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Uniform polysaccharide of ganoderma sinensis liquid deep layer fermentation mycelium, and preparation method thereof

A technology of submerged liquid fermentation and mycelium, which is applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve problems that do not involve polysaccharide molecular weight, purity, and structural analysis, and achieve high polysaccharide content, The effect of high purity and low dosage

Inactive Publication Date: 2012-12-19
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of the above patent applications and articles involve research on the molecular weight, purity and structural analysis of polysaccharides

Method used

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  • Uniform polysaccharide of ganoderma sinensis liquid deep layer fermentation mycelium, and preparation method thereof
  • Uniform polysaccharide of ganoderma sinensis liquid deep layer fermentation mycelium, and preparation method thereof
  • Uniform polysaccharide of ganoderma sinensis liquid deep layer fermentation mycelium, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1: Preparation of the homogeneous polysaccharide GS-B-W-1 from Zizhi liquid submerged fermentation mycelium:

[0045] 1) Preparation of crude polysaccharide:

[0046] Ganoderma sinense liquid submerged fermentation mycelium (the Ganoderma sinense liquid submerged fermentation liquid is obtained by the method described in Chinese patent 200710045369.4 "A method of fermentation of Ganoderma sinense and the obtained Ganoderma mycelium"). The specific method is as follows: Ganoderma sinense ) was purchased from Shandong. Plate medium was sterilized at 121°C for 30 minutes, and cultured at 28°C for 4-6 days. Shake flask seed medium was sterilized at 121°C for 30 minutes, inoculated in a sterile room after cooling, and cultured with shaking on a shaker between shaker flasks at 28±1°C for 5-7 days, and the selected shaker flask seeds were put into the fermenter Cultivate for 5-6 days, and finally obtain the Zizhi liquid fermentation mycelia.

[0047] Extract the myc...

Embodiment 2

[0057] Example 2: Determination of the polysaccharide content of the homogeneous polysaccharide GS-B-W-1 of the Zizhi liquid submerged fermentation mycelia by the phenol-sulfuric acid method

[0058] Specific steps are as follows:

[0059] Preparation of standard product: Accurately weigh 25 mg of dried glucose, dissolve it to 100 ml, and prepare a 250 ug / ml standard product solution, take 0.05, 0.1, 0.15, 0.2, 0.25, and 0.3 mL in test tubes, add deionized water to make up 0.3mL, respectively add 5% phenol and 0.7mL and shake well, quickly add 4mL concentrated sulfuric acid, 50°C, 40min water bath, measure the absorbance after cooling.

[0060] Blank preparation: Take 0.6ml of deionized water, add 1.4ml of 5% phenol, 8ml of concentrated sulfuric acid, heat in a water bath, cool down, and make a blank solution.

[0061] Standard curve drawing: measure the absorbance value of standard test solution and blank solution at 488nm by UV spectrophotometry, and make a standard curve. ...

Embodiment 3

[0064] Example 3: Calibrate the molecular weight of the polysaccharide in the homogeneous polysaccharide GS-B-W-1 of the Zizhi liquid submerged fermentation mycelium of the present invention with the dextran standard product Dextron series

[0065] The specific method is as follows:

[0066] Chromatographic conditions: TSK-GEL GMPWxl chromatographic column; mobile phase: water; flow rate: 0.3mL / nin; column temperature: 35°C; detector: differential detector.

[0067] The standard Dextron and GS-B-W-1 samples were dissolved in water and injected.

[0068] Determine the retention time of dextran Dextron series and GS-B-W-1, use the retention time of Dextron-the molecular weight logarithmic value of Dextron series as a standard curve, and bring the retention time of GS-B-W-1 into the standard curve, it can be known that GS-B-W -1 is 7762.23 Daltons.

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Abstract

The invention discloses a uniform polysaccharide of ganoderma sinensis liquid deep layer fermentation mycelium. The uniform polysaccharide is characterized in that the total sugar content of the uniform polysaccharide is higher than 95%, and the molecular weight of the uniform polysaccharide is 3000-10000 daltons, preferably 7762.23 daltons. The invention also discloses a preparation method of the uniform polysaccharide. The uniform polysaccharide of the ganoderma sinensis liquid deep layer fermentation mycelium has higher content of polysaccharide, lower dosage of administration and higher purity; and the preparation process is suitable for industrialized production.

Description

technical field [0001] The present invention relates to the homogeneous polysaccharide GS-B-W-1 of Zizhi liquid submerged fermentation mycelium, its preparation method and its use in the preparation of antitumor drugs. Background technique [0002] Edible and medicinal fungi have various functions such as anti-tumor and anti-virus, lowering blood lipids, delaying aging, protecting the liver and detoxifying, promoting nucleic acid and protein biosynthesis, etc., and have a long history of use in China. A large number of studies at home and abroad have shown that fungal polysaccharides are isolated from fungal fruiting bodies, mycelia, and fermentation broths. They are the active parts of fungal drugs, and they are a type of active polysaccharides that can control cell division and differentiation, and regulate cell growth and aging. . [0003] Ganoderma sinense (Ganoderma sinense) is a fungus of the genus Ganoderma (2005 Chinese Pharmacopoeia), which has obvious anti-tumor a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P35/00
Inventor 杨义芳牛莉鑫金隽迪
Owner SHANGHAI INST OF PHARMA IND CO LTD
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