Human serum protein concentration testing device and method

Abstract

The invention discloses a human serum protein concentration testing device and method. The device consists of a light-emitting diode array, a micro-lens array, an optical filter, a converging lens, a sample pond, an optical fiber light cone, a narrowband light filtering film, a power supply, an SSPM (Security Service Provider Module) detector, a signal processing circuit and a display module. The method comprises the following steps of: collimating light generated by the micro-lens array, filtering, converging and emitting into Zn(II) tetracarboxy phthalocyanine and a serum protein reaction solution to be detected in the sample pond; activating the reaction solution to generate fluorescent light by using a light source; acquiring and transmitting fluorescent light by using the optical fiber light cone; filtering background parasitic light by using the narrowband light filtering film; performing photoelectric conversion on a light signal by using the SSPM detector; and processing a signal output by the SSPM detector by using the signal processing circuit, and displaying the concentration value of serum proteins on a liquid display screen of the display module. The testing device disclosed by the invention has the advantages of small size, low cost, convenience for carrying and high sensitivity; and the testing method is easy to operate, and contributes to popularizing.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a human serum protein concentration testing device and a testing method. Background technique [0002] Protein is the source of all life and one of the most basic substances in life activities. It has a large content and a wide variety in organisms, and it carries the tasks of organisms to complete various biological functions, which is very important and indispensable for maintaining life. In a normal human body, protein accounts for 18%. The skin, muscle, blood, and internal organs of the human body are mainly composed of protein. Hair, bones, nails, and various hormones, enzymes, and antibodies in the body are all proteins. Proteins are responsible for various physiological functions. Maintaining the metabolic activities of organisms as a whole is the direct expression of biological traits. For example, human serum albumin (HSA) is the most abundant tran...

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Problems solved by technology

[0004] At present, there are a variety of measuring instruments for the concentration of serum protein solutions at home and abroad, such as the Hemoglobin serum protein measuring instrument in Sweden, which mainly uses the reagent strip method to measure protein concentration. This instrument is convenient for blood collection, does not need to be diluted, and the test is simple and very applicable. The test is carried out at the athlete training site, but its price is so high that small and medium-sized hospitals cannot purchase in batches. In addition, the reagent strips used are also expensive, and they are one-time consumables, which undoubtedly increases the burden on patients; domestic companies such as Shanghai Precision Scientific Instrument Co., Ltd. The 5200-type hemoglobin meter produced by the company's analysis factory uses a single-wavelength method to let light of a certain wavelength pass through a cuvette filled with a blank solution (high iron cyanide standard dilution), and then let the light of the same wavelength pass through a cuvette filled with cyanide The cuvette of the hemoglobin reaction solution generated by the reaction of high iron and the diluted hemoglobin solution is used to analyze the two light intensities after passing through the cuvette with the blank solution and the cuvette with the hemoglobin reaction solution respectively, so as to obtain The concentration of the measured solution, this instrument has a short measurement time, but it needs an external diluter to dilute the blood 250 times, and then hemolyzes the diluted blood with ferric cyanide as the measured solution, resulting in the The volume is very large, and it takes a long time to dilute and hemolyze at the same time, which greatly increases the time for the actual measurement of a single blood sample; Calibration leads to complicated operation and low measurement efficiency; in addition, the final measurement result is easily affected by external factors (such as the operator's skill level, etc.) due to two measurements, which directly affects the measurement accuracy

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