Method for improving softness and sensory quality of reconstituted tobacco by white rot fungus enzyme solution

A technology for reconstituting tobacco leaves and sensory quality, which is applied in the field of treating tobacco stem pulp with white rot fungus enzyme solution, can solve the problems of slow lignin degradation and low release amount, etc. Effect

Inactive Publication Date: 2012-12-19
HUBEI CHINA TOBACCO IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] There are currently three types of lignin oxidase systems known: 1. Lip-Mnp-Lac group, such as Coriolus Versicolor, Phlebiavadiate and Phanerochaete Chrysosporium, the latter is generally considered to contain no laccase, but some people think that it is because the medium is not suitable 2. Mnp-Lac groups, such as Lentinulaedodes and Panustigrinus, can modify lignin to increase water solubility, but CO 2 The release amount is not very high; 2. Lip-Lac group, such as Phlebiaochraceofulva, Junghuhunia Separabalima, the bacteria in this group degrade lignin very slowly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of white rot fungus T42 enzyme solution

[0024] The white rot fungus T42 enzyme solution is prepared as follows:

[0025] (1) Preparation of medium

[0026] The following substances are weighed and mixed according to the weight percentage.

[0027] ① PDA comprehensive agar medium: potato extract 2.0%, glucose 20.0%, KH 2 PO 4 3.0%, MgSO 4 ·7H 2 O2.0%, vitamin B 1 0.002%, agar 15.0%, ammonium tartrate 0.07%, use 1.0mol / L hydrochloric acid to adjust the pH to 5.0 for later use;

[0028] ② Enzyme production medium: anhydrous CaCl 2 0.08%, KH 2 PO 4 0.06%, anhydrous MgSO 4 0.10%, glucose 0.1%, ammonium tartrate 0.50%, ammonium nitrate 0.20%, vitamin B 1 0.002%, trace element mixture 0.001%, adjust the pH to 4.5 with acetic acid-sodium acetate buffer solution for later use;

[0029] ③ Trace element mixture: made of MnSO 4 , NaCl, ZnSO 4 , FeSO 4 ·7H 2 O and CuSO 4 ·5H 2 O is compounded with a concentration of 0.1%.

[0030] (2) Cultiva...

Embodiment 2

[0036] 1. Preparation of white rot fungus T42 enzyme solution:

[0037] The white rot fungus T42 enzyme solution is prepared as follows:

[0038] (1) Preparation of medium

[0039] The following substances are weighed and mixed according to the weight percentage.

[0040] ① PDA comprehensive agar medium: potato extract 3.0%, glucose 30.0%, KH 2 PO 4 2.0%, MgSO 4 ·7H 2 O3.0%, vitamin B 1 0.003%, agar 20.0%, ammonium tartrate 0.3%, regulate pH to 5.5 standby with the hydrochloric acid of 1.0mol / L;

[0041] ② Enzyme production medium: anhydrous CaCl 2 0.10%, KH 2 PO 4 0.05%, anhydrous MgSO 4 0.12%, glucose 0.2%, ammonium tartrate 0.30%, ammonium nitrate 0.40%, vitamin B 1 0.003%, trace element mixture 0.002%, adjust the pH to 5.5 with acetic acid-sodium acetate buffer solution for later use;

[0042] ③ Trace element mixture: made of ZnSO 4 , FeSO 4 ·7H 2 O, CuSO 4 ·5H 2 O. HBO 3 and NaMoO 4 2H 2 O is compounded with a concentration of 0.1%.

[0043] (2) Cu...

Embodiment 3

[0049] 1. Preparation of white rot fungus T42 enzyme solution:

[0050] The white rot fungus T42 enzyme solution is prepared as follows:

[0051] (1) Preparation of medium

[0052] The following substances are weighed and mixed according to the weight percentage.

[0053] ① PDA comprehensive agar medium: potato extract 1.0%, glucose 20.0%, KH 2 PO 4 3.0%, MgSO 4 ·7H 2 O 3.0%, vitamin B 1 0.003%, agar 10.0%, ammonium tartrate 1.0%, use 1.0mol / L hydrochloric acid to adjust the pH to 4.5 for later use;

[0054] ② Enzyme production medium: anhydrous CaCl 2 0.10%, KH 2 PO 4 0.04%, anhydrous MgSO 4 0.10%, glucose 0.1%, ammonium tartrate 0.20%, ammonium nitrate 0.70%, vitamin B 1 0.003%, trace element mixture 0.004%, adjust the pH to 6.0 with acetic acid-sodium acetate buffer solution for later use;

[0055] ③ Trace element mixture: made of MnSO 4 , NaCl, ZnSO 4 , FeSO 4 ·7H 2 O, CuSO 4 ·5H 2 O, A1K (SO 4 ) 2 12H 2 O. HBO 3 , CoC1 2 ·6H 2 O and NaMoO 4 2H...

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PUM

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Abstract

The invention discloses a method for improving softness and sensory quality of reconstituted tobacco by white rot fungus enzyme solution. Steps before and after a pulping phase of raw tobacco stem can be finished according to the existing technological process. The white rot fungus T42 enzyme solution accounting for 1.0-10.0% of dry weight of the raw tobacco stem is proportionally added during the pulping phase of the raw tobacco stem in production of the reconstituted tobacco. Lignin in the tobacco stem pulp is degraded at the stem pulp temperature of 30-80 DEG C under the pH value of 2.0-6.0 for 20-90 minutes. A strain of the white rot fungus T42 is named as coriolus versicolor T42 under the preservation number of CCTCC (china center for type culture collection) NO. M2012252. The method is reasonable, convenient to operate, evidently effective and capable of evidently improving softness of the reconstituted tobacco, sensory quality of the reconstituted tobacco is improved, wood gas is reduced, and irritation is reduced.

Description

technical field [0001] The invention belongs to the technical field of cigarette reconstituted tobacco leaves, and in particular relates to a method for treating tobacco stem slurry with white rot fungus enzyme solution in the production process of reconstituted tobacco leaves to improve the softness and sensory quality of reconstituted tobacco leaves. Background technique [0002] Reconstituted tobacco leaves are produced by using tobacco powder, tobacco stems, shredded tobacco pieces and other tobacco materials as raw materials, and are prepared through extraction, concentration, pulping, papermaking, and drying processes. Blending reconstituted tobacco leaves into cigarette products in a certain proportion can greatly reduce the consumption of single box production, improve the structure and combustion state of cigarettes, and reduce the release of tar and harmful components in cigarettes. [0003] At present, there is a certain gap between domestic reconstituted tobacco ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20
Inventor 毛耀刘志昌姚元军王凤兰王亮
Owner HUBEI CHINA TOBACCO IND
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