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Improved preparation method of kelp chromosome

A chromosome and kelp technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of unpopular use and high sales price of fluorescence microscope, and achieve the effect of strong repeatability, simple and fast method

Active Publication Date: 2012-10-03
SHANDONG ORIENTAL OCEAN SCI TECH
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  • Summary
  • Abstract
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  • Claims
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Problems solved by technology

However, dyeing with fluorescent dyes can only be observed through a fluorescent microscope, and the market price of fluorescent microscopes is relatively high, so it cannot be widely used

Method used

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  • Improved preparation method of kelp chromosome
  • Improved preparation method of kelp chromosome
  • Improved preparation method of kelp chromosome

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Embodiment Construction

[0025] specific implementation plan

[0026] Below in conjunction with accompanying drawing and embodiment describe in detail:

[0027] 1. The kelp gametophytes in this example were obtained from the "Dongfang No. 2" hybrid kelp gametophytes preserved in the germplasm preservation bank of the National Seaweed Engineering Technology Research Center of Shandong Oriental Ocean Technology Co., Ltd.

[0028] Kelp gametophyte can also be collected according to the following method: in the season of kelp maturation, select robust sporophyte, take off the kind of kelp block with young sporangia, and use sterilized seawater (boil raw seawater for 2 minutes to obtain sterilized seawater) and sterilized cotton (The degreased cotton is wrapped in kraft paper and placed in a pressure steam sterilizer at a temperature of 120°C and a pressure of 0.165MPa for 15 minutes to obtain sterilized cotton.) Scrub multiple times to remove attachments and algae. After the kelp pieces are dried in the s...

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PUM

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Abstract

The invention discloses an improved preparation method of a kelp chromosome. The steps are absorbing surplus moisture after kelp gamobiums are centrifuged, keeping the kelp gamobiums to be deposited, and adding colchicines solution for evenly shaking and stewing; conducting hypotension on the kelp gamobiums by using potassium chloride solution, fixing the kelp gamobiums by sing kano stationary liquid after hypotension, conducting enzymolysis and wall removal through mixed enzyme liquid of cellulose R-10 solution, pectinase solution and liquation enzyme R-10 solution, absorbing supernate by using a liquor relief gun after the kelp gamobiums are centrifuged, adding glacial acetic acid for evenly mixing to form cell suspension, absorbing the cell suspension by using a dropper to an upper dropping sheet of a centrifuging and pre-cooling glass slide, burning the bottom face of the glass slide by using an alcohol burner for one time, and naturally drying or stoving, dying the kelp gamobium chromosome adhered to the glass slide by using giemsa staining fluid, and observing and photographing under a microscope. The chromosomes obtained by using the improved preparation method are good in segregation, differentiable in shape and denumerable in chromosome split pahse, and the improved preparation method is simple and rapid in method, strong in repeatability and capable of opening in a general laboratory.

Description

technical field [0001] The invention relates to a method for preparing chromosomes, in particular to an improved method for preparing kelp chromosomes. Background technique [0002] The sporophyte of kelp is large and is the object of artificial cultivation. Laminaria gametophytes are tiny filamentous bodies, which are the object of artificial cultivation of seedlings and the basis for the preservation of kelp germplasm resources. Laminaria gametophytes are divided into male and female. The female gametophyte is composed of one or several cells, and the male gametophyte is generally composed of multiple cells. The male gametophyte cells are smaller than the female gametophyte cells. During the growth process, the female gametophytes increase the volume of the cells, while the male gametophytes undergo cell division, increase the number of cells, and form multicellular branches or spheroids. [0003] Laminaria chromosomes are small, and the number of chromosomes in Laminari...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 田萍萍崔翠菊张立楠李晓捷罗世菊赵楠宋少峰赛珊盛宝利彭伟吴家奇邵伟
Owner SHANDONG ORIENTAL OCEAN SCI TECH
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