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Method for removing heavy metal in marine animal protein enzymed liquid

A proteolysis solution and marine animal technology, applied in the field of removing heavy metals in marine animal proteolysis solution, can solve problems such as not suitable for large-scale use, complicated operation process, loss of nutrients, etc., achieve low investment and operation costs, and simple process operation , the effect of small loss of nutrients

Inactive Publication Date: 2012-11-21
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it has disadvantages such as high energy consumption and environmental protection.
The adsorption method uses the adsorption properties of the adsorption material to adsorb heavy metals, but because the proteolysis solution is a very complex system, mainly including functional components such as proteins, active peptides and amino acids, the adsorbent is not selective in the adsorption process. Causes loss of nutrients, and is not suitable for large-scale use due to complicated operating procedures
The complexation method utilizes the chelating ability of complexing reagents for heavy metals for the removal of heavy metals. Although it has its advantages in selectivity and removal rate, it is difficult to separate during the removal process. After the complexing agent is added, it only changes The existing forms of heavy metals can reduce their toxicity to a certain extent, but still have not really solved the problem of excessive heavy metals

Method used

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  • Method for removing heavy metal in marine animal protein enzymed liquid
  • Method for removing heavy metal in marine animal protein enzymed liquid
  • Method for removing heavy metal in marine animal protein enzymed liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0027] After shelling the oysters and taking the meat, add 2 times the volume of deionized water to homogenize for 5 minutes, sonicate for 10 minutes, then add neutral protease, the amount of enzyme added is 3000U / g, the enzymatic hydrolysis condition is pH=7, the enzymatic hydrolysis time is 4 hours, and the enzymatic hydrolysis The temperature is 50°C. After the reaction, inactivate the enzyme at 100°C for 10 minutes, centrifuge at high speed for 15 minutes, and add 3.0‰ (W 柠檬酸 / W 蛋白酶解液 ) was mixed with citric acid, the temperature was adjusted at 60°C, the reaction time was 20min, and the supernatant was obtained by centrifugation; the supernatant was adjusted to pass through D401 at a reaction temperature of 50°C, a pH of 6, and a column flow rate of 5 BV / h. Porous chelating resin column is used to remove heavy metals, and the enzymatic hydrolysis solution for removing harmful substances is obtained. The heavy metal content of the enzymatic solution before and after remov...

Embodiment 2

[0034] Remove the shell from Pinctada martensii and add 2 times the volume of deionized water to homogenize for 5 minutes, sonicate for 10 minutes, then add flavor protease, the amount of enzyme added is 7200U / g, the enzymatic hydrolysis condition is pH=7, and the enzymatic hydrolysis time is 5h , the enzymatic hydrolysis temperature is 60°C, after the reaction, the enzyme is extinguished at 100°C for 10 minutes, centrifuged at high speed for 15 minutes, and after filtering, add 3.0‰ (W 柠檬酸 / W 蛋白酶解液 ) was mixed with citric acid, the temperature was adjusted at 60°C, the reaction time was 20min, and the supernatant was obtained by centrifugation; the supernatant was adjusted to pass through D401 at a reaction temperature of 50°C, a pH of 5.5, and a column flow rate of 5 BV / h. Porous chelating resin column is used to remove heavy metals, and the enzymatic hydrolysis solution for removing harmful substances is obtained. The heavy metal content of the enzymatic solution before an...

Embodiment 3

[0041] Remove the internal organs of the cod and take the meat, add 3 times deionized water to homogenate for 5 minutes, sonicate for 10 minutes, first add neutral protease for enzymatic hydrolysis, the amount of enzyme added is 3000U / g, the enzymatic hydrolysis condition is pH 7, and the enzymatic hydrolysis time is 3 hours, and then the Adjust the pH to 9, add alkaline protease for enzymolysis, the amount of enzyme added is 800U / g, the enzymolysis time is 3h, and the enzymolysis temperature is kept at 55°C. After the reaction is completed, the enzyme is extinguished at 100°C for 10min, high-speed centrifugation for 15min, and filtered in the proteolysis solution Add 1.0‰(W 柠檬酸 / W 蛋白酶解液 ) mixed with citric acid, the temperature was adjusted at 50°C, the reaction time was 15min, and the supernatant was centrifuged; the supernatant was adjusted to pass through the ion exchange resin at a reaction temperature of 50°C, a pH of 6, and a column flow rate of 15BV / h. The column is u...

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Abstract

The invention relates to a method for removing heavy metal in a marine animal protein enzymed liquid, which belongs to the biology technical field, and more concretely relates to the method for removing the heavy metal in the marine animal protein enzymed liquid. The method for removing heavy metal in the marine animal protein enzymed liquid has the advantages of easy operation, economical and practical properties and high removal rate. By using a method for combining the organic acid complexing and the resin adsorption, the heavy metal can be removed from the marine animal protein enzymed liquid.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and more specifically relates to a method for removing heavy metals from marine animal protein enzymatic hydrolyzate. Background technique: [0002] The marine protein hydrolyzate contains a large number of bioactive peptides, which have unique physiological activities and have special effects in immune regulation, tumor suppression, enzyme inhibition, antibacterial, antiviral, antioxidation, antiradiation, etc. In recent years, as its function has been gradually recognized by people, it has been widely used in food, medical care and cosmetics industries. However, in the preparation process, the quality and safety of active peptide base materials is an important bottleneck restricting the rapid development of this industry group, and the problem of excessive heavy metals is particularly serious. In addition to proteolysis solution, the quality of other marine products such as surimi and seafood sau...

Claims

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Application Information

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IPC IPC(8): C07K2/00C07K1/36C07K1/22C07K1/14C12P21/06
Inventor 秦松杨小满孙恢礼潘剑宇郭占勇焦绪栋
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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