Method for preparing bee pollen polysaccharide through combining enzymolytic wall-breaking with hot-water ultrasonic extracting

Abstract

The invention discloses a method for preparing bee pollen polysaccharide through combining enzymolytic wall-breaking and hot-water ultrasonic extracting. The method comprises the following steps: drying, purifying, grinding and screening bee pollen; carrying out enzymolytic wall-breaking; carrying out heating and ultrasonic extracting; dehydrating; deproteinizing with perchloric acid; carrying out (diethylamino) ethyl column chromatography and elution; separating acid polysaccharide precipitation and neutral polysaccharide precipitation with cetyl trimethyl ammonium bromide (CTAB); and dissolving the polysaccharide precipitations, then dehydrating, vacuum-freezing, and drying to obtain the high-purity impurity-free bee pollen polysaccharide. By the method, the polysaccharide extraction ratio is improved by 66.03%, and the polysaccharide yield reaches 18.54mg / g which is improved by 2.02 times compared with that of the extraction method of the prior art; and the obtained bee pollen polysaccharide liquid is negative relatively to the ninhydrin reaction, no protein residual is left, and the obtained bee pollen polysaccharide has no impurities and high purity.

Description

Technical field

[0001] The invention is a method for enzymatic hydrolysis of bee pollen polysaccharides combined with hot water ultrasonic extraction method, belonging to the technical field of chemical industry. Background technique

[0002] Bee pollen contains a certain amount of protein, free amino acids, multiple active enzymes, multiple vitamins, nucleic acids, minerals, carotene, auxin, phospholipids, sugars and other nutrients. It is a pure natural high-grade non-toxic and no side effects Green food is known as a concentrated body of nutrients. Modern studies have proved that bee pollen can be promoted into high body T lymphocytes (T lymphocytes are derived from embryonic liver or bone marrow original T cells, differentiate and develop in the thymus microenvironment, and settle in the lymphatic tissues in the peripheral blood after maturation; T cells can be Mediates cellular immune response and assists the body to produce humoral immunity against T cell-dependent antigen...