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Method for microalgae coupling of culturing and recovery to quickly accumulate algal oil

A technology of microalgae culture and microalgae culture solution, which is applied in the field of microalgae bioenergy, can solve the problems of high energy consumption for harvesting, waste of culture salt and water, increase of culture cost, etc., to simplify the operation process, shorten production time, The effect of saving cultivation costs

Active Publication Date: 2012-12-26
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Centrifugal harvesting requires large equipment investment and high harvesting energy consumption
Flocculation harvesting often adopts the method of adding a flocculant to the algae liquid to promote the flocculation and sedimentation of the algal cells. Due to the addition of the flocculant, the supernatant after harvesting the microalgae cannot be recycled for the cultivation of the microalgae, resulting in the loss of culture salt and water. Waste, which increases the cost of cultivation. At the same time, the supernatant after flocculation is rich in flocculants, nutrients and algae cells. Direct discharge will pollute the environment, and purification treatment will increase the cost.

Method used

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  • Method for microalgae coupling of culturing and recovery to quickly accumulate algal oil
  • Method for microalgae coupling of culturing and recovery to quickly accumulate algal oil
  • Method for microalgae coupling of culturing and recovery to quickly accumulate algal oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The concentration of nitrogen and the optimization of nitrogen-phosphorus ratio in the improved F / 2 culture solution of embodiment 1

[0042] Adding NaNO to basal F / 2 medium 3 And adjust the mass ratio of nitrogen and phosphorus to obtain the improved F / 2 culture solution, divide the experiment into 11 groups:

[0043] Group 1 is the control group, and the culture medium is the basic F / 2 culture medium, that is, NaNO 3 The final concentration is 75mg / L, NaH 2 PO 4 2H 2 The final concentration of O is 5 mg / L, and the corresponding nitrogen to phosphorus mass ratio is 12:1;

[0044] Group 2~Group 11 is the experimental group, and the culture medium of Group 2~Group 7 is the improved F / 2 culture medium, NaNO 3 The final concentrations are 25, 75, 225, 450, 900, 1350 mg / L, corresponding to NaH 2 PO 4 2H 2 The final concentrations of O were 5, 15, 45, 90, 180, and 270 mg / L, respectively, to ensure that the mass ratio of nitrogen and phosphorus was 4:1. The culture m...

Embodiment 2

[0051] Example 2 Condition optimization of the coupling process of Nannochloropsis pH stress oil production and pH flocculation recovery

[0052] Adjustment of NaNO in basal F / 2 medium 3 The final concentration is 225mg / L, and the mass ratio of nitrogen and phosphorus is 4:1 (corresponding to NaH 2 PO 4 2H 2 O final concentration was 45mg / L), and improved F / 2 culture solution was obtained.

[0053] (1) Inoculate fresh cells of Nannochloropsis ophthalmica into the above-mentioned modified F / 2 culture medium at an inoculum volume fraction of 5% at a culture temperature of 25-30°C, under natural light, and continuously inflate with air for 3 days to After the logarithmic phase, it was cultured for another 15 days under the same conditions to obtain the algae liquid. Take 1000mL of algae liquid, adjust the pH of the algae liquid to 11, let it stand at room temperature (25~30°C) for 3 days to separate the algae liquid, obtain the supernatant and the lower layer of precipitation...

Embodiment 3

[0067] Example 3 pH flocculation The supernatant after harvesting microalgae is recycled for the cultivation of microalgae

[0068] Adjustment of NaNO in basal F / 2 medium 3 The final concentration is 225mg / L, and the mass ratio of nitrogen and phosphorus is 4:1 (corresponding to NaH 2 PO 4 2H 2 O final concentration was 45mg / L), and improved F / 2 culture solution was obtained.

[0069] (1) Inoculate fresh cells of Nannochloropsis ophthalmica into the above-mentioned modified F / 2 culture medium at an inoculum volume fraction of 5% at a culture temperature of 25-30°C, under natural light, and continuously inflate with air for 3 days to After the logarithmic phase, it was cultured for another 15 days under the same conditions to obtain the algae liquid. Adjust the pH of the algae solution to 11, and let it stand at room temperature (25~30°C) for 3 days to separate the algae solution to obtain the supernatant and the lower layer of sediment. ), and recycle the supernatant.

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Abstract

The invention discloses a method for coupling of microalgae culturing and recovery to quickly accumulate algal oil. The method includes inoculating fresh microalgae cells to a corresponding microalgae culture solution to obtain algal liquid; adjusting the potential of hydrogen (pH) value of the algal liquid, and standing for layering; and supernatant liquor is recovered and circulatedto be used for the microalgae culturing, and underlayer sedimentation is used for extracting the algal oil by means of leaching with an organic solvent or Soxhlet extraction or an acid method. According to the method, in the earlier stage of growth of the microalgae, by means of adjustment of concentrations of nitrogen and phosphorus in the nutrient solution, biomass of the microalgae is improved; in the later stage of the growth of the microalgae, by means of adjustment of the pH value, an environment of strong base or strong acid is formed, the microalgae is stressed to quickly accumulate the algal oil, meanwhile the microalgae is in flocculation dewatering, and the microalgae is recovered; and therefore, the culturing and the recovery are coupled, the operation process is simplified, and production time is shortened, and cyclic utilization of the nutrient solution not only saves culturing cost, but also avoids pollution of discharging of the culture solution to the environment.

Description

(1) Technical field [0001] The invention belongs to the technical field of microalgae bioenergy, and relates to a coupling method for rapidly accumulating a large amount of algae oil through stress culture of oil-producing microalgae and harvesting of the microalgae. (2) Background technology [0002] With the rapid development of industry and economy, the problem of energy shortage and environmental pollution is becoming more and more serious. Seeking renewable and clean energy that can replace petroleum has become the development direction of the world's energy industry. Algae have the characteristics of high photosynthetic efficiency, short growth cycle, strong environmental adaptability, and rich algae oil content, and are one of the promising raw materials for bioenergy production. At present, the key problems of microalgae bioenergy are the high cost of cultivation and the difficulty in harvesting microalgae. There are two main reasons for the high cost of cultivation...

Claims

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Application Information

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IPC IPC(8): C12N1/12C11B1/10C11B1/04C11B1/00C12R1/89
Inventor 陆向红计建炳窦晓卢美贞
Owner ZHEJIANG UNIV OF TECH
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