Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin

A filter cup and bacteria technology, applied in the bacterial filter cup and its application field in the detection of antibacterial activity of bio-control bacterial metabolites, can solve the problem of inconvenient operation, affecting the reliability and authenticity of test results, and affecting the bio-control bacteria Problems such as the direction and amount of diffusion of the fermentation broth

Inactive Publication Date: 2012-10-03
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

(1) Bacterial filter sterilization method: After high-speed centrifugation of the bio-control bacterial fermentation liquid, take the supernatant and filter it with a bacterial filter. It can be used only after the sterility test, which is inconvenient to operate and the cost of the bacterial filter is high. , when the antibacterial activity of the fermented liquid is detected by the drug-containing plate method, more sterile filtrate is often required, and the sterile filtrate Oxford cup method is used. Although the amount of sample added is small, it is affected by the bottom and wall of the tube. It may affect the diffusion direction and diffusion amount of the biocon...

Method used

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  • Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin
  • Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin
  • Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin

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preparation example Construction

[0024] A method for preparing a bacterial filter cup and its application in the detection of antibacterial activity of bio-control bacterial metabolites, characterized in that: the operation is performed according to the following steps:

[0025] (1) Take a sterilized large glass plate with a diameter of 89 mm, and make a 1.8% water agar plate in it, and the thickness of the water agar plate is 3 mm;

[0026] (2) Take a cylindrical stainless steel cup body with a filter net at the bottom, the bottom of the cylindrical stainless steel cup body is provided with a circle of support feet with a height of 3mm, and place the cylindrical stainless steel cup body in the water of step (1). On the agar plate, press slightly so that the support feet at the bottom of the cup touch the bottom of the large glass plate, where the diameter of the large glass plate is larger than the diameter of the cylindrical stainless steel cup body;

[0027] (3) Inject water agar at a temperature of 50-60°...

Embodiment 1

[0037] Example 1: Ink tracking and color development observation

[0038] 1) Materials and methods:

[0039]Take a large glass plate with a diameter of 89mm to make a PSA medium plate, and use a medium puncher to punch a round hole with a diameter of 50mm on the PSA medium plate, remove the medium in the hole and inject 2.5ml of 15% ink into the hole , at room temperature, after 4h, 6h, 15h, and 24h, observe the diffusion of the ink in the PSA medium plate at fixed points, and measure the ink diffusion distance;

[0040] Simultaneously adopt the bacterial filter cup detection method of the present invention, the size of the bacterial filter cup is as follows: the diameter of the cylindrical cup body is 75mm, and the bottom of the cup of the cylindrical cup body has a diameter of 55mm round hole, and the round hole is provided with a diameter of 55mm. There is a layer of filter screen, the screen aperture of the filter screen is 1mm, the filter screen is provided with a water ...

Embodiment 2

[0050] Example 2: Antibacterial activity detection of metabolites of Bacillus subtilis

[0051] 1) Materials and methods

[0052] a. Tested strains and medium

[0053] Bacillus subtilis (Bacillus subtilis) T122F was selected as the biocontrol bacteria for testing, and Fusarium oxysporum f.sp.cubense (FO) was used as the indicator bacteria for the detection of antibacterial activity of bacterial metabolites. The width of the inhibition zone formed by confrontation culture in the PSA plate can reach 12mm.

[0054] First, liquid culture of Bacillus subtilis T122F was carried out in various mediums, and the medium used in this experiment was as follows:

[0055] NB medium: glucose 2.5g, beef extract 3g, peptone 5g, pH=7, water 1000ml;

[0056] Soybean meal medium: glucose 5g, soybean meal 8g, pH=7, water 1000ml;

[0057] Maltose medium: 5g maltose, 3g beef extract, 5g peptone, pH=7, 1000ml water;

[0058] Corn flour medium: corn flour 5g, beef extract 3g, peptone 5g, pH=7, wa...

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Abstract

The invention relates to a bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin. According to the invention, the bacteria filtering cup which is simple in structure is designed according to principles that bacteria thallus is impermeable to an agar culture medium, but bacteria metabolin can disperse in the agar culture medium and bacteria fermentation liquor is impermeable to the agar culture medium, and the bacteria filtering cup is applied to bacteriostatic activity detection of metabolin in the biocontrol bacteria fermentation liquor. The bacterial filtering cup is suitable for bacteriostatic activity detection of the biocontrol bacteria fermentation liquor in the development process of biopesticides, screening of bacterial strain culturing formulas, optimization of fungicide production process and stability detection of bacteriostatic active substances of biopesticide materials.

Description

technical field [0001] The invention relates to a simple bacterial filter cup in the development of biological pesticides and its application in the detection of biological control bacterial metabolites and biological pesticide antibacterial activity. Background technique [0002] In recent years, beneficial microorganisms and their metabolites have been widely used at home and abroad to inhibit the survival and activities of pathogens, which has led to the rapid development of biological control, among which biocontrol bacteria have played a very important role in the control of plant diseases. Antagonism is the main mechanism of action of bio-control bacteria, which refers to the antibacterial substances that bio-control bacteria can produce and secrete into the environment during their metabolism. Generally, at low concentrations, they can inhibit the growth and metabolism of pathogenic bacteria, and even kill them. dead pathogens. Such antibacterial substances mainly in...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12Q1/18
CPCC12M21/02C12M23/38C12M33/14
Inventor 杨秀娟陈福如何玉仙甘林石妞妞
Owner INST OF PLANT PROTECTION FAAS
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