Malachite green detecting method and application
A malachite green and detection method technology, which is applied in the preparation of test samples, Raman scattering, material excitation analysis, etc., can solve the problems of high cost, time-consuming and labor-consuming detection methods, etc., so as to save detection cost and shorten detection time. Effects of time and high detection sensitivity
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[0018] Example 1
[0019] Accurately weigh 10.00mg in a 100mL beaker, dissolve it completely with a certain volume of acetonitrile (chromatographically pure), and finally set the volume in a 100mL volumetric flask to prepare a 100mg / Kg malachite green mother liquor, and then use a certain volume ratio of acetonitrile and ultra Pure water is used as solvent to dilute the mother liquor to 0.5, 1, 5, 20ng·g -1 , suck 0.1-0.5 μL of the standard solution with a pipette, drop it on the substrate with SERS activity modified by nano-gold particles, and put it into a Raman spectrometer for detection after the solution is naturally evaporated and dried. Depend on figure 1 It can be seen that the surface-enhanced Raman spectral intensity increases with increasing concentration. With its 1610, 1360, 1170, 798, 913, 432cm -1 Based on the characteristic peaks still visible, this method can detect at least 0.5-1 ng·mL -1 malachite green.
Example Embodiment
[0020] Example 2
[0021] A series of concentrations (0, 0.5, 1, 2, 5, 10, 20 ng g -1 ) in recessive malachite green. The simplified international standard extraction method is as follows: cut aquatic product samples, use acetonitrile and ammonium acetate buffer solution for shaking extraction, then centrifuge, repeat twice, and combine the upper extract; extract the extract with dichloromethane, repeat twice, Combine the lower organic phases by rotary evaporation to dryness, and the extract is oily; add acetonitrile to re-dissolve the oily extract; then add a certain concentration of dichlorodicyanobenzoquinone to oxidize the hidden malachite green to malachite green, after the oxidation is complete The solution to be tested is obtained; in the acetonitrile and ammonium acetate buffer solution, the volume ratio of the total volume of acetonitrile to ammonium acetate is about 10:1; the volume ratio of the total volume of dichloromethane to the extraction solution in liquid-li...
Example Embodiment
[0023] Example 3
[0024] A series of concentrations (0, 0.5, 1, 2, 5, 10, 20 ng g -1 ) in recessive malachite green. The simplified international standard extraction method is as follows: cut aquatic product samples, use acetonitrile and ammonium acetate buffer solution for shaking extraction, then centrifuge, repeat twice, and combine the upper extract; extract the extract with dichloromethane, repeat twice, Combine the lower organic phases by rotary evaporation to dryness, and the extract is oily; add acetonitrile to re-dissolve the oily extract; then add a certain concentration of dichlorodicyanobenzoquinone to oxidize the hidden malachite green to malachite green, after the oxidation is complete The solution to be tested is obtained; in the acetonitrile and ammonium acetate buffer solution, the volume ratio of the total volume of acetonitrile to ammonium acetate is about 10:1; the volume ratio of the total volume of dichloromethane to the extraction solution in liquid-li...
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