Amino acid fertilizer and preparation method thereof
An amino acid and fertilizer technology, applied in the field of fertilizers, can solve problems affecting the normal life of surrounding residents, waste of resources, etc., and achieve strong attenuation effect, low production cost, and high base replacement effect
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Embodiment 1
[0025] Embodiment 1, bacillus subtilis ( Bacillus subtilis ) separation of RB
[0026] Mix 10g of beneficial microorganisms isolated and screened from nature evenly with the nutrient base, which contains 5-10g of cornstarch, 5-10g of brown sugar and 10-20g of wheat bran, pH 6.0-7.0, humidity 30-35%, and then Add 0.005-0.05 g of hydroxylamine or ethyl imine; at a temperature of 25-35° C. with a temperature difference of 8-10° C., cultivate for 2-3 days, and use an inoculation loop to screen out Bacillus subtilis RB. The nutrient base may also include 3-5 g of fish meal, 3-5 g of vegetable cake or 35-50 g of polished rice bran.
Embodiment 2
[0027] Embodiment 2, the cultivation of Bacillus subtilis RB
[0028] Bacillus subtilis RB medium: sucrose 50g, (NH 4 ) 2 SO 4 6g, K 2 SO 4 2.5g, CaSO 4 2.5g, agar powder 15g and distilled water 1000mL.
[0029] The culture conditions are: the temperature is 30° C., and the pH is 5.8.
Embodiment 3
[0030] Embodiment 3, the preparation of amino acid fertilizer
[0031] The enzyme bacteria of the present invention can be obtained by mixing Bacillus subtilis RB with the commercially available enzyme bacteria in a weight ratio of 1-2:5.
[0032] The raw material of amino acid fertilizer consists of the following components in parts by weight:
[0033] Formula 1: 1 part of enzyme bacteria, 20 parts of poultry manure and 50 parts of Artemisia annua residue.
[0034] Formula 2: 2 parts of enzyme bacteria, 30 parts of poultry manure and 70 parts of Artemisia annua dregs.
[0035] Formula 3: 1.2 parts of enzyme bacteria, 25 parts of poultry manure and 60 parts of Artemisia annua dregs.
[0036] The preparation method of amino acid fertilizer, concrete steps are as follows:
[0037] Preparation method 1: Enzyme bacteria, poultry manure and Artemisia annua slag are mixed according to the above-mentioned ratio, and after stirring, open aerobic solid-state fermentation at a temp...
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