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Preparation method of sodium hyaluronate gel

The technology of sodium hyaluronate and gel is applied in the fields of biomedicine, medical cosmetology, and cosmetic surgery, which can solve the problems of increased difficulty in use, increased extrusion pressure, uneven stress surface, etc., and achieves excellent biocompatibility, The effect of strengthening the mechanical strength and delaying the degradation rate

Inactive Publication Date: 2012-10-31
颜先琴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] When preparing sodium hyaluronate gel, the above-mentioned patented methods all adopt a single cross-linking method, so that the final extrusion pressure of the sodium hyaluronate gel product will increase, increase the difficulty of use, and form unevenness in the dialysis step. The stress surface, the residual amount of crosslinking agent will be different on different stress surfaces

Method used

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  • Preparation method of sodium hyaluronate gel
  • Preparation method of sodium hyaluronate gel

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Step 1), in the alkaline solution with a pH value of 10, add 0.01% (weight) of cross-linking agent after shaking and dissolving, then add 10% (weight) of high-purity sodium hyaluronate raw material, at room temperature 25 ° C, In an environment where the number of 0.5um particles is less than 3500, the temperature is controlled at 27°C, 3800 rpm high-speed rotation and stirring for 30 minutes, and then heated at a constant temperature of 50°C to obtain a sodium hyaluronate molded product;

[0031] Step 2), at room temperature 25°C, in an environment where the number of 0.5um particles is controlled to be less than 3500, cut the molded object into 1cm 3 The lumps are put into the solution of normal saline and phosphate ratio, the mass ratio of the lumps to the solution is controlled at 1:1000, so that the contact area between the lumps and the solution is the same, and the contact time is controlled at 20 hours. Sodium hyaluronate gel;

[0032] Wherein, the NaCl of desc...

Embodiment 2

[0038] Step 1) The pH value of the alkaline solution is 11, the high-purity sodium hyaluronate raw material is 15% (weight), the temperature is controlled at 28°C, and the high-speed rotation is stirred at 4000 rpm, and the others are the same as in Example 1;

[0039] In step 2), the molding is cut into 1cm 3 The lump, contact time is controlled at 21 hours, and other is with embodiment 1;

[0040] Step 3) 50 mesh stainless steel screens are used for net screening, and others are with embodiment 1;

[0041] Step 4) is the same as embodiment 1;

[0042] Step 5) stirring speed is 3800 rev / min, other is with embodiment 1;

[0043] Step 6) is the same as in Example 1.

Embodiment 3

[0045] Step 1) The pH value of the alkaline solution is 12, the high-purity sodium hyaluronate raw material is 20% (by weight), the temperature is controlled at 29 ° C, and the high-speed rotation is stirred at 4200 rpm, and the others are the same as in Example 1;

[0046] In step 2), the molding is cut into 2cm 3 The lump, contact time is controlled at 22 hours, and other is with embodiment 1;

[0047] Step 3) 80 mesh stainless steel screens are used for net screening, and others are with embodiment 1;

[0048] Step 4) is the same as embodiment 1;

[0049] Step 5) stirring speed is 3900 rev / min, other is with embodiment 1;

[0050] Step 6) is the same as in Example 1.

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Abstract

The invention discloses a preparation method of a sodium hyaluronate gel. The method comprises the steps that: (1) a cross linking agent is dissolved in an alkaline solution; a sodium hyaluronate raw material is added and dissolved; and the mixture is subjected to constant-temperature heating, such that a molded material is prepared; (2) the molded material is cut into blocks; the blocks are placed in a solution with physiological saline and phosphate with a certain ratio; and after 20-22h, a gel is prepared; (3) the obtained gel is subjected to particle grading by using a sieve, such that sodium hyaluronate gel particles are obtained; (4) a sodium hyaluronate raw material is added and dissolved in injection water, such that sodium hyaluronate hydrogel is obtained; (5) the sodium hyaluronate obtained in the steps (3) and (4) are mixed and cross-linked, such that a sodium hyaluronate gel is obtained; and (6) the obtained gel is sterilized under a temperature below 121 DEG C, such that the sodium hyaluronate gel is obtained. With the method, the prepared sodium hyaluronate gel is advantaged in relatively high thermal stability, relatively high degradation resistance, and good biocompatibility. The gel can swell in water, but cannot be dissolved. The gel has better rheological characteristic, and substantially improved mechanical strength.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of a sterile biomaterial sodium hyaluronate gel used for subcutaneous filling, which can be applied to the fields of medical cosmetology and plastic surgery such as wrinkle removal, lip augmentation and filling. Background technique [0002] At present, there are many preparation methods for the aseptic biological material sodium hyaluronate gel used for subcutaneous filling, such as a preparation process of sodium hyaluronate gel particles in Chinese invention patent CN101264348; CN101502677A a cross-linked transparent Sodium hyaluronate gel and preparation method thereof CN101244290A A cross-linked hyaluronic acid microgel for tissue filling, a preparation method thereof, and the like. [0003] When preparing sodium hyaluronate gel, the above-mentioned patented methods all adopt a single cross-linking method, so that the final extrusion pres...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08L5/08C08K5/1515A61L27/20A61L31/04
Inventor 颜先琴
Owner 颜先琴
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