Preparation method for manufacturing silkworm pupa protein of protein filament

A technology of silkworm chrysalis protein and silkworm chrysalis protein powder, which is applied to the preparation method of peptides, chemical instruments and methods, peptides, etc. It can solve the problems of broken silkworm chrysalis protein filaments, recovery of silkworm chrysalis protein peptides, and low extraction rate of products. The effect of high quality, low production cost and simple production process

Inactive Publication Date: 2013-10-02
成都起点投资有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: microwave treatment cannot break the chemical connection between globulin and chitin, and the extracted silkworm chrysalis protein is a compound of silkworm chrysalis protein and chitin, which may cause allergic reactions when used as food additives; silkworm chrysalis prepared with it The protein alkali solution is peptone at room temperature, which blocks the spinning hole, resulting in the breakage of silkworm chrysalis protein fibers, which seriously affects the quality of spinning; the utilization rate of raw materials is low, only silkworm chrysalis protein is extracted, and the silkworm chrysalis protein peptides that may be produced after the hydrolysis reaction are not affected. etc. for recycling; the product extraction rate is low, and the mass fraction of silkworm chrysalis protein powder extracted is only 85%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing silkworm pupa protein for producing protein filaments, the specific steps of the method are as follows:

[0026] (1) Preparation of alkaline solution of defatted silkworm pupa powder

[0027] Using commercially available defatted silkworm pupa as raw material, first send the defatted silkworm pupa into a pulverizer for pulverization, then collect the powder and pass through a 20-mesh sieve for separation, and collect the sieved powder (that is, the prepared defatted silkworm pupa powder); The crude product is returned to the crusher and then crushed. Then send the collected defatted silkworm pupa powder into the batching kettle, according to the mass fraction of defatted silkworm pupa powder (kg): the mass fraction of sodium hydroxide is 0.1% sodium hydroxide aqueous solution volume (L) ratio is 1:8 Proportion, the sodium hydroxide aqueous solution is pumped into the batching kettle, stirred and mixed evenly, and the alkaline solution of defatted silk...

Embodiment 2

[0037] A preparation method of silkworm pupa protein for producing protein filaments is the same as in Example 1, wherein:

[0038] In step (1), the powder is collected and separated through a 40-mesh sieve. The ratio of the mass of defatted silkworm pupa powder (kg): the volume of sodium hydroxide aqueous solution (L) with a mass fraction of sodium hydroxide of 1% is In the ratio of 1:12, the sodium hydroxide aqueous solution is pumped into the batching kettle.

[0039] In step (2), water vapor with a pressure of 0.25 MPa is introduced into the interlayer of the glass-lined reactor to heat the alkaline solution of defatted silkworm pupa powder in the glass-lined reactor to 75° C. and keep it for 0.75 h. Under stirring, continue to pass in water vapor to continue heating to 107°C, and keep the temperature for 1 hour before turning off the water vapor. Then, the mixed solution of the broken silkworm pupa globulin and chitin compound in the glass-lined reactor was cooled to 30°C und...

Embodiment 3

[0044] A preparation method of silkworm pupa protein for producing protein filaments is the same as in Example 1, wherein:

[0045] In step (1), the powder is collected and separated through a 60-mesh sieve. The ratio of the mass of defatted silkworm pupa powder (kg): the volume of sodium hydroxide aqueous solution (L) with a mass fraction of sodium hydroxide of 1% is The ratio of 1:16, the sodium hydroxide aqueous solution is pumped into the batching kettle.

[0046] In step (2), water vapor with a pressure of 0.4 MPa is introduced into the interlayer of the glass-lined reactor to heat the alkaline solution of defatted silkworm pupa powder in the glass-lined reactor to 80° C. and keep it for 1 hour. Under stirring, continue to pass in water vapor to continue heating to 115°C, and keep the temperature for 1.5 hours before turning off the water vapor. Then, the mixed solution of the broken silkworm pupa globulin and chitin compound in the glass-lined reactor was cooled to 20°C unde...

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Abstract

The invention relates to a preparation method for manufacturing silkworm pupa protein of protein filaments, relating to a method for extraction of silkworm pupa protein from silkworm pupa. The defatted silkworm pupa available commercially is used as a raw material, and the product is obtained via preparation of alkaline solution of defatted silkworm pupa powder, chitin-free silkworm pupa protein solution and precipitation solution thereof and silkworm pupa protein powder for filaments and recycling of silkworm pupa protein peptide. The method is simple, the production equipment is conventional general equipment, the energy consumption is low, the cost is low, and the promotion and use can be facilitated; and high-quality silkworm pupa protein, silkworm pupa protein peptide and other products can be produced according to the method disclosed by the invention, the silkworm pupa can be comprehensively and fully utilized, no ''three-waste'' emissions can be produced, the environmental protection can be facilitated, and the method belongs to a preparation method of green bio-chemical products. The method can be widely applied in preparation of silkworm pupa protein and silkworm pupa protein peptide. The silkworm pupa protein prepared in the method can be applied in manufacturing of adhesive protein filaments, food additives, bio-chemical culture mediums for fermentation, feed additives and the like; and the prepared silkworm pupa protein peptide can also be used as a high-quality nutritional agent for animal cubs.

Description

1. Technical Field [0001] The invention belongs to the technical field of animal protein preparation. It specifically relates to a method for extracting silkworm pupa protein from silkworm pupa. 2. Background technology [0002] Silkworm pupa is the main by-product of the silk reeling industry. Every 1 ton of raw silk produced can produce nearly 1 ton of dry silkworm pupa by-products. China is the most important silk producer in the world. According to statistics, my country can produce more than 200,000 tons of dried silkworm pupa by-products each year. According to measurements, dried silkworm pupae contain about 48%-60% crude protein, 25%-31% fatty acids, 2%-5% carbohydrates, and 2%-3% chitin. Silkworm pupa protein is a kind of high-quality pure natural full-price protein. Its efficacy ratio and biological value are significantly better than soy protein, and it is easy to be digested and absorbed by the human body, and has the effect of promoting animal growth and developmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C07K1/14
Inventor 孙烽周小华余文琴蔡怀德冯海燕
Owner 成都起点投资有限公司
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