Method for realizing protective reaction for hydroxyl groups of cytidine compound through catalysis of pseudomonas fluorescens

Abstract

The invention discloses a method for realizing a protective reaction for hydroxyl groups of a cytidine compound through catalysis of pseudomonas fluorescens. According to the method, the usage amount of a cell catalyst is 40 to 80 mg / mL; the mol ratio of the cytidine compound to an esterification reagent is 1: 20 to 1: 50; the cell catalyst, the cytidine compound and the esterification reagent are added into an organic solvent for a reaction for 24 to 72 h under the conditions of a temperature of 20 to 50 DEG C, initial water content of 1 to 5%, an oscillation speed of 50 to 300 rpm and a normal pressure so as to obtain the cytidine compound with esterified and protected 5'-hydroxyl groups. The method provided by the invention realizes the protective reaction for the 5'-hydroxyl groups in one step and has the advantages of low cost, mild reaction conditions, environment-friendliness and simple and controllable reaction process.

Description

technical field [0001] The invention belongs to the field of biocatalysis and organic synthesis, and in particular relates to a method for catalyzing the 5'-hydroxyl protection reaction of cytidine compounds by Pseudomonas fluorescens. Background technique [0002] The protection of specific groups of multifunctional compounds is a common step in organic synthesis. Through this reaction method, some active groups that do not want to participate in the reaction, such as carbonyl, hydroxyl, amino, etc., can be protected, so that such groups can temporarily Being blunted does not affect subsequent reactions. In addition, the group protection reaction in organic synthesis is also one of the important strategies to improve the reaction selectivity. The regioselectivity of the reaction can be improved by passivating some groups, and even the stereoselectivity can be achieved. [0003] Cytidine compounds are typical polyhydroxy compounds, and are also an important class of compoun...

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the disadvantages of using toxic chemical catalysts or green but high-cost enzyme catalysts in the industrial application of the current group protection reaction of cytidine compounds, and aims to provide a cytidine compound catalyzed by Pseudomonas fluorescens cells The method of hydroxyl protection reaction

The raw materials of this method are cheap and easy to purchase, and the linked enzyme on the Pseudomonas fluorescens cells is used to catalyze the reaction without separation and purification of the enzyme. The regioselectivity of the reaction to the secondary hydroxyl group of cytidine compounds reaches more than 80%, which overcomes the traditional The low selectivity of the chemical method leads to low substrate utilization, low product purity, and easy generation of by-products.