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Detecting kit for hepatitis a virus IgM and IgG antibodies through colloidal gold method

A technology of hepatitis A virus and detection kit, which is applied in the direction of measuring devices, resistance to vector-borne diseases, instruments, etc., to achieve the effect of easy operation, fast response, and pain relief

Inactive Publication Date: 2013-01-02
上海博沃生物科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

In the detection method of colloidal gold, there is the report of detecting hepatitis A virus IgM or IgG separately, but the detection kit that detects hepatitis A virus IgM and IgG antibody simultaneously with colloidal gold class immunochromatographic technique has not been reported

Method used

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Embodiment

[0022] lMain material

[0023] 1.1 Hepatitis A virus-specific VP1 antigen and VP1 antibody: products of Shenzhen Faipeng Biological Co., Ltd., which are recombinant antigens, VP1 antigen is used for colloidal gold labeling, and VP1 antibody is used for NC membrane quality control line coating;

[0024] Mouse anti-human IgM and IgG antibodies: Arista products, used for NC membrane coating;

[0025] Chlorauric acid: product of Sigma company;

[0026] Nitrocellulose (NC) membrane: product of Millipore;

[0027] Bovine serum albumin (BSA), polyethylene glycol PEG20000, hydrolyzed casein: Sigma product.

[0028] Other commonly used reagents are analytical reagents.

[0029] 1.2 The clinical serum was obtained by the company in relevant hospitals, including 50 samples positive for IgG antibody to hepatitis A virus and 15 samples positive for IgM antibody, including 8 samples positive for both antibodies. Both antibodies were negative in 60 normal samples.

[0030] 1.3 Hepatitis...

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PUM

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Abstract

The invention discloses a detecting kit for hepatitis a virus IgM and IgG antibodies through a colloidal gold method. The detecting kit comprises a kit body, sample diluting liquid and detecting test paper. The detecting test paper is composed of a plastic supporting plate, a sample feeding pad, a colloidal gold pad of antigen VP1 marked with hepatitis a virus specifity, a nitrocellulose NC film, a detecting line T1, a detecting line T2, a quality control C line and a sample absorbing pad and arranged in the kit body. The kit detects that IgM and IgG in serum of the sample are positive simultaneously, the sample result is completely identical with an enzyme-linked immunosorbent assay (ELISA) reagent, no remarkable difference exists, and the kit is applicable to clinical detecting. The kit is low in cost and simple in preparation process and has practical value.

Description

technical field [0001] The invention relates to the detection of hepatitis A virus, in particular to a colloidal gold method detection kit for hepatitis A virus IgM and IgG antibodies and a preparation method thereof. [0002] Background technique [0003] The pathogen of hepatitis A (hepatitis A, referred to as HA) is hepatitis A virus (HAV), which is a small RNA virus (picornavirus) with a diameter of 27 nm. The virus exists in the patient's feces, serum, bile, and liver cytoplasm. It has strong resistance in vitro and can survive being heated at 60°C for 4 hours; it can tolerate the environment of pH 3.0; it can survive for a long time at low temperature. Experiments have shown that HAV has relative resistance to the effects of ether, heating at 60°C for 1 hour and pH3 (it can survive for several months at 4°C). However, it can be inactivated by heating at 100°C for 5 minutes and treating it with formaldehyde solution, chlorine, etc. Non-ionic detergents do not destroy...

Claims

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Application Information

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IPC IPC(8): G01N33/576G01N33/531G01N33/543
CPCY02A50/30
Inventor 吴克史晋刘昊智鲍路伟王文灏程超张爱晖
Owner 上海博沃生物科技有限公司
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