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Preparation method of fungicide for anaerobic degradation of hydrochloric ether and application thereof

A technology of chlorinated hydrocarbons and degrading bacteria, applied in the field of biological treatment of groundwater pollution, can solve the problems of lack of microorganisms that degrade chlorinated hydrocarbons, restrict the development and application of chlorinated hydrocarbon-contaminated soil and groundwater anaerobic bioremediation technology, and achieve The effect of simple system design

Active Publication Date: 2013-12-04
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, chlorinated hydrocarbons are highly biotoxic, which often inhibits their biodegradability
At the same time, there may be a lack of microorganisms that degrade chlorinated hydrocarbons in the soil, which restricts the further development and application of anaerobic bioremediation technology for chlorinated hydrocarbon-contaminated soil and groundwater

Method used

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  • Preparation method of fungicide for anaerobic degradation of hydrochloric ether and application thereof
  • Preparation method of fungicide for anaerobic degradation of hydrochloric ether and application thereof
  • Preparation method of fungicide for anaerobic degradation of hydrochloric ether and application thereof

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Effect test

Embodiment 1

[0029] Embodiment 1, the preparation method of chlorinated hydrocarbon anaerobic degradation bacterial agent

[0030] To prepare the anaerobic degradation bacterial agent for hydrocarbons, the specific steps are as follows:

[0031] (1) Configuration of anaerobic medium, configure inorganic salt medium and add it to a 1 L Erlenmeyer flask, add 1 L of deionized water, wrap it with gauze and kraft paper, and put it in a high-pressure steam sterilizer at 121 oC high-pressure steam Sterilize for 20 minutes, take out the culture medium, and heat it until boiling, add resazurin indicator with a sterilized dropper until the solution turns blue;

[0032] (2) After the culture medium is cooled, replace the gauze and kraft paper with a sterilized rubber stopper matching the nitrogen filling device under aseptic conditions, open the high-purity nitrogen valve, and let the high-purity nitrogen pass through the closed heated copper Silk, further remove a small amount of oxygen inside, p...

Embodiment 2

[0041] TCA degradation characteristics under embodiment 2, producing methane condition

[0042] Utilize the flora domesticated by the method of the present invention to carry out TCA anaerobic degradation test, the methanogenic anaerobic degradation system is measured once every 7 days, 21 days is a cycle, draw the degradation curve under the methanogenic condition according to the measurement result as follows figure 1 Show. TCA degraded rapidly from 5 mg / L to 3.5 mg / L at 0-7 d, and the removal rate of TCA was 0.0429 mg / Ld, which was 30% removed; the degradation rate decreased at 7-14 d, and the concentration of TCA decreased from 3.5 mg / L decreased to 3.04 mg / L, the removal rate of TCA was 0.0 188 mg / Ld, and the removal rate was 9.2%; the degradation rate was the largest at 14-21 d, and the concentration of TCA decreased from 3.04 mg / L to 1.17 mg / L, and the removal rate of TCA The rate was 0.08788 mg / Ld, and 37.4% was removed; during the 21-day degradation process, the t...

Embodiment 3

[0043] TCA degradation characteristics under embodiment 3, nitrate reduction condition

[0044] Utilize the flora domesticated by the method of the present invention to carry out TCA anaerobic degradation test, the nitrate reduction anaerobic degradation system is measured once every 7 days, 21 days is a cycle, draw the degradation curve under the nitrate reduction condition according to the measurement result as follows figure 2 The results showed that TCA degraded rapidly from 5 mg / L to 3.32 mg / L at 0-7 d and 14-21 d, and the removal rate of TCA was 0.048 mg / Ld, which removed 33.6% and decreased from 2.626 mg / L decreased to 0.961 mg / L, the removal rate of TCA was 0.0405 mg / Ld, and the removal rate was 33.3%; the degradation rate slowed down after 7-14 days, and the concentration of TCA decreased from 3.32 mg / L to 2.626 mg / L, and the removal rate of TCA was 0.02986 mg / Ld, removal of 13.9%; in the 21-day degradation process, the total removal rate of TCA reached 80.8%, comp...

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Abstract

The invention discloses a preparation method of flora for anaerobic degradation of hydrochloric ether in the technical field of polluted soil and water biodegradation processing. The preparation method comprises the following steps of: adding hydrochloric ether polluted soil into a serum bottle filled with TCA and a basic medium for enrichment and domestication of hydrochloric ether anaerobic degraded microbe, adding a TCA standard liquid such that the concentration of TCA in the medium is 5mg / L, adding 5mL of H2, putting into a biochemical incubator at the temperature of 25 DEG C for anaerobic cultivation, and transferring for three times after a degradation curve tends to stabilize. After three times of transferring, solid particles in the culture bottle are basically removed, and anaerobic microbes are effectively enriched. The system provided by the invention is designed relatively simply, and has characteristics of high efficiency, economy and no secondary pollution for underground water polluted by organic matters. The invention provides a rapid and effective fungicide for degradation of hydrochloric ether and is an important link for in situ remediation.

Description

technical field [0001] The invention relates to the technology in the field of controlling groundwater pollution by using biological methods, and more specifically, relates to a preparation method and application of a chlorinated hydrocarbon anaerobic degradation bacterial agent. Background technique [0002] Chlorinated hydrocarbons refer to compounds in which hydrogen atoms in hydrocarbon molecules are replaced by chlorine atoms. They are rare in nature and most of them are artificially synthesized. Because chlorinated hydrocarbons are widely used in the production of factories, they have relatively serious pollution to the groundwater and soil environment. Chlorinated hydrocarbons are not only highly toxic, but also carcinogenic and mutagenic, and have strong toxic effects on the liver, kidney, cardiovascular and gastrointestinal organs of the human body, and also affect the central nervous system; and in the environment Difficult to degrade, these pose a great threat to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00B09C1/10C02F3/34C02F101/36
Inventor 李辉林匡飞刘勇弟吕树光周雅珍沈婷婷李璐陆强王晓丽
Owner EAST CHINA UNIV OF SCI & TECH
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