completed, lower it to normal temperature, add hydrochloric acid to adjust the pH to 3.5 to inactivate the enzyme, and obtain a liquefied solution ;

[0063] (3) Adjust the pH value of the liquefaction solution to 5.8, add 10g Amano fungal amylase (15000U / g) at 60°C, add 10mL transglucosidase (300000 U / mL) after reacting for 0.5h, and continue at 60°C After reacting for 5 hours, the temperature was raised to 75°C to inactivate the enzyme for 10 minutes to obtain a saccharification soluti...

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Abstract

The invention relates to the technical field of isomaltooligosacharide and especially relates to a production method of isomaltooligosacharide. The production method of isomaltooligosacharide comprises the following steps of crushing cassava to obtain uniform cassava pulp, adding a complex enzyme preparation into the uniform cassava pulp, adding high-temperature alpha-amylase into the mixture to obtain a liquefaction liquid, treating the liquefaction liquid orderly through fungal amylase and transglucosidase to obtain a saccharification liquid which is common isomaltooligosacharide 500, diluting 1kg of the saccharification liquid until dry matter content is in a range of 8 to 10wt%, adding 0.3 to 0.6g of active dry yeast into the diluted saccharification liquid to obtain a fermentation broth which is isomaltooligosacharide 900, and mixing the fermentation broth and the liquefaction liquid according to a weight ratio of (3 to 4): 1 to obtain isomaltooligosacharide 500 which does not contain glucose, wherein the total content of isomaltose, panose and isomaltotriose is great than or equal to 35%. The invention also relates to an animal feed additive. The animal feed additive is prepared by adding corn protein powder into different types of isomaltooligosacharide syrup obtained by the production method and drying the mixture.

Description

technical field [0001] The invention relates to the technical field of isomaltooligosaccharides, in particular to a production method of isomaltooligosaccharides, and also relates to an animal feed additive containing the isomaltooligosaccharides. Background technique [0002] Isomaltooligosaccharide (IMO for short) refers to a class of oligosaccharides in which the number of monosaccharides ranging from 2 to 6 is formed by the combination of glucose groups with α-1,6 glycosidic bonds. sugar, isomaltotriose and isomaltotetraose, etc. Isomaltooligosaccharide has physiological functions such as promoting the growth and reproduction of beneficial bacteria in the intestinal tract—Bifidobacteria and preventing dental caries. It is a functional oligosaccharide that combines nutrition and health care. The industrial production of isomaltooligosaccharide generally uses corn or potato starch as raw materials, such as rice starch, corn starch, tapioca starch, etc. Since 2009, star...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14A23K1/14A23K1/16
Inventor 刘宗利王乃强张姝王彩梅李方华
Owner BAOLINGBAO BIOLOGY
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