Method for detecting glucose through using gold nano-particles and silver mirror reaction
A gold nanoparticle and silver mirror reaction technology, applied in the field of glucose detection, can solve problems such as difficulty in storage, and achieve the effects of simple operation, less sample consumption and high sensitivity
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Embodiment 1
[0032] Gold nanoparticles stabilized with citrate on the surface are obtained by the existing method of sodium citrate reduction of chloroauric acid, the specific steps are as follows: heat 0.08M, 125mL chloroauric acid aqueous solution to make it boil, quickly add 38.8mM, 10mL citric acid trisodium solution, the color of the solution will change from dark yellow to deep red. After the color changes, continue to heat and reflux for 30 minutes, and then cool at room temperature to obtain nanoparticles with a diameter of about 12-14 nm and a surface stabilized with citrate salt. This preparation method is a relatively common and mature method.
[0033] The outer layer of the surface of the gold nanoparticle is the carboxyl group of citrate, the aqueous solution thereof is red, and the characteristic ultraviolet peak is around 520nm.
Embodiment 2
[0035] 1) Use distilled water to prepare a series of known concentrations of glucose aqueous standard (A 1 , A 2 ......A n ), the concentrations are 0mM, 0.001mM, 0.01mM, 0.04mM, 0.08mM, 0.1mM, 0.2mM, 0.4mM, 0.8mM, 1.0mM, 2.0mM, 4.0mM, 10mM.
[0036] 2) Using gold nanoparticles stabilized with citrate on the surface and silver ammonia solution as detection reagents, add 20 μL of gold nanoparticles stabilized with citrate on the surface at a concentration of 10 nM to 145 μL of distilled water, and then add 20 μL of different concentrations of glucose solution (A 1 , A 2 ......A n ), and finally add 15 μL of silver ammonia solution with a concentration of 24mM to form a mixed solution (M 1 ,M 2 ...... M n ), room temperature reaction, 15min.
[0037] 3) After the reaction, the color of the solution changed from red to yellow. With the increase of the concentration of glucose added, the ultraviolet absorption of the mixed solution at 410nm gradually increased and finally ...
Embodiment 3
[0041] The time required for the detection reaction was studied, and the specific steps include:
[0042] 1) Using citrate-stabilized gold nanoparticles on the surface and silver ammonia solution as detection reagents, add 20 μL of citrate-stabilized gold nanoparticles with a concentration of 10 nM into 145 μL of distilled water, and then add 20 μL of 1mM glucose aqueous solution, and finally add 15μL silver ammonia solution with a concentration of 24mM to form a mixed solution, and react at room temperature.
[0043] 2) Measure the ultraviolet absorption of the mixed solution at 410nm at 1min, 3min, 10min, 15min, 20min, 30min, 45min, 60min and 90min, and draw it as Figure 4 .
[0044] In this example, the diameter of gold nanoparticles stabilized with citrate on the surface is 13 nm.
[0045] Such as Figure 4 As shown, after the detection reaction at room temperature is carried out for 15 minutes, the ultraviolet absorption of the mixed solution at 410 nm reaches the max...
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Abstract
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