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A kind of application of spirulina polysaccharide

A technology of spirulina polysaccharide and spirulina, applied in the application field of spirulina polysaccharide

Active Publication Date: 2014-10-22
YUNNAN PHYTOPHARML +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies on the anti-tumor activity of spirulina polysaccharides have been reported, but most of them are crude polysaccharides. What kind of polysaccharides are the sources of anti-tumor activity, and what is their clear structure, have not been reported

Method used

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  • A kind of application of spirulina polysaccharide
  • A kind of application of spirulina polysaccharide
  • A kind of application of spirulina polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The spirulina polysaccharide of the invention is used to prepare the preparation for inhibiting tumor growth.

[0023] The specific experiment is as follows:

[0024] 1. Anti-S180 mouse sarcoma experiment of crude polysaccharide

[0025] 1. Test materials and methods

[0026] 1.1 Test sample: water-extracted polysaccharide from spirulina, brown dry powder, stored in a desiccator away from light. Spirulina alkali-extracted polysaccharide, light green dry powder, stored in a desiccator away from light.

[0027] The test samples were precisely weighed, and the drug powder was prepared to the highest dose in a sterile workbench, and then diluted to a low dose, and then stored in centrifuge tubes at 4°C, and samples were taken every day for administration.

[0028] 1.2 Experimental animals: 36 ICR mice, 6 weeks old, female, purchased from Shanghai Slack Animal Resource Center.

[0029] 1.3 Dose grouping

[0030] Group compound Animals Route Dose(mg / kg...

Embodiment 2

[0050] Spirulina polysaccharides are used to prepare preparations that have antioxidant effects on cells.

[0051] 1. Polysaccharides on the proliferation of mouse splenocytes

[0052] 1. Test materials and methods

[0053] 1.1 Cell and sample preparation

[0054]Take 6-8 week-old mice, mice with cervical dislocation, quickly take the spleen, put it in a plate of cold HBSS, make it ice-bathed as soon as possible, remove epithelial cells and adipose tissue, grind through a 100-mesh sieve, and centrifuge at 250 g. 5 minutes. Discard the supernatant and wash with hypotonic Gey's (0.15 M NH4Cl, 10 mM KHCO 3 ) solution to eliminate red blood cells, and after about 2 minutes, wash the cells three times with cold HBSS. Add RPMI-1640 culture medium with 10% fetal bovine serum to adjust the concentration of cell suspension to (1 × 10 7 cells / ml), then put 100 μL / well into 96-well culture plate (1 × 10 6 cells / well), then add concanavalin A (C-2010 from Sigma, 5 μg / ml), and set...

Embodiment 3

[0080] Spirulina polysaccharides are used to prepare NF-KB immune preparations. The experiment is as follows:

[0081] Screening of immune activity of spirulina polysaccharide targeting NF-κB signaling pathway

[0082] 1. Materials

[0083] 1.1 Drug configuration: The crude polysaccharide fraction extracted from spirulina water was eluted sequentially with distilled water, 0.2, 0.3, and 0.5 mol / L NaCl solution on a DEAE resin column, and four fractions were obtained for screening for NF-κB immunosuppressive activity. The samples were prepared into 10 mg / ml storage solution with PBS, and stored at -20°C. For experiments, they were diluted with medium to 40 μg / ml, 200 μg / ml, and 1000 μg / ml working concentrations for drug treatment.

[0084] 1.2 Reagents: LPS (Sigma, L6529); CCLR (Reporter Lysis Buffer, Promega, 28303701); Luciferin (Promega, E4550)

[0085] 1.3 Instruments: analytical balance; full-wavelength microplate reader; high-speed centrifuge

[0086] 2. Method

[00...

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Abstract

The invention relates to application of spirulina platensis polysaccharide, which is applied to preparing preparations for restraining tumor growth, preparations with antioxygenation functions to cells and nuclear factor kappa (NF-KB) immune preparations. A spirulina platensis washing gavage administration method has restraining functions on S180 tumors. After administration, spirulina platensis water is used for extracting washing polysaccharide of 100mg / kg, and T / C% is 58.95%. Compared with a control group, the weight of a tumor is obviously different (rho (0.05). Spleen cells of a mice immune organ are used for multiplication experiments of immune cells. Results show that 40, 200 and 1000 ug / ml of spirulina platensis polysaccharide all have functions of promoting multiplication of spleen cells, and when the T / C% is respectively 148.96%, 166.89% and 182.99%, and the T / C% is obviously different from a medicine-free group (rho (0.05). 0.2mol / l of spirulina platensis, 8ug / ml of Nacl elution polysaccharide and 40ug / ml of the Nacl elution polysaccharide have effects on promoting multiplication of spleen cells, and when the T / C% is respectively 19.88% and 202.81%, the T / C% is obviously different from the medicine-free group (rho (0.05).

Description

technical field [0001] The invention relates to an application of spirulina polysaccharide. Background technique [0002] Spirulina is a class of lower plants, belonging to Cyanophyta, Oscillatoriaceae. Like bacteria, they do not have a real nucleus in their cells, so they are also called cyanobacteria. The cell structure of cyanobacteria is primitive and very simple. It grows in the water body, and its morphology can be seen as a spiral filament under the microscope. There have been reports on the antitumor activity of spirulina polysaccharides, but most of them are crude polysaccharides. There is no report on which polysaccharide the antitumor activity is derived from and what its clear structure is. Contents of the invention [0003] The purpose of the present invention is to provide an application of spirulina polysaccharide. [0004] The spirulina polysaccharide of the invention is used to prepare the preparation for inhibiting tumor growth. [0005] The spirulin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P35/00A61P39/06A61P37/04
Inventor 丁侃李俊姚健倪鑫淼
Owner YUNNAN PHYTOPHARML
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