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Method for enzymatic synthesis of feruloyl oligosaccharide in mixed solvent

A technology of ferulic acid sugar ester and mixed solvent, which is applied in the field of enzymatic synthesis of ferulic acid sugar ester, can solve the problems of harsh conditions, poor control, cumbersome steps, etc., achieve mild reaction conditions, easy separation, and avoid reaction conditional effect

Inactive Publication Date: 2012-09-19
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical preparation method of glucosyl ferulic acid involves a selective combination of glucose hydroxyl groups, which is not easy to control, and requires the protection and deprotection of hydroxyl groups. The steps are cumbersome and the conditions are harsh.

Method used

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  • Method for enzymatic synthesis of feruloyl oligosaccharide in mixed solvent
  • Method for enzymatic synthesis of feruloyl oligosaccharide in mixed solvent
  • Method for enzymatic synthesis of feruloyl oligosaccharide in mixed solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The synthesis of ferulic acid glucose ester catalyzed by different enzymes, the specific steps are as follows:

[0033] (1) Will After the molecule is activated and dried, it is added to the reaction solvent to be used, and the solvent is dried to obtain a dehydrated organic solvent after four days. The amount of molecular sieve used is 200 mg / ml.

[0034] (2) Enzymatic transesterification reaction: Take 3.8g of glucose and 1g of vinyl ferulic acid and add them to 100ml of dehydrated organic solvents of equal volume pyridine and tert-butanol, shake and dissolve, then mix, then add 2g of various enzymes, Alkaline protease produced by Bacillus subtilis (200,000, Wuxi Xuemei Technology Co., Ltd.); Lipase from Candida Cylindacea (5.18u / mg, sigma-Aldrich); Lipase from Candida rugosa (1104u / mg, sigma); Lipase from Rhizopus oryzae (55.7u / mg, sigma-Aldrich); Lipase Novozym 435 (7u / mg, Guangzhou Mingyuan Industry and Trade Co., Ltd.); Lipase from Pseudomonas fluorescens (2.2u / ...

Embodiment 2

[0036] Different organic solvents catalyze the enzymatic synthesis of ferulic acid sugar ester, and the specific steps are as follows:

[0037] (1) Will After molecular sieve activation 4 is dried, it is added to the reaction solvent to be used, and the solvent is dried to obtain a dehydrated organic solvent after four days. The amount of molecular sieve used is 200 mg / ml.

[0038] (2) Enzymatic transesterification reaction: Add 3.8g~58g of glucose and 1g of ferulic acid to 100ml of different dehydrated organic solvents pyridine, tert-butanol, and 100ml of equal volumes of mixed solvents of tert-butanol and pyridine, and then add 2g of lipase Novozyon 435 (7u / mg); add 20g of molecular sieves, shake at 220r / min for 72h at 50°C. After the reaction is finished, the molecular sieve and enzyme are removed by filtering with filter paper, and the obtained filtrate is evaporated by a rotary evaporator to remove the organic solvent, and then purified by a silica gel column to obtain ...

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Abstract

The invention relates to a method for enzymatic synthesis of feruloyl oligosaccharide in a mixed solvent. The method includes: (1) adding an activated molecular sieve in an organic solvent, and performing dehydration processing for 24-96 hours to obtain the dehydrated organic solvent; (2) performing enzymatic transesterification reaction, adding ferulic acid vinyl ester and glucose in the dehydrated organic solvent according to molar ratio of 2:1-1:6, then adding enzyme and the activated molecular sieve, performing oscillatory reaction for 10-144 hours at the temperature of 30-70 DEG C, filtering after the reaction is finished, taking clear filter liquid to perform rotary evaporation so as to remove the organic solvent, and purifying through a silicagel column. The method is simple to operate and environment-friendly, catalyst enzyme can be reused, and a structure of an obtained product is good in unicity.

Description

technical field [0001] The invention belongs to the field of preparation of sugar esters of ferulic acid, in particular to a method for enzymatically synthesizing sugar esters of ferulic acid in a mixed solvent. Background technique [0002] Sugar ester of ferulic acid has good antioxidant effect, it can chelate metal ions, scavenge free radical ions DPPH, and OH and H 2 o 2 , and its antioxidant properties are dose-dependent with the dosage of sugar esters of ferulic acid, and have higher antioxidant properties than free ferulic acid. Sugar esters of ferulic acid have amphiphilic properties, which expands its application range from water-soluble to fat-soluble, making it applicable to anti-oxidative cosmetics. [0003] Sugar esters of ferulic acid are initially extracted from finished fibers such as wheat bran by enzymatic method, acid method, and cooking method. Most of the extracted sugar esters of ferulic acid are ferulic acid oligosaccharides. As for the chemical pre...

Claims

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Application Information

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IPC IPC(8): C12P19/02
Inventor 朱利民石萌权静
Owner DONGHUA UNIV
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