The invention discloses a 1,3-beta-
D glucan derivative, a kit, a preparation method and a method for determining the content of 1,3-beta-
D glucan. The invention provides a 1,3-beta-
D glucan magnetic particle chemiluminiscence
immunoassay kit which combines a magnetic
particle separation technology, a
biotin-
avidin amplification technology and a chemiluminiscence analysis technology. Meanwhile, a biotinylated 1,3-beta-D
glucan antigen derivative and an
enzyme-labeled 1,3-beta-D
glucan antigen derivative are prepared from the 1,3-beta-D
glucan antigen derivative, so that two systems (1, the biotinylated 1,3-beta-D glucan antigen derivative plus an
antibody enzyme-labeled
reagent and plus a calibration solution; 2, the
enzyme-labeled 1,3-beta-D glucan antigen derivative plus a biotinylated 1,3-beta-D glucan
mouse monoclonal antibody and plus calibration liquid) are constructed, and the 1,3-beta-D glucan in a sample can be tested, so that different enterprises have more flexible selection when developing the kit, and the kit can be realized by only preparing the intermediate, namely, the 1,3-beta-D glucan antigen derivative.