High water retention microbe medium and preparation method

A microbial culture medium and water retention technology, applied in the direction of fungi, bacteria, etc., can solve the problems of easy crystallization or cracking, prolonging the culture time of microorganisms, and dehydration of the medium

Active Publication Date: 2012-11-28
浙江天科高新技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Agar is the most widely used coagulant, but there are still problems: 1) The moisture in the gel is easy to volatilize; 2) The condensed water accumulates on the wall of the petri dish and easily causes contamination between colonies; 3) Freezing is prone to crystallization or cracking, which is not conducive to Strain subculture and long-term preservation; 4) Overexploitation leads to high prices
Due to the need to use a large amount of organic solvents in the reverse phase suspension polymerization method, the main disadvantages are: the complexity of the synthesis process, the increase in cost, and the pollution to the environment
During the implementation of the natural sedimentation method of the petri dish, the petri dish needs to be exposed to the air, but because the moisture in the petri dish will gradually lose with the prolongation of the opening time, it will affect the growth of microorganisms settled on the medium. Reduce the number of microorganisms that can grow, and ultimately affect the accuracy of experimental data
During the implementation of the automatic collection method using the sampler, due to the effect of the air flow, the water on the medium will also be taken away, and eventually the water in the culture dish will be gradually lost, which will eventually affect the accuracy of the experimental data
[0007] In order to solve the problem of medium water loss during the collection and cultivation of microorganisms and prolong the cultivation time of microorganisms, we have developed a super absorbent resin for microbial culture medium

Method used

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  • High water retention microbe medium and preparation method
  • High water retention microbe medium and preparation method
  • High water retention microbe medium and preparation method

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preparation example Construction

[0147] The steps of the preparation method of the microbial culture medium with high water retention are as follows:

[0148] 1) Preparation of super absorbent resin

[0149] (1) First dissolve the alkali with a mass ratio of 1:10~1:1 to the monomer in water in an ice-water bath (or do not add alkali when the main monomer is a non-acidic monomer); then slowly drop into the monomer It is configured into a monomer solution with a concentration of 5-90% (this monomer can be one kind, or it can be a co-monomer composed of a hydrophobic monomer with a mass ratio of 0.01%-1% of the main monomer) , keep the system at a certain temperature, do not make the local temperature too high; then add a cross-linking agent with a mass ratio of 1:10000~1:10 and an initiator with a mass ratio of 1:10000~1:10 to dissolve it In solution;

[0150] (2) Put the reaction vessel in a water bath at 70-90°C and heat it for 0.5-5 minutes while stirring magnetically, or put the reaction vessel in a micro...

Embodiment 1

[0169] 1) Preparation of super absorbent resin

[0170] ①First, dissolve sodium hydroxide with a mass ratio of 3:10 to acrylic acid in water in an ice-water bath; then slowly drop in acrylic acid to make it into an acrylic acid solution with a concentration of 30%, keep the system at a certain temperature, do not use The local temperature is too high; then add N, N`-methylenebisacrylamide with a mass ratio of 1:1000 to acrylic acid and potassium persulfate at 1:1000, and dissolve them in the solution;

[0171] ② Put the reaction vessel into a water bath at 70°C and heat for 5 minutes while magnetically stirring. Or put the reaction vessel into the microwave oven for 6 minutes and let it rotate to heat evenly. Take it out immediately after the reaction is over, then place it at room temperature, and slowly lower the system temperature to room temperature;

[0172] ③ Put it in a 45°C oven for 6 hours;

[0173] ④ Raise the temperature to 85°C, continue the reaction for 30 minu...

Embodiment 2

[0179] 1) Preparation of super absorbent resin

[0180]①First, dissolve sodium hydroxide with a mass ratio of 2:5 to methacrylic acid in water in an ice-water bath; then slowly drop methacrylic acid to make it into a methacrylic acid solution with a concentration of 40%, keeping the system at a certain In the temperature, do not make the local temperature too high; then add polyethylene glycol bismaleate and potassium persulfate of 1:500 with the mass ratio of methacrylic acid, and dissolve it in the solution;

[0181] ② Put the reaction vessel into a water bath at 75°C and heat for 4 minutes while magnetically stirring. Or put the reaction vessel into the microwave oven for 5 minutes and let it rotate to heat evenly. Take it out immediately after the reaction is over, then place it at room temperature, and slowly lower the system temperature to room temperature;

[0182] ③ Put it in an oven at 50°C for 5 hours;

[0183] ④ Raise the temperature to 87°C, continue the reactio...

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Abstract

The invention discloses a microbe medium possessing high water retention and a preparation method, the microbe medium possessing high water retention comprises an upper layer and a lower layer, the lower layer is a resin gel layer containing high absorbent resin, the upper layer is a regular agar culture base covered on the resin gel layer; or is a resin gel layer containing the high absorbent resin. According to the invention, the high absorbent resin is used for modifying the microbe medium, by optimizing the medium preparation technology, the medium is obtained, compared with the common medium, the medium provided by the invention improves the disadvantages that the gel moisture is easy to volatilize, bacterium colony pollution is easy to form due to accumulation of condensate on the wall of a culture dish, crystallization or cracking is easy to be generated due to freezing, and the common medium is not beneficial to bacterial classification passage and long-term preservation. The cost of the product is reduced. The microbe medium solves the disadvantages of agar medium and medium dehydration in a microbe collection process. The medium has microbe growing property and biological compatibility, which is same with that of the common medium, and has water absorption performance, water retention performance, proper gel strength and transparency.

Description

technical field [0001] The invention relates to a microbial culture medium and a preparation method thereof, in particular to a microbial culture medium with high water retention and a preparation method thereof. Background technique [0002] Since the German bacteriologist Koch first used gelatin as a solid medium coagulant for bacterial streaking, the coagulant has played an important role in bacterial culture, isolation, and identification. Subsequently, each hydrophilic coagulant was discovered one after another, and after being dissolved in water, it swelled to form a viscous solution or gel, and was used in microbial culture medium. At present, most of the bacterial culture medium in the prior art is based on agar, and some nutrients required for bacterial growth are added, and then the bacteria are cultivated, observed and measured in a sterile laboratory according to strict aseptic operating procedures. Agar is the most widely used coagulant, but there are still pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14
Inventor 沈晓亮郭红樱陈欢叶俊英方序
Owner 浙江天科高新技术发展有限公司
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