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Traditional Chinese medicinal composition used for postoperative adjuvant therapy for colorectal cancer

A technology of adjuvant therapy, composition, applied in the field of traditional medicine application

Inactive Publication Date: 2012-10-17
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The technical problem to be solved by the present invention is to find a traditional Chinese medicine composition that has a therapeutic effect on colorectal cancer after operation and can significantly reduce adverse reactions according to traditional medical theory.

Method used

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  • Traditional Chinese medicinal composition used for postoperative adjuvant therapy for colorectal cancer
  • Traditional Chinese medicinal composition used for postoperative adjuvant therapy for colorectal cancer
  • Traditional Chinese medicinal composition used for postoperative adjuvant therapy for colorectal cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Experimental Study on Inhibiting Proliferation of Human Colon Cancer SW480 Cells

[0016] 1.1 Main experimental materials

[0017] 1.1.1 Experimental cells

[0018] Human colon cancer cell SW480: purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences. Cultivate according to conventional methods, that is, with 10% fetal bovine serum, 100U·mL -1 Penicillin, 100U·mL -1 Streptomycin in RPMI 1640 medium at 37°C, 5% CO 2 cultured in an incubator. Cells in logarithmic growth phase were used for experiments.

[0019] 1.1.2 Drugs and Reagents

[0020] The traditional Chinese medicine composition for postoperative adjuvant treatment of colorectal cancer (hereinafter referred to as FFCT), consists of 500g ginseng, 900g diffusa, 500g curcuma, 150g dung beetle, 500g August fried, 2.5g crude drug·mL -1 , batch number 20071112;

[0021] 5-Fluorouracil (5-Fu): Tianjin Jinyao Amino Acid Co., Ltd., 0.025g mL -1 , batch number 0710052;

[0022] RPMI 1640 ...

Embodiment 2

[0106] Mechanism of FFCT promoting apoptosis of human colon cancer cells SW480 through caspase-3 pathway

[0107] 2.1 Main experimental materials

[0108] 2.1.1 Experimental cells

[0109] Human colon cancer cell SW480: purchased from Shanghai Institute of Cell Biology, Chinese Academy of Sciences. Cultivate according to conventional methods, that is, with 10% fetal bovine serum, 100U·mL -1 Penicillin, 100U·mL -1 Streptomycin in RPMI 1640 medium at 37°C, 5% CO 2 cultured in an incubator. Cells in logarithmic growth phase were used for experiments.

[0110] 2.1.2 Drugs and reagents

[0111] FFCT, consisting of 700g ginseng, 1200g Hedyotis diffusa, 700g Curcuma zedoary, 250g dung beetle, 700g fried in August: batch number 20071112;

[0112] 5-Fluorouracil (5-Fu): Tianjin Jinyao Amino Acid Co., Ltd., 0.025g mL -1 , batch number 0710052;

[0113] Caspase-3 inhibitor Ac-DEVD-CHO: Biyuntian Biotechnology Research Institute;

[0114] RPMI 1640 medium: Gibco company;

[011...

Embodiment 3

[0178] Effects of FFCT on the immune function of H22 tumor-bearing mice

[0179] 3.1 Main experimental materials

[0180] 3.1.1 Experimental animals

[0181] Clean-grade Kunming mice: purchased from Shanghai Slack Experimental Animal Co., Ltd. 40 animals, half male and half female, weighing 18-22g, animal certificate number: SCXK (Shanghai) 2007-0005; experimental animal use certificate number: SYXK (Su) 2007-0026.

[0182] 3.1.2 Drugs and reagents

[0183] FFCT prescription, composed of 600g ginseng, 1100g diffusa, 600g curcuma, 200g dung beetle, 600g August fried, made into granules, batch number 20071112;

[0184] Cyclophosphamide powder injection (Cy): 0.2g bottle -1 , Jiangsu Hengrui Pharmaceutical Co., Ltd., batch number: 07020121;

[0185] Yangxueyin oral liquid (YXY): 10mL bottle -1 , Jilin Province Liyuan Pharmaceutical Co., Ltd., batch number: 070926;

[0186] Physiological saline for injection: Nanjing Xiaoying Pharmaceutical Group Co., Ltd., batch number 200...

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Abstract

The invention belongs to the field of application of traditional medicines and develops a traditional Chinese medicinal composition used for postoperative adjuvant therapy for colorectal cancer and capable of reducing side effects of chemotherapeutics by using the theory of traditional Chinese medicines on the basis of long-term clinical practice. A technical scheme provided in the invention is as follows: 500 to 700 g of the Chinese herbal medicine of ginseng, 900 to 1200 g of heydyotis, 500 to 700 g of curcuma rhizome, 150 to 250 g of aspongopus and 500 to 700 g of akebia fruit are selected and used to prepare particles of the traditional Chinese medicinal composition. A recipe mentioned-above is a proved clinical recipe obtained under the guidance of the theory of traditional Chinese medicines and has a good curative effect according to results of years of clinical practice; according to statistical analysis results of data of modern pharmacological and pathological experiments, the recipe has a curative effect in postoperative adjuvant therapy for colorectal cancer indeed.

Description

【Technical field】 [0001] The invention belongs to the application field of traditional medicine, which utilizes traditional Chinese medicine theory and finds out a traditional Chinese medicine composition on the basis of long-term clinical practice for adjuvant treatment of colorectal cancer after surgery, and can also reduce the side effects of chemotherapy drugs. 【Background technique】 [0002] At present, surgery is the first choice for the treatment of colorectal cancer, but colorectal cancer has a high postoperative metastasis and recurrence rate, except for some early-stage patients, all need chemotherapy. Although chemotherapy can eliminate the micrometastasis in the patient's body and increase the chance of cure, chemotherapy drugs can also damage normal cells while killing tumor cells. Therefore, it is urgent to use traditional medical theory to find a traditional Chinese medicine that can treat colorectal cancer after surgery. At the same time, it can also signific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9066A61K9/16A61P35/00A61P37/04
Inventor 王小宁霍介格李灵常黄一平丁蓉丁永芳沈明勤
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE