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Method for preparing chang date antineoplastic active polysaccharides by separation and purification

An anti-tumor activity, separation and purification technology is applied in the field of separation, purification and preparation of long jujube anti-tumor active polysaccharide, which can solve the problem of lack of long jujube research, and achieves overcoming unclear anti-tumor activity, improving yield, and preserving long jujube polysaccharide. The effect of biological activity

Inactive Publication Date: 2015-06-17
NINGXIA UNIVERSITY
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

These patent reports have greatly enriched the extraction and purification methods of jujube polysaccharides, but did not conduct research on the unique dominant variety of jujube in Ningxia

Method used

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Experimental program
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Embodiment Construction

[0023] Select the dried Ningxia Lingwu Changzao, dry the raw materials at 50-60°C, let them cool, then crush the raw materials with a high-speed pulverizer, pass the pulverized raw materials through an 80-mesh sieve, and put the slag on the upper part of the sieve into the pulverizer again Re-shred in. Extract a certain quality of jujube powder sample in a water bath at 80-100°C for about 2 hours at a solid-to-liquid ratio of 1:4. After centrifugal filtration, the filter residue was leached again in a water bath, and this was repeated 3 times.

[0024] The obtained clear liquid is repeatedly decolorized through the activated carbon powder sand core column until the color of the liquid does not change. Add anhydrous ethanol to precipitate and freeze-dry for use.

[0025] The DEAE-52 cellulose is washed with 0.5-1mol / L sodium hydroxide solution, ultrapure water, and 0.5-1mol / L hydrochloric acid solution for alkali washing-acid washing-alkali washing-ultrapure water washing res...

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Abstract

The invention relates to a preparation method of chang date antineoplastic active polysaccharides by separation and purification, characterized by using Ningxia Lingwu chang date as a raw material. The separation and purification process is characterized by 1) preparing a DEAE-52 cellulose packed column: conducting alkali washing, pickling, alkali washing, ultrapure water washing respectively by using 0.5-1mol / L sodium hydroxide solution, ultrapure water, and 0.5-1mol / L hydrochloric acid solution, and filling in a packed column; 2) letting an extract processed by decoloring and dialyzing pass through the cellulose packed column, conducting gradient elution respectively by using double distilled water and 0.1-0.5mol / L NaCl solution, determining the absorbance to no effusion of sugar by a spectro photometer, and collecting ingredients according to the peak condition, wherein the flow velocity of each tube is 3-4min, and 5ml is collected in each tube; 3) dialyzing with a dialysis bag to desalt, condensing, then conducting alcohol precipitation, centrifugation and freeze drying; and 4) purifying by a DEAE-52 gel column to obtain classified and purified chang date single-component polysaccharides(LJU-0, LJU-1, LJU-2, LJU-3, LJU-4, LJU-5).

Description

technical field [0001] The invention relates to a method for preparing polysaccharides, in particular to a method for separating, purifying and preparing polysaccharides with anti-tumor activity from jujube. Background technique [0002] Polysaccharides are another biologically active macromolecule in addition to proteins and nucleic acids that exist in natural products. As information molecules, they determine the phenotype and antigenic structure of some cells and molecules, control cell division or differentiation, and regulate cell growth and aging. Is a non-cytotoxic immune booster. A series of physiological and pathological processes in animals are directly related to the participation of sugar chains in the recognition of cells. There are 500,000 glycoprotein molecules on the surface of a red blood cell, and a slight change in the monosaccharide composition and sugar shape of the IgG sugar chain in the human body will cause a certain disease, such as when the galacto...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P35/00
Inventor 李亚蕾罗瑞明杨波李海峰李文霞章中柳杨刘晓连卢君逸
Owner NINGXIA UNIVERSITY