Method for establishing adventitious root cultivation system of Psammosilene tuniceoides W. C. Wu et C. Y. Wu and expanding cultivation method of Psammosilene tuniceoides W. C. Wu et C. Y. Wu

A technology for expanding the cultivation and cultivation system, which is applied in the establishment and expansion of the cultivation system of the adventitious root of Aurantia chinensis, and can solve the problems of retention, growth stability, cultivation conditions and various adjustment parameters, such as the contradiction between cell growth and the accumulation of secondary metabolites , to achieve stable output and quality, rapid and convenient scale production, and reduce production costs

Active Publication Date: 2013-11-27
成都市三禾田生物技术有限公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0006] But so far, the plant cell culture of Jintiezi still stays at the level of in vitro culture and rapid propagation research. There is no information about the use of plant cell culture technology to cultivate Jintiezi tissue, the intracellular synthesis pathway of Jintiezi secondary metabolite saponin, and through comprehensive regulation A report on improving the plant cell biomass of Jintiesuo by technology, and then increasing the content of secondary metabolites such as total saponins of Jintiesuo
The main problem is that there is insufficient research on the growth stability, culture conditions and various regulatory parameters, cell growth and the accumulation of secondary metabolites in the plant cell culture of Jintie Suo.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] (1) Select Saccharomyces auricosa as explants, and carry out disinfection treatment: soak in running water for 25 minutes with Tween 20, rinse with sterile water for 3 times; then use 0.1% HgCl 2 Treat for 8 minutes, rinse with sterile water for 3 times; then treat with 75% ethanol for 8 seconds, rinse with sterile water for 3 times, and finally dry the water with sterile filter paper;

[0060] (2) Callus induction: inoculate the explants in step (1) in callus induction medium: MS medium + 2,4-D 0.5 mg / L + NAA 0.5 mg / L + KT 0.1mg / L+cysteine ​​1.0mg / L, pH 6.0, cultivate in dark at 25±1°C. The leaves were inoculated in the induction medium for about 10 days and began to grow milky white callus; about 20 days, the callus gradually covered the explant, and at 25 days, the callus with loose texture and good growth was selected for subculture;

[0061] (3) Induced callus subculture: Take 0.5 g of callus obtained in step (2) and inoculate it into MS medium + sucrose 40g / L + ...

Embodiment 2

[0067] (1) Select the 1.0-1.5cm length of the golden iron lock stem section as the explant, and carry out disinfection treatment: after washing with running water, soak it in Tween 20 for 25 minutes, rinse it with sterile water for 3 times; then use 0.1% HgCl 2 Treat for 8 minutes, rinse with sterile water for 3 times; then treat with 75% ethanol for 8 seconds, rinse with sterile water for 3 times, and finally dry the water with sterile filter paper;

[0068] (2) Callus induction: inoculate the explants in step (1) in callus induction medium: MS medium + 2,4-D 0.5 mg / L + NAA 0.5 mg / L + KT 0.1mg / L+ cysteine ​​2.0mg / L, pH 6.0, cultivate in dark at 25±1°C. The leaves were inoculated in the induction medium for about 10 days and began to grow milky white callus; about 20 days, the callus gradually covered the explant, and at 25 days, the callus with loose texture and good growth was selected for subculture;

[0069] (3) Induced callus subculture: Take 0.5 g of callus obtained in...

Embodiment 3

[0076] (1) Select the 2.0-3.0cm length of the golden iron lock stem section as the explant, and carry out disinfection treatment: soak in Tween 20 for 25 minutes after washing with running water, and rinse with sterile water for 3 times; then use 0.1% HgCl 2 Treat for 8 minutes, rinse with sterile water for 3 times; then treat with 75% ethanol for 8 seconds, rinse with sterile water for 3 times, and finally dry the water with sterile filter paper;

[0077] (2) Callus induction: inoculate the explants in step (1) in callus induction medium: MS medium + 2,4-D 0.5 mg / L + NAA 0.5 mg / L + KT 0.1mg / L, pH 6.0, cultured at 25±1°C under natural light. The leaves were inoculated in the induction medium for about 10 days and began to grow milky white callus; about 20 days, the callus gradually covered the explant, and at 25 days, the callus with loose texture and good growth was selected for subculture;

[0078] (3) Induced callus subculture: Take 0.5 g of callus obtained in step (2) an...

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PUM

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Abstract

According to the invention, young leaves or stems of plants of Psammosilene tuniceoides W. C. Wu et C. Y. Wu are taken as an explant to successfully induce callus of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu, and a callus culture system of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu under the conditions of light and dark cultivation is established to induce the differentiation of the callus to produce adventitious roots, thus establishing an adventitious root cultivation system of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu. Moreover, the content of total saponins of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu is determined, and the conditions and parameters of plant cell culture are further optimized, thus establishing a high-yield cell culture system of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu. Therefore, the plant cells of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu are cultured in a large scale to produce the adventitious roots which substitute for the original plant of the Psammosilene tuniceoides W. C. Wu et C. Y. Wu to be used as medicine.

Description

technical field [0001] The invention relates to a method for inducing and cultivating adventitious root systems using plant tissue culture technology, in particular to a method for inducing and cultivating adventitious root systems in the form of gold-iron locks and enlarging bioreactors. Background technique [0002] Psammosilene tuniceoides (Psammosilene tuniceoides W. C. Wu et C. Y. Wu) is a plant of the genus Psammosilene tuniceoides in Caryophyllaceae, and it is a unique medicinal plant in Southwest my country. The root of Jintiesuo is mainly used as medicine, and it is often used clinically to treat rheumatoid arthritis and other diseases. The main chemical components of Jintiesuo are triterpenes, triterpene saponins, cyclic peptides and lactams. Pharmacological tests have shown that total saponins of Jintiesun have obvious analgesic and anti-inflammatory effects. The traditional method of obtaining Chinese herbal medicine is at the cost of collecting and consuming a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 柴素真洪汉君张宗申熊小灿冯敏
Owner 成都市三禾田生物技术有限公司
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