Method for preparing salvianolic acid A bulk drug

A technology for salvianolic acid and salvia miltiorrhiza, applied in the field of medicine, can solve the problems of complex extraction and separation steps, high production cost, unsuitable for industrialized production and the like

Active Publication Date: 2012-10-17
INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Compared with other water-soluble phenolic acids in Salvia miltiorrhiza, such as danshensu, rosmarinic acid, and salvianolic acid B, which have higher content in medicinal materials and relatively easy extraction and separation, the salvianolic acid in Danshen medicinal materials from different origins The content of A is only about 1 to 3 / 10,000 molecules, and the extraction and separation steps are complicated, and the production yield is low. Therefore, researchers have been working on finding an efficient method for preparing high-purity salvianolic acid A for many years.
For example, Chinese patent applications CN1397276, CN1513848, CN1830947A, CN1887849A, and CN101480423A all involve the preparation of salvianolic acid A, but the preparation methods in the above-mentioned patents have complicated technological processes and high production costs, and are not suitable for industrial production or the preparation purity cannot reach Insufficient requirements for raw materials

Method used

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  • Method for preparing salvianolic acid A bulk drug
  • Method for preparing salvianolic acid A bulk drug
  • Method for preparing salvianolic acid A bulk drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Grind the dried root of Salvia miltiorrhiza, add 6 times of water to reflux and extract 4 times, the time of each extraction is 4, 3, 2, 1 hour in turn, combine the extracts, filter, concentrate under reduced pressure, so that the specific gravity of the concentrate is 1.20g / ml, add 95% ethanol, stir while adding until the ethanol concentration is 50%, let it stand overnight, take the supernatant, concentrate under reduced pressure until there is no ethanol smell, adjust the pH value to 4.5 with concentrated hydrochloric acid, and use an equal amount of acetic acid Ethyl ester was extracted 4 times, the extracts were combined and recovered under reduced pressure to obtain Extract I. Dissolve the extract I with 10% ethanol, absorb it on a D101 macroporous resin chromatographic column, elute with 10%, 30%, 50%, 70%, 95% ethanol respectively, and collect the eluate containing salvianolic acid A , concentrated under reduced pressure to obtain a concentrated solution rich i...

Embodiment 2

[0032] Grind the dried root of Salvia miltiorrhiza, add 10 times of 50% ethanol to reflux and extract 3 times, the time of each extraction is successively 4, 3, 2 hours, combine the extracts, filter, concentrate under reduced pressure, so that the specific gravity of the concentrate is 1.3g / ml, add 95% ethanol, stir while adding until the ethanol concentration is 70%, let it stand overnight, take the supernatant, concentrate under reduced pressure until there is no ethanol smell, adjust the pH value to 4.0 with concentrated sulfuric acid, and use an equal amount of two Chloromethane was extracted 4 times, the extracts were combined, recovered under reduced pressure, and the extract I was obtained. The extract I was dissolved in 20% methanol, adsorbed on the AB-8 type macroporous resin chromatographic column, and eluted with 20%, 40%, 60%, 80%, 95% methanol respectively, and the elution containing salvianolic acid A was collected. Deliquoring, concentrating under reduced press...

Embodiment 3

[0034] Grind the dried root of Salvia miltiorrhiza, add 12 times of 80% methanol to reflux and extract 3 times, the time of each extraction is 4, 3, 2 hours successively, combine the extracts, filter, and concentrate under reduced pressure, so that the specific gravity of the concentrate is 1.05g / ml, add 95% ethanol, stir while adding until the ethanol concentration is 80%, let it stand overnight, take the supernatant, concentrate under reduced pressure until there is no ethanol smell, adjust the pH value to 2.5 with phosphoric acid, and use an equal amount of trichloro Methane was extracted 4 times, the extracts were combined, and recovered under reduced pressure to obtain Extract I. Extract I was dissolved with 15% acetone, adsorbed on HP-20 type macroporous resin chromatographic column, eluted with 15%, 35%, 55%, 75%, 95% acetone respectively, and collected the elution containing salvianolic acid A. Deliquoring, concentrating under reduced pressure to obtain a concentrated...

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Abstract

The invention relates to a preparation method for a salvianolic acid A bulk drug and its medicinal preparation. The method comprises the following steps: employing dried roots of Salvia miltiorrhiza Bge as a raw material, crushing, extracting a solvent, extracting an organic solvent, carrying out macroporous resin or polyamide resin chromatographic column chromatography, extracting the organic solvent, drying and the like to obtain the salvianolic acid A. Compared with the prior arts for preparing the salvianolic acid A, the method of the invention has the characteristics of simple process flow, low cost, high product purity, good industrial adaptability and the like. The salvianolic acid A prepared by the invention can be prepared to pharmaceutically acceptable medicinal preparation forms of tablets, capsules, particulate agents, oral liquids, dripping pills, pellets, injection and the like.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a method for extracting salvianolic acid A from traditional Chinese medicine Salvia miltiorrhiza and a preparation method for a pharmaceutical preparation containing the same. Background technique [0002] Salvianolic acid A (Salvianic acid A) is a water-soluble phenolic acid compound contained in the dry root of Salvia miltiorrhiza Bunge, which was first discovered by Professor Li Lianniang, Institute of Materia Medica, Chinese Academy of Medical Sciences in 1984. It is isolated from Salvia miltiorrhiza (Li Lian-Niang, et al. Planta Medica. 1984, 50: 227). In recent years, a large number of pharmacological tests have shown that salvianolic acid A has strong myocardial ischemia protection (Du Guanhua, etc. Acta Pharmacol. 1995, 30 (10): 731; Wang Shou-Bao, et al.Eur.J.Pharmacol .2009, 615: 125), antithrombotic (Liu Yinglin, et al. Acta Pharmaceutica Sinica. 2002, 37(2): 81; F...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/732C07C67/58A61K31/216A61P9/10A61P7/02A61P25/00A61P3/10
Inventor 杜冠华张维库张莉赵莹王夙博杨海光田硕
Owner INST OF MATERIA MEDICA CHINESE ACAD OF MEDICAL SCI
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