Method for measuring toxicity of sterilizing agent on brassica rapa pekinensis peronospora parasitica
A technology of Chinese cabbage and fungicides, which is applied in the field of determining the toxicity of fungicides to Chinese cabbage downy mildew and the determination of the toxicity of fungicides to pathogenic bacteria, and can solve the problems of inconsistent concentration of pathogenic bacteria inoculum, lack of comparability and reference value, and repeated experiments problems such as poor performance and poor accuracy, and achieve the effect of great social and economic significance, good parallelism, and less test materials
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Embodiment 1
[0040] Embodiment 1, the selection of each treatment condition in the toxicity of fungicide to Chinese cabbage downy mildew by cotyledon soaking method
[0041] 1. Selection of growth stages of Chinese cabbage
[0042] Take the leaves of Chinese cabbage plants at different development stages (cotyledon stage, seedling stage and rosette stage), and perform the following operations respectively:
[0043] Leaves at the cotyledon stage: Rinse the cotyledons with sterile water for 3 times, then place them neatly on a ф150mm petri dish moistened with absorbent paper, with the back facing up, with 10 cotyledons per dish, and inoculate 10 μL of sporangia of downy mildew of Chinese cabbage on the back of each cotyledon Suspension (1 x 10 4 sporangia / mL), placed in a 15°C, 12h light-dark alternating light incubator for moisturizing culture.
[0044] Leaves at the seedling stage: Rinse the leaves 3 times with sterile water, then place them neatly with the back side up in a transparent ...
Embodiment 2
[0085] The precision test of the method that embodiment 2, embodiment establish
[0086] The six Chinese cabbage downy mildew strains used in this example are voluntarily isolated, and the strain names are JB8, YS7, ZB1, QD4, JB13 and BJ2 respectively.
[0087] The isolation method of Chinese cabbage downy mildew strains is as follows: collect the downy mildew leaves that occurred on the Chinese cabbage seed strains in the field, rinse the mold layer and attached organisms on the surface with tap water, and place them at 18°C and 100% relative humidity. Under dark conditions, cultivate in enamel trays for 24 hours, and store them in a -20°C refrigerator after a fresh mold layer grows. For specific methods, please refer to the literature: Wang Cuihua, Wang Hongjiu, Wang Shixiang. Preliminary research on the preservation method of Chinese cabbage downy mildew .Shandong Agricultural Science, 1989,4:38.
[0088] 1. Prepare 95% azoxystrobin original drug with sterile water to ma...
Embodiment 3
[0098] Embodiment 3, cross-resistance test between bactericides
[0099] The six Chinese cabbage downy mildew strains used in this example are self-isolated, and the strain names are JB8, JB5, JN, JB2, GD1 and JB6 respectively. The isolation method of Chinese cabbage downy mildew bacterial strain is the same as embodiment 2.
[0100] The toxicity of three fungicides, cymoxanil, propamocarb and azoxystrobin, to six Chinese cabbage downy mildew strains were tested respectively, and the specific steps were as follows:
[0101] 1. Prepare the bactericide with sterile water to make a series of concentration gradient liquid medicine.
[0102] Among them, 99% propamocarb original drug was prepared into respective drug solutions with active ingredient concentrations of 0.05, 0.1, 0.5, 1, 2, 5, 5 μg / mL.
[0103] Among them, the 99% cymoxanil original drug was prepared into respective drug solutions with active ingredient concentrations of 0.05, 0.1, 0.5, 1, 2, 5, 5 μg / mL.
[0104] A...
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