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Method for preparing procyanidolic oligomer with biological enzyme method

A technology of oligomeric proanthocyanidins and bio-enzyme method, which is applied in the field of bio-enzymatic preparation of oligomeric proanthocyanidins to achieve the effects of prolonging service life, solving easy clogging and high content

Active Publication Date: 2014-12-10
晨光生物科技集团天津有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As an excellent extraction method, bio-enzymatic extraction has the characteristics of simple and quick operation, green and pollution-free, and has been widely used in the extraction of biologically active substances. At present, there is no report on the extraction of proanthocyanidins by bio-enzymatic method.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The method for preparing oligomeric proanthocyanidins by biological enzymatic method comprises the following steps:

[0022] (1) Degreased grape seed cake was used as raw material, passed through a 90-mesh sieve, and 13 times the mass of the under-sieve was added to the purified demineralized water adjusted to pH=4 with acetic acid to obtain a mixed solution;

[0023] (2) Add enzymes to the mixture so that the mass content of the enzymes in the mixture is 0.5%, and enzymolyze at 43°C for 70 minutes; the enzymes are cathepsin and papain with a mass ratio of 1:0.5;

[0024] (3) Using a membrane vibrating filtration system, the collection can pass through a molecular weight cut-off of 5×10 3 Microfiltration membrane but can not pass through the separation liquid of nanofiltration membrane with a molecular weight of 300;

[0025] (4) The separation liquid obtained in step (3) was concentrated by an evaporator and spray-dried to obtain oligomeric proanthocyanidins with a ma...

Embodiment 2

[0027] The method for preparing oligomeric proanthocyanidins by biological enzymatic method comprises the following steps:

[0028] (1) Degreased grape seed cake was used as raw material, passed through a 90-mesh sieve, and 10 times the mass of purified demineralized water adjusted to pH=4 with phosphoric acid was added to the undersieve to obtain a mixed solution;

[0029] (2) Add enzymes to the mixture so that the mass content of the enzymes in the mixture is 0.3%, and enzymolyze at 45°C for 80 minutes; the enzyme is cathepsin;

[0030] (3) Using a membrane vibrating filtration system, the collection can pass through a molecular weight cut-off of 5×10 3 Microfiltration membrane but can not pass through the separation liquid of nanofiltration membrane with a molecular weight of 300;

[0031] (4) The separation liquid obtained in step (3) was concentrated by an evaporator and spray-dried to obtain oligomeric proanthocyanidins with a mass content of 96.1%.

Embodiment 3

[0033] The method for preparing oligomeric proanthocyanidins by biological enzymatic method comprises the following steps:

[0034] (1) Degreased grape seed cake was used as raw material, passed through a 90-mesh sieve, and the under-sieve was added 15 times the mass of purified demineralized water adjusted to pH=5 with hydrochloric acid to obtain a mixed solution;

[0035] (2) Add enzymes to the mixture so that the mass content of the enzymes in the mixture is 0.7%, and enzymatically hydrolyze at 40°C for 60 minutes; the enzymes are cathepsin and papain with a mass ratio of 1:0.8;

[0036] (3) Using a membrane vibrating filtration system, the collection can pass through a molecular weight cut-off of 5×10 3 Microfiltration membrane but can not pass through the separation liquid of nanofiltration membrane with a molecular weight of 300;

[0037] (4) The separation liquid obtained in step (3) was concentrated by an evaporator and spray-dried to obtain oligomeric proanthocyanidi...

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Abstract

The invention discloses a method for preparing a procyanidolic oligomer with a biological enzyme method. The method comprises the following steps of: sieving dregs serving as a raw material obtained by degreasing grape seeds, and adding purified and softened water of which the pH is adjusted with an acid into minus sieve to obtain a mixed liquor; adding an enzyme into mixed liquor for performing enzymolysis; collecting a separating liquid which can pass through a microfiltration membrane of which the molecular weight cutoff is 5*10<3> and cannot pass through a nanofiltration membrane of which the molecular weight is 300 by adopting a membrane vibration filtering system; and concentrating and drying the separating liquid to obtain a procyanidolic oligomer of which the mass content is over 95 percent. The method is easy and convenient to operate, is environment-friendly, and is easy to industrialize. Due to the adoption of the membrane vibration filtering system for preparing the procyanidolic oligomer, the defects of easiness in blocking and difficulty in cleaning existing in a static membrane filtering method are overcome, and the service life of a membrane is prolonged. The procyanidolic oligomer extracted by using the method has high content.

Description

technical field [0001] The invention relates to a method for preparing oligomeric proanthocyanidins by a biological enzyme method. Background technique [0002] The oligomeric proanthocyanidins in grape seeds are natural antioxidant functional factors that eliminate active oxygen free radicals, and can be used in health food, medicine, cosmetics and other fields. Oligomeric proanthocyanidins have strong anti-oxidation, free radical scavenging ability and special improvement of human microcirculation, and are famous for their high efficiency, low toxicity and high bioavailability. According to data reports, its antioxidant capacity in vivo is 50 times that of VE and 20 times that of VC. In the past 10 years, it has been widely used in the North American health food industry and cosmetic industry. [0003] Commonly used proanthocyanidin extraction methods include Soxhlet extraction, solvent extraction, etc. Soxhlet extraction has a long extraction time and is difficult to e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62
Inventor 张卫军王如
Owner 晨光生物科技集团天津有限公司
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