Visual rapid combined measuring method of plant pathogen and antibody chip

A plant pathogen and antibody chip technology, applied in the biological field, can solve the problems of large loss of target nucleic acid, huge workload, long detection time, etc., and achieve the effects of saving antibodies, broad prospects for promotion, and easy operation.
CN102735836AInactive Publication Date: 2012-10-17上海慧耘生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
上海慧耘生物科技有限公司
Publication Date
2012-10-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a visual rapid combined measuring method of plant pathogen and an antibody chip. The method provided by the invention comprises the following steps of: distributing plant pathogen grabbing antibody points on basal lamina to obtain an antibody chip, hybridizing a detected sample and the antibody chip for incubation,washing, adding a corresponding plant pathogen detection antibody, continuously reacting, washing and developing. The method provided by the invention has the same sensitivity, specificity and reproducibility with methods such as ELISA and the like, and also has the capability of detecting a plurality of pathogens in one experiment, which is what traditional methods don't have.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a detection method and reagents for plant pathogens, especially a method for visual rapid joint detection of plant pathogens and an antibody chip. Background technique

[0002] Plant diseases are divided into two categories: infective and non-infectious, and infective diseases can be mainly divided into fungal, bacterial, viral and nematode. There are many kinds of plant diseases and wide transmission routes, which can destroy or interfere with the normal physiological functions of plants. At present, there is a lack of effective control measures, which makes it difficult to control many important viral and bacterial diseases once they are prevalent. One of the most important causes of quality degradation.

[0003] At present, the detection methods for plant diseases mainly include biological detection, electron microscope observation, serological detection and molecular biological ...

Claims

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