Formula of sika deer tubule frozen sperm diluent containing ginseng polysaccharide and application method of formula
A technology of ginseng polysaccharides and diluents, which can be used in applications, preservation of human or animal bodies, animal husbandry, etc., and can solve problems such as low litter rate, reduced sperm acrosome integrity, and inability to use in large quantities
Inactive Publication Date: 2014-07-16
JILIN AGRICULTURAL UNIV
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Problems solved by technology
However, in the practice of deer production, most of the semen used for artificial insemination of sika deer is frozen semen. Sometimes, although fresh semen is used, due to the long distance and the fact that most deer farmers live in mountainous areas with poor roads, when they arrive at the scene, the semen quality often declines and the sperm If the vigor is lower than the standard (fresh semen vigor is less than 0.6; frozen semen vigor is less than 0.3), insemination will result in low litter rate
Although glycerol has been widely used in the cryopreservation of various cells, glycerol can not only increase the sperm motility of sika deer but also reduce the acrosome integrity of sperm, so it cannot be used in large quantities
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example 1
[0005] Example 1 Preparation of sika deer thin tube frozen semen dilution containing ginseng polysaccharide
[0006] Chemical method Baoding sika deer, the sika deer semen collected by electrical stimulation method, the sika deer semen whose activity is above 0.8 is used for freezing, the dilution factor is determined according to the sperm density, and the semen and isothermal diluent are mixed once before cooling (glycerol The final concentration is guaranteed to be above 3%). Cool and equilibrate in a water bath for 5 hours in a refrigerator at 0°C. Inhale the balanced semen into a 0.25 ml thin tube in the refrigerator, seal it with polyvinyl alcohol powder, place it about 2 cm above the liquid nitrogen surface, freeze it for 5 minutes, and put it into the liquid nitrogen. Sampling was taken and thawed in a water bath at 37°C for 20 seconds, examined under a microscope, and the semen with a viability of 0.35 or higher was marked and stored in a gauze bag.
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The invention relates to a formula of sika deer tubule frozen sperm diluent containing ginseng polysaccharide. The formula of the diluent comprises 20 milligrams of ginseng polysaccharide, 1.94 grams of Tris, 0.8 gram of fructose, 1.08 grams of citric acid, 20 milligrams of yelk, 23 milligrams of GSH (glutathione), 91 milligrams of BSA (bovine serum albumin), 23 milligrams of ATP (adenosine triphosphate), 284 milligrams of vitamin C, 400,000 IU (international unit) of penicillin, 200, 000 IU of streptomycin and 6 milligrams of glycerol, and is added with distilled water so that the total volume of the diluent reaches 100 milligrams. The diluent is favorable for freezing and low-temperature storage of sika deer sperm, plays an important role in increasing reproduction of artificial insemination of sika deer, and has an important significance in developing new applications of ginseng. A preparation method for the sika deer tubule frozen sperm diluent containing the ginseng polysaccharide is simple, production cost is low, and popularization and utilization are facilitated.
Description
technical field [0001] The invention relates to a formula and application method of sika deer thin tube jelly semen diluent containing ginseng polysaccharide in sika deer breeding. Background technique [0002] There are 1 million sika deer in my country, and they are famous all over the world for their high velvet production and good quality. Sika deer is a single-born animal with seasonal estrus, and the antler production varies greatly among individuals. The number of high-yielding deer is small, and it is not uncommon for high-yielding male deer to have poor mating ability. Mating with healthy female deer is infertile or less fertile, which directly affects The quality of deer herds has declined due to the breeding of deer, which makes it more difficult for the propagation of improved breeds. The application of artificial insemination technology to the production of sika deer, and the promotion of artificial insemination and frozen semen of deer for the breeding and imp...
Claims
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IPC IPC(8): A01N1/02
Inventor 郜玉钢张连学赵岩臧埔何忠梅王立岩李萍张爱华雷锋杰杨鹤蔡恩博李学
Owner JILIN AGRICULTURAL UNIV