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Preparation method of animal peptone by double-enzyme enzymolysis

A technology of animal protein and double-enzyme enzymatic hydrolysis, applied in the field of biological products, can solve the problems of unstable technical performance indicators of clarity, acid-base stability, low amino acid content, affecting product application, etc., to ensure stability and high quality Sexuality, low cost, and the effect of increasing the depth of hydrolysis

Active Publication Date: 2012-12-19
PINGLIANG HUAKE BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Chinese patent 200510137997.6 discloses a preparation process of animal peptone, which uses a single compound protease to hydrolyze fresh bovine bone, bone marrow, animal tissue and viscera, and the obtained product has a large molecular weight and low amino acid content; especially the clarification of the product The technical performance indicators such as temperature and acid-base stability are unstable, which affects the application of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of pancreatin: mince fresh, impurity-free and oil-free pure porcine pancreas or bovine pancreas with a meat grinder, add 6-8% of pancreas weight and 98% by mass edible ethanol, and use Stir well with a beater and place it for 1~6h; add water 1.5~2 times the weight of the pancreas and stir evenly, put it in a constant temperature room at 35~45°C, measure the enzyme activity every 5h until the enzyme activity reaches 10~13 One million orders, ready to use.

[0024] (2) Preparation of animal peptone

[0025] Materials: 85kg of fresh beef bone, 15kg of animal tissue and viscera;

[0026] Preparation process: Wash the fresh beef bone with high-pressure water to make it bloodless, then put it into an autoclave, add 200kg of deionized, control the steam pressure at 1.5Mpa, cook for 10 hours, separate the bone glue liquid, cool it, and remove the oil; the animal After cleaning the tissues and viscera, grind them with an industrial meat grinder, add them to the...

Embodiment 2

[0033] (1) Preparation of trypsin: same as Example 1.

[0034] (2) Preparation of peptone:

[0035] Materials: 90kg of fresh beef bone, 10kg of animal tissue and viscera;

[0036] Preparation process: Wash the fresh beef bone with high-pressure water to make it bloodless, then put it into an autoclave, add 300kg of deionized, control the steam pressure at 2.0Mpa, cook for 8 hours, separate the bone glue, cool it, and remove the oil; the animal After cleaning the tissues and viscera, grind them with an industrial meat grinder, add them to the above-mentioned bone glue solution, adjust the pH to 8.0 with caustic soda solution, raise the temperature to about 50°C, and add 2.5% of the total weight of fresh beef bones, animal tissues and viscera trypsin, stirred for 2.5 hours; then added compound protease with 1.2% of the total amount of raw materials, stirred for 1.5 hours; 70°C, add activated carbon or activated kaolin for decolorization; the decolorized enzymatic solution is f...

Embodiment 3

[0039] (1) Preparation of trypsin: same as Example 1.

[0040] (2) Preparation of peptone:

[0041] Materials: 88kg of fresh beef bone, 12kg of animal tissue and viscera;

[0042]Preparation process: Wash the fresh beef bone with high-pressure water to make it bloodless, then put it into an autoclave, add 250kg of deionized, control the steam pressure at 2.5Mpa, cook for 6 hours, separate the bone glue liquid, cool it, and remove the oil; the animal After cleaning the tissue and viscera, grind them with an industrial meat grinder, add them to the above-mentioned bone glue solution, adjust the pH to 9.0 with caustic soda solution, raise the temperature to about 45°C, add 3.5% of the total weight of fresh beef bones, animal tissues and viscera trypsin, the hydrolysis time is 2 hours, then add compound protease of 1% of the total amount of raw materials, and stir the enzymolysis for 2 hours; ℃, add activated carbon or activated kaolin for decolorization; the decolorized enzymat...

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Abstract

The invention provides a preparation method of animal peptone by double-enzyme enzymolysis and belongs to the technical field of biological products. Fresh ox bone and animal tissue and entrails are taken as the raw materials and are subjected to deep hydrolysis by adopting pancreatin and compound protease under a weak base condition, and then the technical measures of separation and purification and the like are carried out to obtain a peptone of which the molecular weight is steadily kept between 2000 and 2500 and of which the amino acid content is balanced. The characteristics of high efficiency, specifity, distribution cooperativity, environment friendliness and the like obtained through catalytic hydrolysis by pancreatin and compound protease are fully utilized, so that stability and the high quality of various performance indexes of products are effectively ensured. The obtained peptone does not precipitate and is not muddy in the presence of acid and base and the peptone is not solidified and keeps completely clear at a high temperature. The peptone can be used as the main raw material of high-grade microorganism mediums and can completely replace imported products.

Description

technical field [0001] The invention relates to the technical field of biological products, and relates to a method for preparing animal peptone, in particular to a method for preparing animal peptone by enzymatic hydrolysis with two enzymes. Background technique [0002] Peptone is a pale yellow powder obtained by hydrolyzing meat, casein or gelatin with acid or protease and drying. It has a special smell of meat. Peptone can also be formed after protein is decomposed by acid, alkali or protease. One of the primary digestion products of protein in the stomach is peptone. Peptones are rich in organic nitrogen compounds and also contain some vitamins and sugars. It can be used as the main raw material of microbial culture medium, and it is widely used in the fields of clinical testing, antibiotics, pharmaceutical industry, fermentation industry, biochemical products and microbiology research, and can be used to treat digestive tract diseases; different organisms need Speci...

Claims

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Application Information

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IPC IPC(8): C12P21/06
Inventor 员长安
Owner PINGLIANG HUAKE BIOLOGICAL TECH
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