Application of tamoxifen, rapamycin and ginsenoside Rg3 composition in preparing medicaments for treating liver cancer

A technology of tamoxifen and ginsenoside, which is applied in drug combinations, antineoplastic drugs, and pharmaceutical formulations, can solve the problems of high recurrence rate, little curative effect enhancement, slow postoperative healing, etc., to reduce toxic and side effects, Significant curative effect, the effect of inhibiting the formation of tumor blood vessels

Inactive Publication Date: 2013-04-17
JIANGSU PROVINCE HOSPITAL
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  • Abstract
  • Description
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Problems solved by technology

[0006]Current literature reports generally use tamoxifen or the combination of tamoxifen and rapamycin in clinical application, but actual clinical experience shows that patients are using the above-mentioned However, it is still found that the curative effect is not very synergistic, especially for patients with surgically resected liver cancer, the recurrence rate is high, and the postoperative healing is slow

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  • Application of tamoxifen, rapamycin and ginsenoside Rg3 composition in preparing medicaments for treating liver cancer

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Experimental program
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Effect test

Embodiment 1

[0016] 1. Reagents: RPMI-1640 medium is a product of GIBCOL BRL Company in the United States; mouse anti-survivin and p70s6k Thr389 monoclonal antibodies were purchased from Cell Signal Company; secondary antibody HRP-labeled anti-mouse IgG and chemiluminescence (ECL) substrate were purchased from Santa Cruz Company; tamoxifen, rapamycin, and ginsenoside Rg3 were all purchased from Sigma Company, and Renilla luciferase plasmid pRL-CMV was purchased from Promega Company. Plasmid pLUC encoding firefly luciferase gene and survivin promoter sequence was donated by Dr. Xun from University of North Carolina, USA.

[0017] 2. Cell culture: The human liver cancer HepG2 cell line was routinely cultured in RPMI-1640 culture medium, adding 10% calf serum, human penicillin (concentration 100U / ml) and streptomycin (concentration 100 μg / ml), 37°C, 5%CO 2 cultured in an incubator. After 3-4 generations of adherent growth in the incubator, the cells in the logarithmic growth phase were take...

Embodiment 2

[0029] Tamoxifen has achieved obvious curative effect in inhibiting tumor growth in breast cancer and liver cancer experiments, and tamoxifen combined with rapamycin and ginsenoside Rg3 can enhance the effect of its cytotoxic drugs. Therefore, the combined therapy of tamoxifen combined with rapamycin and ginsenoside Rg3 will provide an efficient, low-toxic and cheap means for the treatment of liver cancer.

[0030] We have carried out clinical detection of survivin, p-Akt, and ER expression in tissue samples after liver cancer surgery in more than 100 cases. For patients with positive expression of survivin and p-Akt, moxifen combined with rapamycin and ginsenoside were partly used during outpatient follow-up. Rg3, tamoxifen monotherapy and control observation and follow-up, at least 2 courses of treatment. The results showed that: compared with the monotherapy group and the observation group (the control group was not administered), the three-drug combination therapy group (m...

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Abstract

The invention relates to application of tamoxifen combined with rapamycin and ginsenoside Rg3 in preparing medicaments for treating liver cancer. When the tamoxifen is combined with the rapamycin and the ginsenoside Rg3 to treat the liver cancer, sensibility of liver cancer cells on chemotherapy can be improved. The tamoxifen inhibits activeness of an Akt to induce apoptosis of mammary epithelialcells. When an Akt access is activated, breast cancer cells are induced to generate drug resistance to the tamoxifen. The tamoxifen, the rapamycin and the ginsenoside can be used jointly to induce the apoptosis of tumor cells in a cooperation mode. Besides, the ginsenoside Rg3 can inhibit expression of vascular endothelial growth factors (VEGF) of the tumor cells to inhibit the formation of tumorvessels, further can inhibit expression of matrix metalloproteinases and interfere interaction of endothelial cells and extracellular matrixes to block infiltration and metastasis of tumors, improve thoroughness of operation treatment and reduce the rate of recrudescence and metastasis after an operation, and can improve self immunity of a tumor patient, inhibit tumor metastasis and reduce toxic and side effects of the tumor patient after the chemoradiotherapy.

Description

technical field [0001] The invention relates to liver cancer drugs, in particular to the application of tamoxifen combined with rapamycin and ginsenoside Rg3 in the preparation of liver cancer drugs. Background technique [0002] Liver cancer is a high-incidence tumor in Jiangsu Province, with rapid progression and high morbidity and mortality. Except for the 5-year survival rate of patients with resectable small liver cancer reaching 80-90%, the average survival time of patients with inoperable liver cancer from the onset of symptoms is only 3-4 months. At present, surgical resection is still the main treatment for liver cancer. However, less than 10% of the total number of liver cancer patients can undergo complete liver cancer resection. The remaining 90% of liver cancer patients cannot be surgically removed, or even if surgical resection of liver cancer is possible, 80% of the patients recurrence within two years of surgery. [0003] Many clinicopathological factors af...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61P35/00A61K31/436A61K31/138
Inventor 郭人花金时代陈小锋朱蔚友束永前王同杉
Owner JIANGSU PROVINCE HOSPITAL
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