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Herba taraxaci homogeneous polysaccharide and preparation method and application thereof

A dandelion, uniform technology, applied in the field of dandelion homogeneous polysaccharides and its preparation, can solve the problems of anti-complement activity of dandelion and its polysaccharides, achieve broad application prospects and market value, suitable for large-scale production, and safe to use

Inactive Publication Date: 2012-09-05
SHANGHAI UNIV OF T C M
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there is no relevant research report on the anti-complement activity of dandelion and its polysaccharides

Method used

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  • Herba taraxaci homogeneous polysaccharide and preparation method and application thereof
  • Herba taraxaci homogeneous polysaccharide and preparation method and application thereof
  • Herba taraxaci homogeneous polysaccharide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030]Take the whole dandelion herb and place it in the extractor, add ethanol with a volume percentage of 95%, the volume of ethanol added is twice the mass of the whole dandelion herb; carry out reflux defatting for 2 hours, filter, and evaporate the ethanol in the medicinal residue; Add deionized water to the medicinal residues, soak at room temperature for 3 hours, then heat and boil for 4 hours, filter, and repeat the above operation 3 times for the obtained medicinal residues; the volume of water added each time is 10 times the quality of the medicinal residues; combined Extract solution, concentrate the extract solution to a relative density of 1.2 at 80°C, centrifuge, and collect the supernatant; add ethanol with a volume percentage of 95% to the supernatant under constant stirring, and the volume of ethanol added is the supernatant 4 times the volume of the solution; after adding, put it in a refrigerator at 4°C for 12 hours; centrifuge to get the precipitate, and evap...

Embodiment 2

[0091] Take the whole dandelion herb and place it in the extractor, add ethanol with a volume percentage of 95%, and the volume of ethanol added is 3 times the mass of the whole dandelion herb; carry out reflux defatting for 2 hours, filter, and evaporate the ethanol in the medicinal residue; Add deionized water to the medicinal residues, soak at room temperature for 2 hours, then heat and boil for 3 hours, filter, and repeat the above operation 2 times for the obtained medicinal residues; the volume of water added each time is 9 times the quality of the medicinal residues; combine Extract solution, concentrate the extract solution to a relative density of 1.1 at 70°C, centrifuge, and collect the supernatant; add ethanol with a volume percentage of 95% to the supernatant under constant stirring, and the volume of ethanol added is the supernatant 3 times the volume of the solution; after adding, put it in a refrigerator at 4°C for 12 hours; centrifuge to obtain a precipitate, an...

Embodiment 3

[0095] Take the whole dandelion herb and place it in the extractor, add ethanol with a volume percentage of 95%, and the volume of ethanol added is 4 times the mass of the whole dandelion herb; carry out reflux defatting for 2 hours, filter, and evaporate the ethanol in the medicinal residue; Add deionized water to the medicinal residues, soak at room temperature for 1 hour, then heat and boil for 2 hours, filter, and repeat the above operation once for the obtained medicinal residues; the volume of water added is 8 times the mass of the medicinal residues; combine the extracts , concentrate the extract to a relative density of 1.5 at 60°C, centrifuge, and collect the supernatant; add ethanol with a volume percentage of 95% to the supernatant under constant stirring, and the volume of ethanol added is the volume of the supernatant After adding, put it in a refrigerator at 4°C for 12 hours; centrifuge to obtain a precipitate, and evaporate the ethanol; dissolve the precipitate i...

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Abstract

The invention discloses herba taraxaci homogeneous polysaccharide and a preparation method and application thereof. The herba taraxaci homogeneous polysaccharide is prepared by the following steps: performing water extraction and alcohol precipitation and dialysis on herba taraxaci, capturing components of which the molecular weights are more than 10,000 Daltons; separating through anion exchangecolumn chromatography; and purifying through a sephadex chromatographic column. The molecular weight of the homogeneous polysaccharide is 1.6*10<4> Daltons; and monosaccharide mainly comprises arabinose, glucose, galactose, little xylose, rhamnose and mannose. A pharmacodynamics experiment shows that the CP50 value of the classical pathway anticomplement activity is 0.0126 mg / mL, the AP50 value of the alternative pathway anticomplement activity is 0.05885 mg / mL, and the effective removal rate of DPPH free radical is up to 63 percent; and the herba taraxaci homogeneous polysaccharide is expected to be used as an anticomplement active component or / and an antioxidant active component for preparing a health-protetcion food or a medicament preparation and has a wide application prospect and high market value.

Description

technical field [0001] The invention relates to a uniform polysaccharide of dandelion and its preparation method and application, belonging to the technical field of traditional Chinese medicine. Background technique [0002] Dandelion (Herba Taraxaci) is a perennial herb of Compositae, also known as chrysanthemum chrysanthemum and chrysanthemum chrysanthemum. Have heat-clearing and toxic substances removing, reducing swelling and dissipating stagnation, inducing diuresis to relieve stranguria, and are used for boils and swollen toxins, mastitis, scrofula, conjunctival congestion, sore throat, lung abscess, intestinal fistula, damp-heat jaundice, hot stranguria and astringent pain. Modern pharmacological studies have shown that dandelion has the effects of promoting gallbladder and protecting liver, anti-endotoxin, anti-cancer, hypoglycemic, anti-coagulant, diuretic, invigorating spleen and stomach, prebiotics, broad-spectrum antibacterial and immune promotion. [0003] Dan...

Claims

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Application Information

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IPC IPC(8): C08B37/00A23L1/09A61K31/715A61P37/06A61P39/06
Inventor 王顺春刘飞鲍斌王宏伟范洪伟施松善王峥涛胡之璧
Owner SHANGHAI UNIV OF T C M
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