Antihypertensive rapeseed peptide and preparation method and application of antihypertensive rapeseed peptide

A technology for lowering blood pressure and rapeseed, which is applied in cardiovascular system diseases, hydrolyzed protein components, fermentation, etc., can solve the problem of less blood pressure lowering drugs, and achieve the effects of easy digestion and absorption, environmental protection, and a wide range of raw material sources.

Active Publication Date: 2014-03-19
NANJING UNIV OF FINANCE & ECONOMICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are fewer blood pressure-lowering drugs based on renin inhibitors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 Using proteinase K to prepare hypotensive rapeseed peptide

[0025] (1) Pulverize the rapeseed meal and pass it through a 100-mesh sieve to make the crushed rapeseed meal. Using crushed rapeseed meal as raw material, mix the raw material with water at a solid-to-liquid ratio of 1:20, adjust the pH value to 7.5, and stir and disperse for 1 hour at a temperature of 37°C to obtain a rapeseed protein aqueous solution. The raw material of the rapeseed meal is common rapeseed or double-low rapeseed, and the particle size of the crushed rapeseed meal is 10-200 μm. The raw material of the aqueous solution of rapeseed protein may also be protein separated from rapeseed meal.

[0026] (2) According to the mass ratio of protease to protein in the rapeseed protein aqueous solution of 5:100, add proteinase K to the rapeseed protein aqueous solution described in step (1), control the temperature and pH value, and hydrolyze for 1 to 3 h . After the reaction, the hydrolyza...

Embodiment 2

[0034] Example 2 Preparation of Rapeseed Peptide for Lowering Blood Pressure by Thermolysin

[0035] (1) Pulverize the rapeseed meal and pass it through a 100-mesh sieve to make the crushed rapeseed meal. Using crushed rapeseed meal as raw material, mix the raw material with water at a solid-to-liquid ratio of 1:20, adjust the pH value to 9.0, and stir and disperse for 1 hour at a temperature of 50°C to obtain a rapeseed protein aqueous solution. The raw material of the rapeseed meal is common rapeseed or double-low rapeseed, and the particle size of the crushed rapeseed meal is 10-200 μm. The raw material of the aqueous solution of rapeseed protein may also be protein separated from rapeseed meal.

[0036] (2) According to the mass ratio of protease to protein in the rapeseed protein aqueous solution of 1:100, add thermolysin to the rapeseed protein aqueous solution described in step (1), control the temperature and pH value, and hydrolyze for 4 h. After the reaction, the h...

Embodiment 3

[0038] Example 3 Preparation of blood pressure-lowering rapeseed peptide crude extract by composite protease

[0039] (1) Pulverize the rapeseed meal and pass it through a 100-mesh sieve to make the crushed rapeseed meal. Using crushed rapeseed meal as raw material, mix the raw material with water at a solid-to-liquid ratio of 1:20, adjust the pH value to 9.0, and stir and disperse for 1 hour at a temperature of 50°C to obtain a rapeseed protein aqueous solution. The raw material of the rapeseed meal is common rapeseed or double-low rapeseed, and the particle size of the crushed rapeseed meal is 10-200 μm. The raw material of the aqueous solution of rapeseed protein may also be protein separated from rapeseed meal.

[0040] (2) According to the mass ratio of protease to protein in the rapeseed protein aqueous solution of 4:100, add thermolysin to the rapeseed protein aqueous solution described in step (1), control the temperature and pH value unchanged, and hydrolyze for 4 h ...

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Abstract

The invention provides an antihypertensive rapeseed peptide and a preparation method and an application of the antihypertensive rapeseed peptide. The method for preparing the antihypertensive rapeseed peptide includes using protease to hydrolyze a rapeseed protein water solution, then performing refining to obtain the antihypertensive rapeseed peptide. When the method for preparing the antihypertensive rapeseed peptide is compared with methods for preparing the antihypertensive rapeseed peptide in prior art, rapeseed meals or rapeseed isolated proteins are directly used as raw materials to obtain the antihypertensive rapeseed peptide by means of enzymatic hydrolysis, the sources of raw materials are wide, the production process is simple, the cost is low, the waste can be changed into the valuable, and environment protection is facilitated. The antihypertensive rapeseed peptide can be used for not only inhibiting angiotensin converting enzyme (ACE) activity, but also inhibiting renin activity to block an alternative pathway of a renin angiotensin system (RAS), the alternative pathway of the RAS is activated due to the fact that hypotensor of ACE inhibitor is taken for a long time, and the antihypertensive rapeseed peptide has a great developing prospect in decreasing blood pressure.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a blood pressure-lowering rapeseed peptide and a preparation method and application thereof. Background technique [0002] my country is the country with the largest rapeseed production in the world, with an annual output of nearly 12 million tons. Rapeseed meal is the main by-product of rapeseed oil processing, accounting for 65% of the total rapeseed, which contains 35% to 42% protein. The amino acid composition of rapeseed protein is reasonable, and the content of lysine, cystine and methionine is relatively high, so it is an excellent vegetable protein. The development and utilization of rapeseed protein resources has great social significance and good economic benefits for the effective use of our country's plant protein resources and the improvement of the nutritional level of our people. [0003] At present, there are about 200 million hypertensive patients in my country, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06A61K38/01A61P9/12
Inventor 鞠兴荣何荣王立峰袁建高瑀珑陈银基都立辉石嘉怿周建新王雪峰
Owner NANJING UNIV OF FINANCE & ECONOMICS
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