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Preparation method of yeast beta-1,3-D-glucan derivative

A technology of dextran and derivatives, applied in the field of biochemical preparation, can solve the problems of endangering workers' health, complicated process, and corrosion of equipment, and achieve the effects of increasing extraction rate, shortening extraction time, and reducing extraction cost

Inactive Publication Date: 2012-09-26
郭宏昌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Someone used Sevage method, acid method and alkali method to compare and test respectively, and found that it is difficult to extract β-1,3-D-glucan from brewer's yeast by Sevage method; the yield of crude polysaccharide obtained by acid method extraction is high , but contains a large amount of mannan and protein, the purity is not high, the process is too complicated, and the acid method corrodes the equipment, endangering the health of workers; the alkaline extraction process is simple, and the product is relatively high-purity β-1,3-D-glucose Glycans, an ideal pathway for efficient β-1,3-D-glucan from spent yeast

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Alkali treatment: take 100g of yeast and add 200-300ml of 1M sodium hydroxide, act at a temperature of 80-100°C for 2h, centrifuge at a speed of 3000r / min for 15min, wash with water twice, filter and centrifuge, and collect the precipitate;

[0027] 2. Microwave puffing: Put the alkali-treated yeast collected from the sediment into a microwave oven for puffing. The microwave processing power is high, and the microwave processing time is 8 minutes; pay attention to controlling the microwave processing power and time. During puffing, use a special microwave spoon to stir Yeast, so that it is evenly heated;

[0028] 3. Neutralization treatment: Cool down the puff obtained in step 2. When the temperature is lower than 50°C, slowly add acetic acid with a volume fraction of 20% for neutralization. The amount of acetic acid added is to reduce the pH value of the extract to 7.0 Time;

[0029] 4. Separation: Centrifuge the neutralizing solution at a speed of 4 000 r / min, col...

Embodiment 2

[0033] 1. Alkali treatment: 100g yeast, add 1M sodium hydroxide 200-300ml, temperature 80-100℃, centrifuge at 3000r / min for 15min after 2h, wash with water twice, filter and centrifuge, and collect the precipitate;

[0034] 2. Expansion by twin-screw extruder: the yeast collected by sedimentation is slowly added to the extruder for expansion, the screw speed is 30HZ, and the expansion temperature is 120-130°C. The puffing machine is a twin-screw puffing machine, the screw speed is 30HZ, and the puffing temperature is controllable;

[0035]3. Neutralization treatment: The above-mentioned extracts of 1 and 2 are subjected to cooling treatment. When the temperature is lower than 50°C, slowly add acetic acid with a volume fraction of 20% for neutralization. The amount of acetic acid added is to reduce the pH value of the extract. until 7.0;

[0036] 4. Separation: Centrifuge the neutralizing solution at a speed of 4 000 r / min, collect and combine the centrifuged solution, heat an...

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Abstract

The invention discloses a preparation method of a yeast beta-1,3-D-glucan derivative. Puffing technology and acid-base solution treatment are combined in the method to prepare the yeast beta-1,3-D-glucan derivative, so that the time for preparing the yeast beta-1,3-D-glucan can be greatly saved, and the extraction cost can be reduced.

Description

technical field [0001] The invention belongs to the field of biochemical preparation. It relates to a process for preparing yeast β-1,3-D-glucan organisms by combining extruding technology with acid-base solution treatment. Background technique [0002] Yeast is an important microorganism in the food industry. Its cell wall is divided into three layers from the outside to the inside, which are mannan, protein and glucan. There are three types of dextran in the innermost layer: alkali-soluble dextran, alkali-insoluble, acid-soluble dextran, and acid-base insoluble dextran. Among them, the acid-base insoluble dextran is the active polysaccharide, except for a small amount of β- Except for 1, 6 bonds, almost all of them are β-1, 3 bonds, so it is also called β-(1, 3)-glucan. Yeast β-1, 3-D-glucan can enhance the immunity of mammals, anti-cancer, anti-bacteria, anti-virus, anti-fungal, anti-parasite, reduce cholesterol and blood lipids, promote wound healing, etc., is a good ...

Claims

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Application Information

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IPC IPC(8): C08B37/02A61P37/02A61P35/00A61P31/12A61P39/00
Inventor 郭宏昌
Owner 郭宏昌
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