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Preparation method of anticlotting materials

An anticoagulant and anticoagulant technology, applied in the field of biomedical engineering materials, to achieve the effects of easy realization, excellent endothelial cell adhesion and proliferation, and definite anticoagulation effect

Inactive Publication Date: 2012-10-03
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few reports on the surface modification of anticoagulant materials with bivalirudin. In order to broaden the application window of anticoagulant devices, the present invention discloses a thrombin inhibitor bivalirudin for surface modification of anticoagulant materials. Methods

Method used

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  • Preparation method of anticlotting materials
  • Preparation method of anticlotting materials
  • Preparation method of anticlotting materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 A preparation method of an anticoagulant material. The steps are:

[0017] Dissolve bivalirudin at 0.1 μg / ml in WSC solution, the composition of WSC solution is 9.76 mg / ml of 2-(N-morpholine) ethanesulfonic acid (MES) buffer solution, 1 mg / ml of 1-ethane 3-(3-dimethylaminopropyl) carbodiimide (EDC) and 0.24 mg / ml of N-hydroxysuccinamide (NHS). The 316L stainless steel with plasma polyallylamine coating was soaked in bivalirudin-WSC solution for 1 hour to react, then fully rinsed with PBS and distilled water with a pH value of 7.4, and dried.

Embodiment 2

[0018] Example 2 A preparation method of an anticoagulant material. The steps are:

[0019] Dissolve bivalirudin at 10 mg / ml in WSC solution, the composition of WSC solution is 9.76 mg / ml of 2-(N-morpholine) ethanesulfonic acid (MES) buffer solution, 1 mg / ml of 1-ethyl - 3-(3-dimethylaminopropyl) carbodiimide (EDC) and 0.24 mg / ml of N-hydroxysuccinamide (NHS). The 316L stainless steel coated with plasma polyallylamine was immersed in the bivalirudin-WSC solution for 48 hours to react, and then fully rinsed with PBS and distilled water with a pH value of 7.4, and dried to obtain the final product.

Embodiment 3

[0020] Embodiment 3 A preparation method of an anticoagulant material. The steps are:

[0021] Dissolve bivalirudin at 400 μg / ml in WSC solution, the composition of WSC solution is 9.76 mg / ml of 2-(N-morpholine) ethanesulfonic acid (MES) buffer solution, 1 mg / ml of 1-ethyl - 3-(3-dimethylaminopropyl) carbodiimide (EDC) and 0.24 mg / ml of N-hydroxysuccinamide (NHS). The 316L stainless steel coated with plasma polyallylamine was immersed in the bivalirudin-WSC solution for 24 hours to react, and then fully rinsed with PBS and distilled water with a pH value of 7.4, and dried to obtain the obtained product.

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Abstract

The invention discloses a preparation method of anticlotting materials, which is used for performing the anticlotting modification on the surface of an implanting device material so as to improve the anticoagulant activity of an intravascular stent. The preparation method comprises the following steps: dissolving 0.1mug / ml-10mg / ml of bivalirudin in a water soluble carbohydrate (WSC) solution, wherein the WSC solution is composed of 9.76mg / ml of 2-(N-morpholine) ethanesulfonic acid buffer solution, 1mg / ml of 1-ethyl-3-(3-dimethylamino propyl) carbodiimide and 0.24mg / ml of N-hydroxy-succinamide; obtaining bivalirudin-WSC solution; immersing the biomedical materials containing amido on surface in the bivalirudin-WSC solution to react for 1-48 hours, and then fully rinsing the biomedical materials by phosphate buffer solution (PBS) with pH value of 7.4 and distilled water respectively, and drying to obtain the objective matter. The anticlotting materials or an apparatus obtained by the invention can play the role of anticlotting by directly or specially imhibiting the activity of thrombin. The preparation method of the anticlotting materials is definite in the anticlotting effect, safe in use and simple in operation of the method, and free from the limit of the physical structure of materials or apparatus.

Description

Technical field [0001] The invention belongs to the technical field of preparation of biomedical engineering materials, especially surface anticoagulant materials. Background technique [0002] For implants and interventional devices in contact with blood, such as extracorporeal circulation catheters, oxygenators, hemodialysis membranes, vascular stents and artificial blood vessels, it is a permanent issue to improve their surface antithrombotic performance. The most effective method is to modify the surface of the device material to improve the anticoagulant property of the stent. Because thrombin plays a crucial role in thrombus formation, most treatments aim to either block thrombin production or inhibit its activity. Heparin is widely used as an anticoagulant in clinical practice. Its anticoagulant mechanism is to inactivate thrombin or coagulation factors by binding to antithrombin III. The surface of the material is widely used to improve its anticoagulant properties...

Claims

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Application Information

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IPC IPC(8): A61L33/12
Inventor 王进杨志禄吕磊周烁黄楠
Owner SOUTHWEST JIAOTONG UNIV
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