Visual rapid combined measuring method of plant pathogen and antibody chip

Abstract

The invention discloses a visual rapid combined measuring method of plant pathogen and an antibody chip. The method provided by the invention comprises the following steps of: distributing plant pathogen grabbing antibody points on basal lamina to obtain an antibody chip, hybridizing a detected sample and the antibody chip for incubation,washing, adding a corresponding plant pathogen detection antibody, continuously reacting, washing and developing. The method provided by the invention has the same sensitivity, specificity and reproducibility with methods such as ELISA and the like, and also has the capability of detecting a plurality of pathogens in one experiment, which is what traditional methods don't have.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a detection method and reagents for plant pathogens, especially a method for visual rapid joint detection of plant pathogens and an antibody chip. Background technique [0002] Plant diseases are divided into two categories: infective and non-infectious, and infective diseases can be mainly divided into fungal, bacterial, viral and nematode. There are many kinds of plant diseases and wide transmission routes, which can destroy or interfere with the normal physiological functions of plants. At present, there is a lack of effective control measures, which makes it difficult to control many important viral and bacterial diseases once they are prevalent. One of the most important causes of quality degradation. [0003] At present, the detection methods for plant diseases mainly include biological detection, electron microscope observation, serological detection and molecular biological ...

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Problems solved by technology

[0004] However, the above common detection methods for plant diseases have more or less defects: biological detection generally needs to be carried out in a greenhouse, and the whole detection process takes a long time; electron microscope observation generally requires negative staining or making ultra-thin sections , and the detection and identification process needs to be carried out under an electron microscope; serological methods such as ELISA have a long detection time, and each reaction well can only detect one pathogen alone, when it is necessary to screen a large number of samples or multiple pathogenic items in a single sample , the workload is often quite large, and has the disadvantages of time-consuming and laborious; molecular biology methods need to label probe molecules (nucleic acid molecular hybridization technology), or extract nucleic acid and further reverse-transcribe mRNA into cDNA (RC-PCR), detect During the process, the loss of target nucleic acid is relatively large, and the detection result may be false negative because the nucleic acid hybridization or amplification process is easily inhibited by plant components

At the beginning of the birth of the protein chip, it was mainly used for proteomics research, and then it was gradually extended to related fields of biomedicine. However, there are still no relevant reports at home and abroad on the use of protein chips (antibody chips) for the detection of plant pathogens.

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