Application of chitosan serving as immuno-adjuvant in preparing mouse allergic asthma model

Abstract

The invention belongs to the technical field of biology, and relates to an allergic asthma model, in particular to application of chitosan serving as immuno-adjuvant in preparing a mouse allergic asthma model. According to the application, the mouse allergic asthma model prepared by applying chitosan serving as immuno-adjuvant is subjected to airway responsiveness, lung tissue pathological examination and inflammation marker inspection, and results indicate that the mouse allergic asthma model prepared by applying chitosan serving as immuno-adjuvant is remarkably superior to a conventional immuno-adjuvant prepared asthma model in airway eosinophilic inflammation, airway responsiveness and airway inflammatory cytolines and other aspects. The chitosan has low price and rich sources; and the mouse asthma model by using chitosan as immuno-adjuvant is remarkably superior to a traditional adjuvant, and has the advantages of simple method, low cost, good reproducibility and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an allergic asthma model, in particular to the use of chitosan as an immune adjuvant in preparing a mouse allergic asthma model. Background technique [0002] Bronchial asthma (asthma) is a clinical common disease and frequently-occurring disease, and the incidence rate is on the rise in recent years. Asthma is a chronic airway allergic inflammatory disease involving a variety of inflammatory cells such as eosinophils, mast cells, and T lymphocytes. Its main manifestations are airway hyperresponsiveness, reversible airflow limitation, and mucus Hypersecretion, airway remodeling can also occur in the late stage. Its pathogenesis has not been fully elucidated. At present, it is believed that the excessive activation of Th2 cells leads to a Th2-based immune response, increased secretion of Th2 cytokines IL-4, IL-5, IL-13, etc., and eventually leads to the accumulation of inflammatory ce...

AI Technical Summary

Problems solved by technology

[0004] But there are two problems in this method: (1) the imported liquid aluminum hydroxide gel is expensive, and the effect of using common aluminum hydroxide solution as an adjuvant is poor, and the airway eosinophilic inflammation is not obvious; The airway responsiveness and airway inflammatory cytokines of the asthmatic mouse model induced by the gel as an adjuvant are not significantly increased, and are unstable, and often have no significant difference or no significant difference with normal mice, resulting in the model Construction failure, affecting the efficacy evaluation of therapeutic drugs

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