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Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity

A technology of biological fermentation and protein content, applied in animal feed, animal feed, application, etc., can solve problems such as restricting the application of cottonseed meal, and achieve the effect of reducing free gossypol content, lowering pH value, and improving taste

Active Publication Date: 2012-12-19
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the existence of these anti-nutritional factors, the application of cotton meal in livestock and poultry feed and aquatic feed is greatly restricted.

Method used

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  • Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity
  • Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity
  • Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Improve the crude protein content of cottonseed meal and the screening of strains that degrade gossypol

[0019] Raw cottonseed meal is derived from the by-products of cottonseed after oil extraction; the weight ratio of raw material to water is 1:0.8, and the prepared Aspergillus niger CICC41057 (purchased from China Institute of Food and Fermentation Industry), Candida utilis CICC 31188 (purchased from China Food Fermentation Industry Research Institute), Saccharomyces cerevisiae (CGMCC No.2388), Bacillus licheniformis (CGMCC No.5094), Lactobacillus salivarius CICC 21807 (purchased from China Food Fermentation Industry Research Institute) hospital); the fermentation temperature is 30°C, and the fermentation time is 48 hours. The test results are shown in Table 1.

[0020] Table 1 Effects of different bacterial strains on the crude protein content and gossypol of cottonseed meal

[0021]

[0022] According to the results, Aspergillus niger and Candida ut...

Embodiment 2

[0023] Example 2 Screening of bacterial strains that improve the mouthfeel of cotton meal

[0024] Raw cotton meal is derived from the by-products of cottonseed after oil extraction; the weight ratio of raw material to feed water is 1:0.8, and the prepared Bacillus subtilis (CGMCC No.4628), acidophilus milk bacillus (CGMCC No.5093), Enterococcus faecalis (CGMCC No.5092), Bacillus pumilus (CGMCC No.4756), Bacillus licheniformis (CGMCC No.5094), Lactobacillus salivarius CICC 21807; the fermentation temperature is 30°C, The fermentation time is 48 hours. The test results are shown in Table 2.

[0025] Table 2 Effects of different strains on the pH of cottonseed meal

[0026]

[0027] It can be seen from Table 2 that the pH of cotton meal fermented by Bacillus subtilis and Lactobacillus acidophilus is low, that is, more organic acids and other flavor substances are produced, so it is selected as the strain to improve the taste of cotton meal. Embodiment 3 Cotton Meal Biologi...

Embodiment 3

[0027] It can be seen from Table 2 that the pH of cotton meal fermented by Bacillus subtilis and Lactobacillus acidophilus is low, that is, more organic acids and other flavor substances are produced, so it is selected as the strain to improve the taste of cotton meal. Embodiment 3 Cotton Meal Biological Fermentation

[0028] Preparation of Aspergillus niger fermentation liquid: Inoculate Aspergillus niger into PDA slant medium, culture at 30°C for 72h, pick the mycelium on the slope, insert it into PDA liquid medium, and culture at 30°C for 16h with shaking at 150r / min.

[0029] Preparation of Candida utilis fermentation broth: insert Candida utilis into YPD slant medium, culture at 30°C for 48 hours, pick the lawn on the slant, insert it into YPD liquid medium, shake at 30°C, 150r / min Cultivate for 48h.

[0030] Preparation of Bacillus subtilis fermentation broth: Introduce Bacillus subtilis into LB slant medium, culture at 37°C for 24 hours, pick the bacterial lawn on the ...

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Abstract

The invention discloses a biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity. The method comprises the following steps: 1) respectively preparing fermentation broth of candida utilis, aspergillus niger, bacillus subtilis and lactobacillus acidophilus to serve as a strain; 2) preparing a raw material cottonseed meal; 3) adding water into the raw materials in a weight ratio of 1:0.5-1.2; 4) inoculating the strain prepared in the step 1), including 0.5 to 10 percent of the candida utilis, 0.5 to 1.2 percent of the aspergillus niger, 0.5 to 10 percent of the bacillus subtilis and 0.5 to 10 percent of the lactobacillus acidophilus; and 5) controlling the temperature to be between 25 and 40 DEG C for fermenting. The product obtained by the method directly can be used for effectively degrading free gossypol in the cottonseed meal, so that the protein quality of the cottonseed meal can be improved, the taste of the cottonseed meal can be improved, and the utilization of cottonseed meal in various livestock feed can be effectively increased.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation and relates to a biological fermentation method capable of increasing the protein content of cottonseed meal and capable of detoxification. Background technique [0002] Feed protein can be divided into animal protein and plant protein according to the source of raw materials. Among them, animal protein such as fish meal, meat and bone meal and blood meal has similar amino acid composition to the amino acid required for the growth of livestock and poultry, so this protein is not only The absorption rate is high, and it also has the advantages of fast absorption and less pollution. However, my country is a country that is extremely short of animal protein resources such as fishmeal. In particular, high-quality fishmeal mainly relies on imports, and the price is much higher than that of vegetable protein. In addition, due to the safety of animal protein, in recent years, people More ...

Claims

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Application Information

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IPC IPC(8): A23K1/14
Inventor 孙晓雯张军汪攀闫轶洁王安如
Owner BEIJING DABEINONG TECH GRP CO LTD
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