Bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin

Abstract

The invention relates to a bacteria filtering cup and application thereof to bacteriostatic activity detection of biocontrol bacteria metabolin. According to the invention, the bacteria filtering cup which is simple in structure is designed according to principles that bacteria thallus is impermeable to an agar culture medium, but bacteria metabolin can disperse in the agar culture medium and bacteria fermentation liquor is impermeable to the agar culture medium, and the bacteria filtering cup is applied to bacteriostatic activity detection of metabolin in the biocontrol bacteria fermentation liquor. The bacterial filtering cup is suitable for bacteriostatic activity detection of the biocontrol bacteria fermentation liquor in the development process of biopesticides, screening of bacterial strain culturing formulas, optimization of fungicide production process and stability detection of bacteriostatic active substances of biopesticide materials.

Description

technical field [0001] The invention relates to a simple bacterial filter cup in the development of biological pesticides and its application in the detection of biological control bacterial metabolites and biological pesticide antibacterial activity. Background technique [0002] In recent years, beneficial microorganisms and their metabolites have been widely used at home and abroad to inhibit the survival and activities of pathogens, which has led to the rapid development of biological control, among which biocontrol bacteria have played a very important role in the control of plant diseases. Antagonism is the main mechanism of action of bio-control bacteria, which refers to the antibacterial substances that bio-control bacteria can produce and secrete into the environment during their metabolism. Generally, at low concentrations, they can inhibit the growth and metabolism of pathogenic bacteria, and even kill them. dead pathogens. Such antibacterial substances mainly in...

AI Technical Summary

Problems solved by technology

(1) Bacterial filter sterilization method: After high-speed centrifugation of the bio-control bacterial fermentation liquid, take the supernatant and filter it with a bacterial filter. It can be used only after the sterility test, which is inconvenient to operate and the cost of the bacterial filter is high. , when the antibacterial activity of the fermented liquid is detected by the drug-containing plate method, more sterile filtrate is often required, and the sterile filtrate Oxford cup method is used. Although the amount of sample added is small, it is affected by the bottom and wall of the tube. It may affect the diffusion direction and diffusion amount of the biocontrol bacterial fermentation liquid. The antibacterial plaques formed during the bioassay are not necessarily regular, and it is impossible to quantitatively distinguish the fermentation liquid with the same inhibition zone size and different clarity; (2) high temperature Sterilization method: It may lead to the inactivation of some antibacterial metabolites in the fermentation liquid that are not resistant to high temperature, which will affect the reliability and authenticity of the test results; A polar antibacterial active substance, the operation process is relatively complicated, and usually requires a large amount of fermentation broth and organic solvents for extraction

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