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Malachite green detecting method and application

A malachite green and detection method technology, which is applied in the preparation of test samples, Raman scattering, material excitation analysis, etc., can solve the problems of high cost, time-consuming and labor-consuming detection methods, etc., so as to save detection cost and shorten detection time. Effects of time and high detection sensitivity

Inactive Publication Date: 2012-09-12
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the characteristics of time-consuming, labor-intensive and expensive cost of current detection methods, the research and development of a new detection method for malachite green that is easy to operate, fast and accurate is of great significance for strengthening the monitoring of aquatic product quality and safety and promoting the development of aquatic product trade.

Method used

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  • Malachite green detecting method and application
  • Malachite green detecting method and application
  • Malachite green detecting method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Accurately weigh 10.00mg into a 100mL beaker, dissolve it completely with a certain volume of acetonitrile (chromatographically pure), and finally set the volume in a 100mL volumetric flask to prepare a 100mg / Kg malachite green mother liquor, and then use a certain volume ratio of acetonitrile and super Pure water is used as a solvent to dilute the mother liquor to 0.5, 1, 5, 20ng·g -1 , use a pipette gun to draw 0.1-0.5μL standard solution, drop it on the substrate with SERS activity modified by gold nanoparticles, and after the solution evaporates naturally, put it into a Raman spectrometer for detection. Depend on figure 1 It can be seen that the intensity of surface-enhanced Raman spectra increases with the increase of concentration. With its 1610, 1360, 1170, 798, 913, 432cm -1 Based on the fact that the characteristic peak is still visible, the method can detect at least 0.5-1ng·mL -1 malachite green.

Embodiment 2

[0021] A series of concentrations (0, 0.5, 1, 2, 5, 10, 20 ng g -1 ) of recessive malachite green. The simplified international standard extraction method is as follows: cut the aquatic product sample, use acetonitrile and ammonium acetate buffer solution for vibration extraction, then centrifuge, repeat twice, combine the upper layer extract; extract the extract with dichloromethane, repeat twice, Combine the organic phases of the lower layer and evaporate to dryness, the extract is oily; add acetonitrile to re-dissolve the oily extract; then add a certain concentration of dichlorodicyanobenzoquinone to oxidize recessive malachite green into malachite green, and wait until it is completely oxidized Obtain the solution to be tested; wherein in the acetonitrile and ammonium acetate buffer solution, the volume ratio of the total volume of acetonitrile and ammonium acetate is about 10:1; the total volume of dichloromethane in the liquid-liquid extraction and the volume ratio of t...

Embodiment 3

[0024] A series of concentrations (0, 0.5, 1, 2, 5, 10, 20 ng g -1 ) of recessive malachite green. The simplified international standard extraction method is as follows: cut the aquatic product sample, use acetonitrile and ammonium acetate buffer solution for vibration extraction, then centrifuge, repeat twice, combine the upper layer extract; extract the extract with dichloromethane, repeat twice, Combine the organic phases of the lower layer and evaporate to dryness, the extract is oily; add acetonitrile to re-dissolve the oily extract; then add a certain concentration of dichlorodicyanobenzoquinone to oxidize recessive malachite green into malachite green, and wait until it is completely oxidized Obtain the solution to be tested; wherein in the acetonitrile and ammonium acetate buffer solution, the volume ratio of the total volume of acetonitrile and ammonium acetate is about 10:1; the volume ratio of the total volume of dichloromethane in the liquid-liquid extraction and t...

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Abstract

The invention discloses a malachite green detecting method, which is characterized by comprising the following steps of: dripping a standard malachite green solution on a Raman substrate with surface activity, which is modified by nanometer gold particles; and after a solvent is evaporated to be dry, collecting a surface-enhanced Raman spectrum of malachite green by using a con-focal microscope Raman spectrometer with a laser wavelength of 633 nm, wherein the surface-enhanced Raman spectrum of the malachite green is provided with characteristic peaks at positions of 1610, 1360, 1170, 798, 913 and 432 cm<1>. The invention further provides an application of the detecting method, which is used for detecting a malachite green or hidden malachite green residual amount in a water body. By combining an SERS (Surface-Enhanced Raman Scattering) active substrate modified by the nanometer gold particles with the surface-enhanced Raman spectrum technology, the detecting method provided by the invention is applied to detecting residual malachite green and hidden malachite green of a fishery medicine of an aquatic product. The detecting method has higher detection sensitivity, so that the detection time is greatly reduced, and the detection cost is saved.

Description

technical field [0001] The invention relates to the technical field of malachite green detection, in particular to a method for detecting malachite green by surface-enhanced Raman spectroscopy and an application of the method. Background technique [0002] China is the world's largest producer and exporter of aquatic products, but in recent years, frequent outbreaks of aquatic product safety incidents not only endanger the interests of consumers, but also become a bottleneck restricting the normal export of aquatic products in my country. Among the many safety incidents of aquatic products, the excessive use of fishery medicines due to inappropriate use, excessive use or even illegal use of fishery medicines is the most serious phenomenon. Malachite green, because of its carcinogenic, teratogenic, and sudden-induced hazards to the human body, has been banned from being used in aquaculture by many countries including China, but because of its low price and good efficacy, It ...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N1/28
Inventor 黄轶群赖克强张源园张衍亮
Owner SHANGHAI OCEAN UNIV
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