Application of chitosan serving as immuno-adjuvant in preparing mouse allergic asthma model

A technology of allergic asthma and chitosan, which is used in allergic asthma models. Chitosan is used as an immune adjuvant in the field of preparing mouse allergic asthma models, which can solve the airway reactivity and airway inflammation in asthma mouse models. It can improve the effect of enhancing the cytotoxic T cell response, improving the uptake rate and utilization rate, and enhancing the delayed allergic reaction.

Inactive Publication Date: 2012-10-17
THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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Problems solved by technology

[0004] But there are two problems in this method: (1) the imported liquid aluminum hydroxide gel is expensive, and the effect of using common aluminum hydroxide solution as an adjuvant is poor, and the airway eosinophilic inflammation is not obvious; The airway responsiveness and airway inflammatory cytok

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  • Application of chitosan serving as immuno-adjuvant in preparing mouse allergic asthma model
  • Application of chitosan serving as immuno-adjuvant in preparing mouse allergic asthma model
  • Application of chitosan serving as immuno-adjuvant in preparing mouse allergic asthma model

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Embodiment Construction

[0027] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. Unless otherwise defined, all professional and scientific terms used herein have the same meanings as commonly understood by those skilled in the art. In addition, any methods and materials similar or equivalent to those described can also be applied in the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only.

[0028] 1. Materials and methods

[0029] 1. Establish mouse allergic asthma model (model 1) induced by chitosan adjuvant and chicken ovalbumin

[0030] Chitosan is from Qingdao Haihui Company, with a molecular weight of 100,000 Daltons and a deacetylation degree of 95%. The chitosan is dissolved in 1% acetic acid aqueous solution to prepare a ch...

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Abstract

The invention belongs to the technical field of biology, and relates to an allergic asthma model, in particular to application of chitosan serving as immuno-adjuvant in preparing a mouse allergic asthma model. According to the application, the mouse allergic asthma model prepared by applying chitosan serving as immuno-adjuvant is subjected to airway responsiveness, lung tissue pathological examination and inflammation marker inspection, and results indicate that the mouse allergic asthma model prepared by applying chitosan serving as immuno-adjuvant is remarkably superior to a conventional immuno-adjuvant prepared asthma model in airway eosinophilic inflammation, airway responsiveness and airway inflammatory cytolines and other aspects. The chitosan has low price and rich sources; and the mouse asthma model by using chitosan as immuno-adjuvant is remarkably superior to a traditional adjuvant, and has the advantages of simple method, low cost, good reproducibility and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an allergic asthma model, in particular to the use of chitosan as an immune adjuvant in preparing a mouse allergic asthma model. Background technique [0002] Bronchial asthma (asthma) is a clinical common disease and frequently-occurring disease, and the incidence rate is on the rise in recent years. Asthma is a chronic airway allergic inflammatory disease involving a variety of inflammatory cells such as eosinophils, mast cells, and T lymphocytes. Its main manifestations are airway hyperresponsiveness, reversible airflow limitation, and mucus Hypersecretion, airway remodeling can also occur in the late stage. Its pathogenesis has not been fully elucidated. At present, it is believed that the excessive activation of Th2 cells leads to a Th2-based immune response, increased secretion of Th2 cytokines IL-4, IL-5, IL-13, etc., and eventually leads to the accumulation of inflammatory ce...

Claims

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Application Information

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IPC IPC(8): A61K39/39
Inventor 王彦王长征毕玉田卓文磊林科雄张巧
Owner THE SECOND AFFILIATED HOSPITAL ARMY MEDICAL UNIV
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