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High-sugar and high-acid resistant lactobacillus

A Lactobacillus, high sugar and high acid technology, applied in the field of microorganisms, can solve the problems of low optical purity, low L-lactic acid conversion rate, etc., achieve high fermentation efficiency, improve sugar conversion rate and lactic acid production rate, and good physiological and biochemical functions Effect

Active Publication Date: 2012-09-19
刘锋 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this invention, the conversion rate of L-lactic acid is on the low side, and the optical purity is on the low side

Method used

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  • High-sugar and high-acid resistant lactobacillus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 The preparation method of lactobacillus provided by the present invention.

[0022] one. Materials and Methods

[0023] 1. Strain: Lactobacillus delbrueckii (laboratory preservation strain, self-named F-A10)

[0024] 2. Medium formulation and culture conditions

[0025] Separation and detection medium--GYP medium: glucose 1.0g, yeast extract 1.0g, peptone 0.5g, meat juice extract 0.2g, NaAc 3H 2 O 0.2g, MgSO 4 7H 2 O 20mg, MnSO 4 4H 2 O 1mg, FeSO 4 7H 2 O 1mg, NaCl 1mg, Tween 80 analytical grade 0.5ml, agar 1.2g, CaCO 30 0.5g, water 100ml; culture conditions: temperature 121°C, culture time 20min.

[0026] Seed medium formula (unit: g / l): glucose 50, yeast extract 10, peptone 10, gravy extract 0.2, NaAc 3H 2 O 0.5, MgSO 4 7H 2 O 0.2, MnSO 4 4H 2 O 0.01, FeSO 4 7H 2 O 0.01 NaCl 0.01; culture conditions: PH=4.0-5.0, temperature 121°C, culture time 20min.

[0027] Fermentation medium formula (unit: g / l): glucose 200, ammonium sulfate 1.44, d...

Embodiment 2

[0073] The screened strains were connected to 150m 3 Fermentation in a fermenter, the fermentation temperature is 54°C, sodium hydroxide is used as the neutralizing agent, the stirring speed is 55-65r / min, and the pH is 4.0-5.0.

[0074] The culture medium adopts fermentation medium, formula (unit: g / l): glucose 200, ammonium sulfate 1.44, dipotassium hydrogen phosphate 0.08, potassium dihydrogen phosphate 0.08, yeast extract 0.2; culture conditions: PH=4.0-5.0, temperature 121 ℃, the incubation time was 20 min. The results are shown in Table 8.

[0075] group item Period (h) Acid production (g / l) Residual sugar rate (%) Volume (m3) Conversion rate(%) Optical purity (%) Group 7 23.5 192.5 0.09 124.5 97.29 99.38 Group 8 22.5 190.5 0.11 128 97.30 99.42 Group 9 23 189.8 0.10 127.5 95.51 99.39

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Abstract

The invention provides a high-sugar and high-acid resistant lactobacillus. The high-sugar and high-acid-resistant lactobacillus can still keep the high fermentation efficacy under the high-sugar and high-acid environment so as to improve a sugar conversion rate and a lactic acid generation rate, the optical purity of the L-lactic acid is improved, a fermentation period is shortened, and time and cost are saved. When the high-sugar and high-acid resistant lactobacillus is used for industrial mass production, the lactobacillus disclosed by the invention can still keep good physiological and biochemical functions and high fermentation efficiency and is suitable for the industrial production.

Description

technical field [0001] The invention relates to a novel lactobacillus and its application in a fermentation process, belonging to the field of microorganisms. Background technique [0002] Lactic acid is the smallest chiral molecule in nature. Its structure contains an asymmetric carbon atom, so it has optical activity. According to its configuration, it can be divided into D-lactic acid and L-lactic acid. L-lactic acid is an intermediate product of mammalian metabolism. Since the human body can only metabolize L-lactic acid, it is necessary to replace D, L-lactic acid with L-lactic acid when used in the food and pharmaceutical industries. The most potential application of L-lactic acid is the production of polylactic acid (PLA), the manufacture of biodegradable plastics. In recent years, people have gained a deep understanding of the physical and chemical properties and physiological activities of D-lactic acid, L-lactic acid and their derivatives, and broadened the enanti...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/01C12N13/00C12P7/56C12R1/225
Inventor 刘锋李进刘海涛程小龙张亚巍
Owner 刘锋
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