Biologic graphite oxide obtained by nutrifying bacteria and preparation method for biologic graphite oxide

A technology of biological oxidation and nitrifying bacteria, applied in the direction of graphite, etc., can solve problems such as defects, carbon skeleton lattice distortion and distortion, carbon skeleton lattice distortion and distortion at the atomic level, and achieve good industrial application prospects and value. Preparation The process is green and pollution-free, and the effect of mild reaction conditions

Inactive Publication Date: 2013-09-11
NANJING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, strong acids and strong oxidants are used in the preparation of graphene oxide by chemical oxidation, which has the disadvantages of serious environmental pollution, and the introduction of oxygen-containing groups may easily lead to lattice distortion and distortion at the atomic level of the carbon skeleton, and generate defects (Florian B ., Jani K. and Arkady V. K. Structural Defects in Graphene. ACS NANO, 2011, 5(1): 26-41), the π-π conjugated bonds were largely destroyed, and the carbon skeleton produced an obvious lattice at the atomic level Distortion and distortion, loss of many excellent properties

Method used

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  • Biologic graphite oxide obtained by nutrifying bacteria and preparation method for biologic graphite oxide
  • Biologic graphite oxide obtained by nutrifying bacteria and preparation method for biologic graphite oxide
  • Biologic graphite oxide obtained by nutrifying bacteria and preparation method for biologic graphite oxide

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Embodiment 1

[0029] Embodiment 1: A preparation method of bio-oxidized graphite according to the present invention comprises the following steps: The first step is graphite pretreatment. Add 1.0g of graphite in a 250mL conical flask, add deionized water to 100g in a 250mL conical flask, and in a 250W ultrasonic cleaning machine, perform ultrasonic treatment at room temperature for 2 hours to obtain a graphite dispersion with good dispersion properties;

[0030] The second step, prepare 1.0L of nitrifying bacteria liquid culture medium, add 1% graphite dispersion liquid that 15g first step is dispersed in the substratum, sub-pack in 4 1.0L conical flasks, and the liquid volume of every bottle is 250mL, so The graphite concentration is 0.15mg / mL. Among them, 1.0L of nitrifying bacteria liquid culture medium includes (NH 4 ) 2 SO 4 0.24g, sodium succinate 2.81g, Vickers salt solution 50mL (Vickers salt solution composition, content: K 2 HPO 4 ·3H 2 O 6.5g / L, MgSO 4 ·7H 2 O 2.5g / L, Na...

Embodiment 2

[0035] Embodiment 2: the preparation method of a kind of nitrifying bacteria biological graphite oxide of the present invention, comprises the following steps:

[0036] The first step is graphite pretreatment. Add 2.0g of graphite in a 250mL conical flask, add deionized water to 100g in the conical flask, put it in a 250W ultrasonic cleaning machine, and perform ultrasonic treatment for 4 hours at room temperature to obtain a graphite mixed dispersion with better dispersion properties;

[0037] The second step is to prepare 1.0L of nitrifying bacteria liquid culture medium, add 10g of the graphite mixed solution dispersed in the first step to the culture medium, and distribute it to four 1.0L conical flasks, the liquid volume of each bottle is 250mL, and the graphite contained The concentration is 0.2 mg / mL. Among them, 1.0L of nitrifying bacteria liquid culture medium includes (NH 4 ) 2 SO 4 0.24g, sodium succinate 2.81g, Vickers salt solution 50mL (the content is the s...

Embodiment 3

[0040] Embodiment 3: a kind of preparation method of biological graphite oxide of the present invention comprises the following steps:

[0041] The first step is graphite pretreatment. Add 0.5g of graphite in a 250mL conical flask, add deionized water to 100g in the conical flask, put it in a 250W ultrasonic cleaning machine, and perform ultrasonic treatment at room temperature for 1 hour to obtain a graphite dispersion with good dispersion properties;

[0042] The second step is to prepare 1.0L of nitrifying bacteria liquid culture medium, add 10g of the graphite mixed solution dispersed in the first step to the culture medium, and distribute it to four 1.0L conical flasks, the liquid volume of each bottle is 250mL, and the graphite contained The concentration is 0.05mg / mL. Among them, 1.0L of 9K liquid medium includes (NH 4 ) 2 SO 4 0.24g, sodium succinate 2.81g, Vickers salt solution 50mL (same as the second step of Example 1), pH 7.0, high pressure steam moist heat ...

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Abstract

The invention discloses a biologic graphite oxide obtained by nutrifying bacteria and a preparation method for the biologic graphite oxide. The preparation method comprises the following steps of: dispersing graphite in water to form a high-dispersion graphite mixed solution; adding the graphite mixed solution into a nutrifying bacteria culture medium, sterilizing, cooling and inoculating cultured nutrifying bacteria seed liquor, performing culture for many days, and introducing the dispersed graphite into oxygen-containing groups for oxidation under the action of the microbial nutrifying bacteria; after the culture is finished, removing thalli, metabolites and other ions produced by the oxidation process; and performing vacuum drying to obtain a pure biologic graphite oxide. The graphite is biologically oxidized by taking the microbial nutrifying bacteria with a low-valence state inorganic nitrogen oxidation function as strains; and compared with the conventional chemical graphite oxidation method, the method has the advantages that reaction conditions are mild and controllable, a reaction process is green and pollution-free, the prepared nutrifying bacteria biologic graphite oxide has a few shortcomings, and the like.

Description

technical field [0001] The invention belongs to the green biological controllable preparation technology of graphite oxide, in particular to a nitrifying bacteria biological graphite oxide and a preparation method thereof. Background technique [0002] At present, strong acids and strong oxidants are used in the preparation of graphene oxide by chemical oxidation, which has the disadvantages of serious environmental pollution, and the introduction of oxygen-containing groups may easily lead to lattice distortion and distortion at the atomic level of the carbon skeleton, and generate defects (Florian B ., Jani K. and Arkady V. K. Structural Defects in Graphene. ACS NANO, 2011, 5(1): 26-41), the π-π conjugated bonds were largely destroyed, and the carbon skeleton produced an obvious lattice at the atomic level Distorted and distorted, many excellent properties are lost. [0003] Nitrification is a very important link in the nitrogen (N) biogeochemical cycle, and it is also th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B31/04C01B32/23
Inventor 朱春林孙东平黄洋杨加志许威震褚云赵磊
Owner NANJING UNIV OF SCI & TECH
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