Method for preparing bee pollen polysaccharide through combining enzymolytic wall-breaking with hot-water ultrasonic extracting

A technology of ultrasonic extraction and bee pollen, which is applied in the chemical industry and can solve problems such as cumbersome operation steps

Inactive Publication Date: 2014-04-02
TONGLU XINGYUAN HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The patent number is 2011103908202 and the name is the preparation method of the active ingredient polysaccharide in the schisandra medicinal residue, and the patent number is 2011103653237. In the two inventions, the name is a separation and purification method of the dendrobium polysaccharide, both adopt the Sevage deproteinization method, and the disadvantage is that both require Repeat the deproteinization for about 5 times with the Sevage deproteinization method to obtain polysaccharides with high purity, and the operation steps are cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: This embodiment consists of the following steps:

[0040] 1. Firstly, the bee pollen is dried, impurity-removed and sieved, and then sieved mechanically to form bee pollen powder;

[0041] 2. Add 0.5% by mass of cellulase to the bee flower powder, and then add 9 times the mass of water to form a mixture of cellulase and bee flower powder;

[0042] 3. Heat the cellulase bee pollen powder mixture, the temperature is controlled at 42 degrees, and the enzymatic decomposition reaction time is 90 minutes; the bee pollen aqueous solution that is pasty after the wall is broken;

[0043] 4. Heat the paste-like bee pollen aqueous solution after breaking the wall, temperature control 82 degrees, ultrasonic power 400W, extraction 2 times, 2.5 hours each time, the polysaccharides in the paste-like bee pollen aqueous solution are completely decomposed in the aqueous solution ;

[0044] 5. Add organic solvent pure edible ethanol (mixed mass ratio of bee pollen aqueous solution an...

Embodiment 2

[0051] Embodiment 2: This embodiment consists of the following steps:

[0052] 1. Firstly, the bee pollen is dried, impurity-removed and sieved, and then sieved mechanically to form bee pollen powder;

[0053] 2. Add 0.1% mass of cellulase to the bee flower powder, and then add 6 times the mass of water to form a cellulase bee flower powder mixture;

[0054] 3. Heat the cellulase bee pollen powder mixture, the temperature is controlled at 42 degrees, and the enzymatic decomposition reaction time is 90 minutes; the bee pollen aqueous solution that is pasty after the wall is broken;

[0055] 4. Heat the paste-like bee pollen aqueous solution after breaking the wall, temperature control 82 degrees, ultrasonic power 400W, extraction 2 times, 2.5 hours each time, the polysaccharides in the paste-like bee pollen aqueous solution are completely decomposed in the aqueous solution ;

[0056] 5. Add 30% mass organic solvent pure edible ethanol to the leached bee pollen aqueous solution to dehydr...

Embodiment 3

[0063] Embodiment 3: This embodiment consists of the following steps:

[0064] 1. Firstly, the bee pollen is dried, impurity-removed and sieved, and then sieved mechanically to form bee pollen powder;

[0065] 2. Add 1% by mass of cellulase to the bee flower powder, then add 8 times the mass of water to form a cellulase bee flower powder mixture;

[0066] 3. Heat the cellulase bee pollen powder mixture, the temperature is controlled at 42 degrees, and the enzymatic decomposition reaction time is 90 minutes; the bee pollen aqueous solution that is pasty after the wall is broken;

[0067] 4. Heat the paste-like bee pollen aqueous solution after breaking the wall, temperature control 82 degrees, ultrasonic power 400W, extraction 2 times, 2.5 hours each time, the polysaccharides in the paste-like bee pollen aqueous solution are completely decomposed in the aqueous solution ;

[0068] 5. Add 40% mass organic solvent pure edible ethanol to the extracted bee pollen aqueous solution to dehydra...

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PUM

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Abstract

The invention discloses a method for preparing bee pollen polysaccharide through combining enzymolytic wall-breaking and hot-water ultrasonic extracting. The method comprises the following steps: drying, purifying, grinding and screening bee pollen; carrying out enzymolytic wall-breaking; carrying out heating and ultrasonic extracting; dehydrating; deproteinizing with perchloric acid; carrying out (diethylamino) ethyl column chromatography and elution; separating acid polysaccharide precipitation and neutral polysaccharide precipitation with cetyl trimethyl ammonium bromide (CTAB); and dissolving the polysaccharide precipitations, then dehydrating, vacuum-freezing, and drying to obtain the high-purity impurity-free bee pollen polysaccharide. By the method, the polysaccharide extraction ratio is improved by 66.03%, and the polysaccharide yield reaches 18.54mg / g which is improved by 2.02 times compared with that of the extraction method of the prior art; and the obtained bee pollen polysaccharide liquid is negative relatively to the ninhydrin reaction, no protein residual is left, and the obtained bee pollen polysaccharide has no impurities and high purity.

Description

Technical field [0001] The invention is a method for enzymatic hydrolysis of bee pollen polysaccharides combined with hot water ultrasonic extraction method, belonging to the technical field of chemical industry. Background technique [0002] Bee pollen contains a certain amount of protein, free amino acids, multiple active enzymes, multiple vitamins, nucleic acids, minerals, carotene, auxin, phospholipids, sugars and other nutrients. It is a pure natural high-grade non-toxic and no side effects Green food is known as a concentrated body of nutrients. Modern studies have proved that bee pollen can be promoted into high body T lymphocytes (T lymphocytes are derived from embryonic liver or bone marrow original T cells, differentiate and develop in the thymus microenvironment, and settle in the lymphatic tissues in the peripheral blood after maturation; T cells can be Mediates cellular immune response and assists the body to produce humoral immunity against T cell-dependent antigen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C08B37/06C08B30/04
Inventor 袁文明
Owner TONGLU XINGYUAN HEALTH PROD
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