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Production method of triple inactivated vaccine for newcastle disease, infectious bursal disease and viral arthritis

A technology for bursal disease and chicken Newcastle disease, applied in antiviral agents, viral antigen components, pharmaceutical formulations, etc., can solve the problems of uncontrollable epidemic development, high mortality rate, slow growth, etc., to reduce the occurrence of epidemics and The effect of spreading, rapid antibody production, and long protection period

Inactive Publication Date: 2012-09-26
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In chickens with acute disease, especially breeder chickens, often due to slow growth or stagnation, weight loss, low feeding utilization rate and high death rate, etc., it brings huge economic losses to the chicken industry.
[0005] There are many varieties of commercialized products on the market at home and abroad, but they are all single traditional vaccines. Multiple use of vaccines, especially inactivated vaccines, not only increases the burden of manpower and material resources on the chicken farm, but also causes the injection stress of catching chickens many times. Can affect performance and increase susceptibility of flocks to disease
Coupled with the continuous mutation of virus strains in recent years, although a variety of vaccines have been introduced, the development of the epidemic is still uncontrollable.

Method used

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  • Production method of triple inactivated vaccine for newcastle disease, infectious bursal disease and viral arthritis
  • Production method of triple inactivated vaccine for newcastle disease, infectious bursal disease and viral arthritis
  • Production method of triple inactivated vaccine for newcastle disease, infectious bursal disease and viral arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] (1) Antigen preparation and inspection of semi-finished products:

[0087] 1. Preparation of Chicken Newcastle Disease Virus Liquid

[0088] (1) Inoculate the virus seeds used for production, and use sterilized saline to make 10 -4 Dilute and inoculate 200 10-day-old susceptible chicken embryos, 0.1ml per embryo, seal the pinholes after inoculation, and continue to incubate at 37°C without turning the embryos.

[0089] (2) Incubation and observation After the chicken embryos were inoculated, the embryos were photographed once a day, and 11 chicken embryos that died 60 hours ago were discarded. After that, the embryos were photographed once every 4-6 hours, and the dead embryos were taken out at any time until 96 hours, a total of 155 dead embryos were taken out, and all of them were taken out, with the air chamber upward, and cooled at 2-8°C for 12 hours.

[0090] (3) Harvesting Take out the cooled chicken embryos and harvest the allantoic fluid of the chicken embryo...

Embodiment 2

[0123] Finished product inspection - the laboratory trial-produced a batch of triple inactivated vaccine about 6000ml, the batch number is 2010001.

[0124] 1. character

[0125] Appearance Milky white emulsion.

[0126] Dosage form water-in-oil type. Take a clean straw, suck a small amount of vaccine and drop it into cold water, except for the first drop, it will not spread.

[0127] Stability Take 10ml of the vaccine and add it to a centrifuge tube, centrifuge at 3000r / min for 15 minutes, and the water phase precipitated at the bottom of the tube is 0.1ml.

[0128] The viscosity is carried out according to the appendix of the current "Chinese Veterinary Pharmacopoeia", and the viscosity is 52cP, which meets the regulations.

[0129] 2. Filling volume inspection According to the appendix of the current "Chinese Veterinary Drug Code", the results of the measurement of the three bottles were 251.3ml, 252.1ml, and 251.5ml, which met the regulations.

[0130] 3. Sterility te...

Embodiment 3

[0147] We have carried out a comparative test of the immune effect of the triple vaccine and each component single vaccine (commercialized product produced by the inventor's company), the test method is as follows:

[0148] 50 21-day-old SPF chickens were used, and 10 chickens were immunized with triple vaccine, Newcastle disease, infectious bursal disease, and viral arthritis single vaccine, and 0.5ml of vaccine was injected subcutaneously per chicken, and the other 10 chickens were not immunized as a control. Breeding in groups. Chickens in each group were blood collected before immunization and 7, 14, 21, 28, 35 days after immunization, serum was separated, and serum antibodies were measured. The results are compared in Table 2 below.

[0149] Table 2 The results of the comparative test of the immune effect of the triple vaccine and the single vaccine of each component

[0150]

[0151] Note:

[0152] Bacterial endotoxin test by gel method

[0153] Pyrosate Endotoxin...

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Abstract

The invention relates to a production method of a triple inactivated vaccine for newcastle disease, infectious bursal disease and viral arthritis. In the invention, the method adopts newcastle disease virus La Sota strain, escherichia coli genetically engineered bacteria E.coli BL21 / pET28a-VP2 strain expressing chicken infectious bursal disease virus VP2 protein as well as viral arthritis AV2311 strain as the bacteria producing virus seeds; and the inactivated vaccine is prepared by use of super concentration technology and high-quality adjuvant. After animal immunization, the antibody is quickly produced; and the inactivated vaccine has high titer and long protection term, and reduces the occurrence and propagation of pestilence.

Description

technical field [0001] The invention relates to a preparation method of an oil emulsion vaccine used for preventing Newcastle disease, infectious bursal disease and viral arthritis in chickens, and belongs to the field of veterinary biological products. Background technique [0002] Newcastle disease (ND) has a high morbidity and fatality rate. It occurs frequently all over the world. Once it breaks out, it will bring a devastating blow to the poultry industry. It is a major infectious disease that endangers the poultry industry. OIE lists it as a Category A disease. [0003] Infectious bursal disease virus (IBD) is a special disease caused by infectious bursal disease virus in the family BiRNAviridae, which damages the bursa of young chickens. It is characterized by diarrhea, exhaustion, significant inflammation and enlargement of the bursa of Fabricius first, followed by atrophy, and finally the death of affected chickens. In addition to causing the death of some chicks,...

Claims

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Application Information

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IPC IPC(8): A61K39/295A61P31/14A61K39/12A61K39/15A61K39/17
Inventor 左青山李晓林王红范根成宫晓郭伟伟李芳
Owner YEBIO BIOENG OF QINGDAO
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